Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116425580> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2116425580 endingPage "e22" @default.
- W2116425580 startingPage "e22" @default.
- W2116425580 abstract "<h3>Background</h3> During ischaemia, AMPK activation occurs in order to provide energy from alternative resources. However, AMPK activity is known to be impaired in diabetes. We hypothesised that enhancing AMPK activation above physiological levels during ischaemia would protect both the normoglycaemic and the diabetic heart. <h3>Methodology</h3> Hearts from Wistar and Goto Kakizaki rats (GK, a mildly diabetic rat strain) were subjected to 35 min coronary artery occlusion in the presence of 10, 20 or 40 μM A-769662 (an activator of AMPK), followed by 120 min of reperfusion with normal buffer (n ≥ 6). Risk zone and myocardial infarction were assessed using Evans blue and 2,3,5-triphenyltetrazolium chloride (TTC) staining, respectively and expressed as percentage of the area at risk (I/R%). The effect of A-769662 on mitochondrial permeability transition (opening of the mPT pore is associated with reperfusion injury) was also investigated by exposing rat cardiomyocytes loaded with the fluorophore TMRM to a laser oxidative insult; the time to mitochondrial membrane depolarisation and rigour contracture were measured (n=6, 80–100 cells/assay). <h3>Results</h3> A-769662 reduced the infarct size in both the normoglycaemic and diabetic hearts in comparison with control hearts at 20 μM (31.8%±3.1 vs 51.4%±1.5 normoglycaemic heart; 22.7%±3.0 vs 37.6%±2.7 for the GK heart; p<0.05) and at 40 μM (35.6%±1.9 vs 51.4%±1.5 for the normoglycaemic heart; 18.6%±1.6 vs 37.6%±2.7, for the GK heart; p<0.05) In addition, A-769662 also significantly delayed the mPTP opening (147.71%±10.2% at 20 μM, 146.7%±15.6% at 40 μM, vs control 100%, p<0.05). <h3>Conclusions</h3> Our data suggest that the enhancement of AMPK activity during ischaemia may lead to infarct reduction and delayed opening of the mPTP in the ischaemic reperfused rat heart." @default.
- W2116425580 created "2016-06-24" @default.
- W2116425580 creator A5014011356 @default.
- W2116425580 creator A5038080240 @default.
- W2116425580 creator A5071151797 @default.
- W2116425580 creator A5071555509 @default.
- W2116425580 creator A5083191627 @default.
- W2116425580 creator A5087656523 @default.
- W2116425580 date "2010-08-26" @default.
- W2116425580 modified "2023-09-30" @default.
- W2116425580 title "BAS/BSCR34 Is an increased AMPK activation during ischaemia essential for the protection of the heart against infarction?" @default.
- W2116425580 doi "https://doi.org/10.1136/hrt.2010.205781.45" @default.
- W2116425580 hasPublicationYear "2010" @default.
- W2116425580 type Work @default.
- W2116425580 sameAs 2116425580 @default.
- W2116425580 citedByCount "0" @default.
- W2116425580 crossrefType "journal-article" @default.
- W2116425580 hasAuthorship W2116425580A5014011356 @default.
- W2116425580 hasAuthorship W2116425580A5038080240 @default.
- W2116425580 hasAuthorship W2116425580A5071151797 @default.
- W2116425580 hasAuthorship W2116425580A5071555509 @default.
- W2116425580 hasAuthorship W2116425580A5083191627 @default.
- W2116425580 hasAuthorship W2116425580A5087656523 @default.
- W2116425580 hasBestOaLocation W21164255801 @default.
- W2116425580 hasConcept C126322002 @default.
- W2116425580 hasConcept C134018914 @default.
- W2116425580 hasConcept C164705383 @default.
- W2116425580 hasConcept C181199279 @default.
- W2116425580 hasConcept C185592680 @default.
- W2116425580 hasConcept C2777798775 @default.
- W2116425580 hasConcept C2779768347 @default.
- W2116425580 hasConcept C2780124434 @default.
- W2116425580 hasConcept C500558357 @default.
- W2116425580 hasConcept C541997718 @default.
- W2116425580 hasConcept C55493867 @default.
- W2116425580 hasConcept C555293320 @default.
- W2116425580 hasConcept C71924100 @default.
- W2116425580 hasConcept C97029542 @default.
- W2116425580 hasConceptScore W2116425580C126322002 @default.
- W2116425580 hasConceptScore W2116425580C134018914 @default.
- W2116425580 hasConceptScore W2116425580C164705383 @default.
- W2116425580 hasConceptScore W2116425580C181199279 @default.
- W2116425580 hasConceptScore W2116425580C185592680 @default.
- W2116425580 hasConceptScore W2116425580C2777798775 @default.
- W2116425580 hasConceptScore W2116425580C2779768347 @default.
- W2116425580 hasConceptScore W2116425580C2780124434 @default.
- W2116425580 hasConceptScore W2116425580C500558357 @default.
- W2116425580 hasConceptScore W2116425580C541997718 @default.
- W2116425580 hasConceptScore W2116425580C55493867 @default.
- W2116425580 hasConceptScore W2116425580C555293320 @default.
- W2116425580 hasConceptScore W2116425580C71924100 @default.
- W2116425580 hasConceptScore W2116425580C97029542 @default.
- W2116425580 hasIssue "17" @default.
- W2116425580 hasLocation W21164255801 @default.
- W2116425580 hasOpenAccess W2116425580 @default.
- W2116425580 hasPrimaryLocation W21164255801 @default.
- W2116425580 hasRelatedWork W1968813451 @default.
- W2116425580 hasRelatedWork W1993316077 @default.
- W2116425580 hasRelatedWork W2068108356 @default.
- W2116425580 hasRelatedWork W2080285792 @default.
- W2116425580 hasRelatedWork W2108241812 @default.
- W2116425580 hasRelatedWork W2137971781 @default.
- W2116425580 hasRelatedWork W2361173162 @default.
- W2116425580 hasRelatedWork W2407212191 @default.
- W2116425580 hasRelatedWork W4239141576 @default.
- W2116425580 hasRelatedWork W3031236773 @default.
- W2116425580 hasVolume "96" @default.
- W2116425580 isParatext "false" @default.
- W2116425580 isRetracted "false" @default.
- W2116425580 magId "2116425580" @default.
- W2116425580 workType "article" @default.