FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116414064> ?p ?o ?g. }

• W2116414064 endingPage "2643" @default.
• W2116414064 startingPage "2637" @default.
• W2116414064 abstract "Gluconate:NADP 5-oxidoreductase (GNO) from the acetic acid bacterium Gluconobacter oxydans subsp. oxydans DSM3503 was purified to homogeneity. This enzyme is involved in the nonphosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. GNO was localized in the cytoplasm, had an isoelectric point of 4.3, and showed an apparent molecular weight of 75,000. In sodium dodecyl sulfate gel electrophoresis, a single band appeared corresponding to a molecular weight of 33,000, which indicated that the enzyme was composed of two identical subunits. The pH optimum of gluconate oxidation was pH 10, and apparent Km values were 20.6 mM for the substrate gluconate and 73 microM for the cosubstrate NADP. The enzyme was almost inactive with NAD as a cofactor and was very specific for the substrates gluconate and 5-ketogluconate. D-Glucose, D-sorbitol, and D-mannitol were not oxidized, and 2-ketogluconate and L-sorbose were not reduced. Only D-fructose was accepted, with a rate that was 10% of the rate of 5-ketogluconate reduction. The gno gene encoding GNO was identified by hybridization with a gene probe complementary to the DNA sequence encoding the first 20 N-terminal amino acids of the enzyme. The gno gene was cloned on a 3.4-kb DNA fragment and expressed in Escherichia coli. Sequencing of the gene revealed an open reading frame of 771 bp, encoding a protein of 257 amino acids with a predicted relative molecular mass of 27.3 kDa. Plasmid-encoded gno was functionally expressed, with 6.04 U/mg of cell-free protein in E. coli and with 6.80 U/mg of cell-free protein in G. oxydans, which corresponded to 85-fold overexpression of the G. oxydans wild-type GNO activity. Multiple sequence alignments showed that GNO was affiliated with the group II alcohol dehydrogenases, or short-chain dehydrogenases, which display a typical pattern of six strictly conserved amino acid residues." @default.
• W2116414064 created "2016-06-24" @default.
• W2116414064 creator A5009712853 @default.
• W2116414064 creator A5010848908 @default.
• W2116414064 creator A5079348954 @default.
• W2116414064 date "1995-05-01" @default.
• W2116414064 modified "2023-10-10" @default.
• W2116414064 title "Biochemical characterization and sequence analysis of the gluconate:NADP 5-oxidoreductase gene from Gluconobacter oxydans" @default.
• W2116414064 cites W1040079625 @default.
• W2116414064 cites W1127888983 @default.
• W2116414064 cites W1478492937 @default.
• W2116414064 cites W1484252392 @default.
• W2116414064 cites W1529218396 @default.
• W2116414064 cites W1535514846 @default.
• W2116414064 cites W1750773183 @default.
• W2116414064 cites W1934359967 @default.
• W2116414064 cites W1964488033 @default.
• W2116414064 cites W1972264162 @default.
• W2116414064 cites W1975085160 @default.
• W2116414064 cites W1982012231 @default.
• W2116414064 cites W1987326847 @default.
• W2116414064 cites W1996285227 @default.
• W2116414064 cites W2018289835 @default.
• W2116414064 cites W2019410656 @default.
• W2116414064 cites W2025801832 @default.
• W2116414064 cites W2028622989 @default.
• W2116414064 cites W2032118018 @default.
• W2116414064 cites W2036231150 @default.
• W2116414064 cites W2047043554 @default.
• W2116414064 cites W2050599642 @default.
• W2116414064 cites W2050864355 @default.
• W2116414064 cites W2054979333 @default.
• W2116414064 cites W2057203378 @default.
• W2116414064 cites W2060721922 @default.
• W2116414064 cites W2063450941 @default.
• W2116414064 cites W2073553628 @default.
• W2116414064 cites W2085474307 @default.
• W2116414064 cites W2089377780 @default.
• W2116414064 cites W2095181192 @default.
• W2116414064 cites W2100837269 @default.
• W2116414064 cites W2103776170 @default.
• W2116414064 cites W2114624600 @default.
• W2116414064 cites W2123949437 @default.
• W2116414064 cites W2126432677 @default.
• W2116414064 cites W2128635872 @default.
• W2116414064 cites W2138270253 @default.
• W2116414064 cites W2143071715 @default.
• W2116414064 cites W2161524167 @default.
• W2116414064 cites W2168858682 @default.
• W2116414064 cites W2226671716 @default.
• W2116414064 cites W2364245667 @default.
• W2116414064 cites W2490701537 @default.
• W2116414064 cites W4248096216 @default.
• W2116414064 cites W4254901942 @default.
• W2116414064 cites W4293247451 @default.
• W2116414064 cites W87371739 @default.
• W2116414064 cites W2091764115 @default.
• W2116414064 doi "https://doi.org/10.1128/jb.177.10.2637-2643.1995" @default.
• W2116414064 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/176932" @default.
• W2116414064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7751271" @default.
• W2116414064 hasPublicationYear "1995" @default.
• W2116414064 type Work @default.
• W2116414064 sameAs 2116414064 @default.
• W2116414064 citedByCount "53" @default.
• W2116414064 countsByYear W21164140642012 @default.
• W2116414064 countsByYear W21164140642013 @default.
• W2116414064 countsByYear W21164140642014 @default.
• W2116414064 countsByYear W21164140642015 @default.
• W2116414064 countsByYear W21164140642016 @default.
• W2116414064 countsByYear W21164140642017 @default.
• W2116414064 countsByYear W21164140642019 @default.
• W2116414064 countsByYear W21164140642020 @default.
• W2116414064 countsByYear W21164140642022 @default.
• W2116414064 crossrefType "journal-article" @default.
• W2116414064 hasAuthorship W2116414064A5009712853 @default.
• W2116414064 hasAuthorship W2116414064A5010848908 @default.
• W2116414064 hasAuthorship W2116414064A5079348954 @default.
• W2116414064 hasBestOaLocation W21164140641 @default.
• W2116414064 hasConcept C104317684 @default.
• W2116414064 hasConcept C153911025 @default.
• W2116414064 hasConcept C167625842 @default.
• W2116414064 hasConcept C181199279 @default.
• W2116414064 hasConcept C197957613 @default.
• W2116414064 hasConcept C2776673659 @default.
• W2116414064 hasConcept C2777858148 @default.
• W2116414064 hasConcept C2779268744 @default.
• W2116414064 hasConcept C2780388412 @default.
• W2116414064 hasConcept C515207424 @default.
• W2116414064 hasConcept C547475151 @default.
• W2116414064 hasConcept C55493867 @default.
• W2116414064 hasConcept C58121356 @default.
• W2116414064 hasConcept C75520062 @default.
• W2116414064 hasConcept C86803240 @default.
• W2116414064 hasConceptScore W2116414064C104317684 @default.
• W2116414064 hasConceptScore W2116414064C153911025 @default.
• W2116414064 hasConceptScore W2116414064C167625842 @default.
• W2116414064 hasConceptScore W2116414064C181199279 @default.
• W2116414064 hasConceptScore W2116414064C197957613 @default.

• next