FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116437973> ?p ?o ?g. }

• W2116437973 endingPage "5019" @default.
• W2116437973 startingPage "5012" @default.
• W2116437973 abstract "KCNQ2 and KCNQ3 potassium-channel subunits can form both homomeric and heteromeric channels; the latter are thought to constitute native ganglionic M channels. We have tried to deduce the stoichiometric contributions of KCNQ2 and KCNQ3 subunits to currents generated by the coexpression of KCNQ2 and KCNQ3 cDNA plasmids in Chinese hamster ovary (CHO) cells, and to native M currents in dissociated rat superior cervical ganglion (SCG) neurons, by comparing the block of these currents produced by tetraethylammonium (TEA) with the block of currents generated by a tandem KCNQ3/2 construct. TEA concentration-inhibition curves against coexpressed KCNQ2 plus KCNQ3 currents, and against native M currents in SCG neurons from 6-week-old [postnatal day 45 (P45)] rats, were indistinguishable from those for the expressed tandem construct, and fully accorded with a 1:1 stoichiometry. Inhibition curves in neurons from younger (P17) rats could be better fitted assuming an additional small proportion of current carried by KCNQ2 homomultimers. Single-cell PCR yielded signals for KCNQ2, KCNQ3, and KCNQ5 mRNAs in all SCG neurons tested from both P17 and P45 rats. Quantitative PCR of whole-ganglion mRNA revealed stable levels of KCNQ2 and KCNQ5 mRNA between P7 and P45, but excess and incrementing levels of KCNQ3 mRNA. Increasing levels of KCNQ3 protein between P17 and P45 were confirmed by immunocytochemistry. We conclude that coexpressed KCNQ2 plus KCNQ3 cDNAs generate channels with 1:1 (KCNQ2:KCNQ3) stoichiometry in CHO cells and that native M channels in SCG neurons adopt the same conformation during development, assisted by the increased expression of KCNQ3 mRNA and protein." @default.
• W2116437973 created "2016-06-24" @default.
• W2116437973 creator A5002141514 @default.
• W2116437973 creator A5010524977 @default.
• W2116437973 creator A5015103768 @default.
• W2116437973 creator A5024385167 @default.
• W2116437973 creator A5027714194 @default.
• W2116437973 creator A5031407472 @default.
• W2116437973 creator A5034952565 @default.
• W2116437973 date "2003-06-15" @default.
• W2116437973 modified "2023-10-11" @default.
• W2116437973 title "Stoichiometry of Expressed KCNQ2/KCNQ3 Potassium Channels and Subunit Composition of Native Ganglionic M Channels Deduced from Block by Tetraethylammonium" @default.
• W2116437973 cites W1535902137 @default.
• W2116437973 cites W1554749896 @default.
• W2116437973 cites W1575439657 @default.
• W2116437973 cites W1664352025 @default.
• W2116437973 cites W1892342982 @default.
• W2116437973 cites W1964272828 @default.
• W2116437973 cites W1973027891 @default.
• W2116437973 cites W1984966634 @default.
• W2116437973 cites W1997537641 @default.
• W2116437973 cites W2003303798 @default.
• W2116437973 cites W2011565078 @default.
• W2116437973 cites W2013413397 @default.
• W2116437973 cites W2019942525 @default.
• W2116437973 cites W2025884619 @default.
• W2116437973 cites W2042515922 @default.
• W2116437973 cites W2045457678 @default.
• W2116437973 cites W2046404285 @default.
• W2116437973 cites W2053967009 @default.
• W2116437973 cites W2058346833 @default.
• W2116437973 cites W2059206044 @default.
• W2116437973 cites W2059690890 @default.
• W2116437973 cites W2066008360 @default.
• W2116437973 cites W2071453469 @default.
• W2116437973 cites W2073653273 @default.
• W2116437973 cites W2073985563 @default.
• W2116437973 cites W2079291010 @default.
• W2116437973 cites W2080615794 @default.
• W2116437973 cites W2101625991 @default.
• W2116437973 cites W2107449228 @default.
• W2116437973 cites W2123921221 @default.
• W2116437973 cites W2137046511 @default.
• W2116437973 cites W2140894987 @default.
• W2116437973 cites W2145684092 @default.
• W2116437973 cites W2163824920 @default.
• W2116437973 cites W2164578725 @default.
• W2116437973 doi "https://doi.org/10.1523/jneurosci.23-12-05012.2003" @default.
• W2116437973 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6741196" @default.
• W2116437973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12832524" @default.
• W2116437973 hasPublicationYear "2003" @default.
• W2116437973 type Work @default.
• W2116437973 sameAs 2116437973 @default.
• W2116437973 citedByCount "110" @default.
• W2116437973 countsByYear W21164379732012 @default.
• W2116437973 countsByYear W21164379732013 @default.
• W2116437973 countsByYear W21164379732014 @default.
• W2116437973 countsByYear W21164379732015 @default.
• W2116437973 countsByYear W21164379732016 @default.
• W2116437973 countsByYear W21164379732017 @default.
• W2116437973 countsByYear W21164379732018 @default.
• W2116437973 countsByYear W21164379732019 @default.
• W2116437973 countsByYear W21164379732020 @default.
• W2116437973 countsByYear W21164379732021 @default.
• W2116437973 countsByYear W21164379732022 @default.
• W2116437973 crossrefType "journal-article" @default.
• W2116437973 hasAuthorship W2116437973A5002141514 @default.
• W2116437973 hasAuthorship W2116437973A5010524977 @default.
• W2116437973 hasAuthorship W2116437973A5015103768 @default.
• W2116437973 hasAuthorship W2116437973A5024385167 @default.
• W2116437973 hasAuthorship W2116437973A5027714194 @default.
• W2116437973 hasAuthorship W2116437973A5031407472 @default.
• W2116437973 hasAuthorship W2116437973A5034952565 @default.
• W2116437973 hasBestOaLocation W21164379731 @default.
• W2116437973 hasConcept C104292427 @default.
• W2116437973 hasConcept C104317684 @default.
• W2116437973 hasConcept C12554922 @default.
• W2116437973 hasConcept C153911025 @default.
• W2116437973 hasConcept C170493617 @default.
• W2116437973 hasConcept C175656101 @default.
• W2116437973 hasConcept C178790620 @default.
• W2116437973 hasConcept C185592680 @default.
• W2116437973 hasConcept C2776104367 @default.
• W2116437973 hasConcept C2780918503 @default.
• W2116437973 hasConcept C2781295685 @default.
• W2116437973 hasConcept C517785266 @default.
• W2116437973 hasConcept C55493867 @default.
• W2116437973 hasConcept C83743174 @default.
• W2116437973 hasConcept C86803240 @default.
• W2116437973 hasConceptScore W2116437973C104292427 @default.
• W2116437973 hasConceptScore W2116437973C104317684 @default.
• W2116437973 hasConceptScore W2116437973C12554922 @default.
• W2116437973 hasConceptScore W2116437973C153911025 @default.
• W2116437973 hasConceptScore W2116437973C170493617 @default.
• W2116437973 hasConceptScore W2116437973C175656101 @default.
• W2116437973 hasConceptScore W2116437973C178790620 @default.
• W2116437973 hasConceptScore W2116437973C185592680 @default.
• W2116437973 hasConceptScore W2116437973C2776104367 @default.

• next