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- W2116502180 abstract "ABSTRACT Haemophilus influenzae efficiently colonizes and persists at the human nasopharyngeal mucosa, causing disease when it spreads to other sites. Nitric oxide (NO) represents a major antimicrobial defense deployed by host cells in locations colonized by H. influenzae during pathogenesis that are likely to vary in oxygen levels. Formate-dependent nitrite reductase regulator (FNR) is an oxygen-sensitive regulator in several bacterial pathogens. We report that fnr of H. influenzae is required for anaerobic defense against exposure to NO donors and to resist NO-dependent effects of gamma interferon (IFN-γ)-activated murine bone marrow-derived macrophages. To understand the mechanism of resistance, we investigated the role of FNR-regulated genes in defense against NO sources. Expression analysis revealed FNR-dependent activation of nrfA , dmsA , napA , and ytfE . Nonpolar deletion mutants of nrfA and ytfE exhibited sensitivity to NO donors, and the ytfE gene was more critical for survival. Compared to the wild-type strain, the ytfE mutant exhibited decreased survival when exposed to macrophages, a defect that was more pronounced after prior stimulation of macrophages with IFN-γ or lipopolysaccharide. Complementation restored survival of the mutant to the level in the parental strain. Increased sensitivity of the ytfE mutant relative to that of the parent was abrogated by treatment of macrophages with a NO synthase inhibitor, implicating YtfE in resistance to a NO-dependent pathway. These results identify a requirement for FNR in positive control of ytfE and indicate a critical role for ytfE in resistance of H. influenzae to reactive nitrogen species and the antibacterial effects of macrophages." @default.
- W2116502180 created "2016-06-24" @default.
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- W2116502180 creator A5090287874 @default.
- W2116502180 date "2009-05-01" @default.
- W2116502180 modified "2023-10-03" @default.
- W2116502180 title "Resistance of<i>Haemophilus influenzae</i>to Reactive Nitrogen Donors and Gamma Interferon-Stimulated Macrophages Requires the Formate-Dependent Nitrite Reductase Regulator-Activated<i>ytfE</i>Gene" @default.
- W2116502180 cites W1443898972 @default.
- W2116502180 cites W1486766426 @default.
- W2116502180 cites W1496717884 @default.
- W2116502180 cites W1507090507 @default.
- W2116502180 cites W1584128039 @default.
- W2116502180 cites W1652028084 @default.
- W2116502180 cites W1834398632 @default.
- W2116502180 cites W1896982977 @default.
- W2116502180 cites W1910873220 @default.
- W2116502180 cites W1963012831 @default.
- W2116502180 cites W1969630923 @default.
- W2116502180 cites W1971403296 @default.
- W2116502180 cites W1971873200 @default.
- W2116502180 cites W1987005877 @default.
- W2116502180 cites W1989488774 @default.
- W2116502180 cites W1989695975 @default.
- W2116502180 cites W1990139307 @default.
- W2116502180 cites W1990527302 @default.
- W2116502180 cites W1992825941 @default.
- W2116502180 cites W1997081772 @default.
- W2116502180 cites W1998686777 @default.
- W2116502180 cites W2000619939 @default.
- W2116502180 cites W2011237115 @default.
- W2116502180 cites W2011582460 @default.
- W2116502180 cites W2011885345 @default.
- W2116502180 cites W2014198590 @default.
- W2116502180 cites W2014455970 @default.
- W2116502180 cites W2016648215 @default.
- W2116502180 cites W2022467415 @default.
- W2116502180 cites W2026551617 @default.
- W2116502180 cites W2027229943 @default.
- W2116502180 cites W2030086291 @default.
- W2116502180 cites W2030959690 @default.
- W2116502180 cites W2033162758 @default.
- W2116502180 cites W2035643252 @default.
- W2116502180 cites W2038704829 @default.
- W2116502180 cites W2045074472 @default.
- W2116502180 cites W2045684223 @default.
- W2116502180 cites W2057402362 @default.
- W2116502180 cites W2058480912 @default.
- W2116502180 cites W2065216540 @default.
- W2116502180 cites W2085551318 @default.
- W2116502180 cites W2090383135 @default.
- W2116502180 cites W2091936669 @default.
- W2116502180 cites W2097922773 @default.
- W2116502180 cites W2099364155 @default.
- W2116502180 cites W2099627512 @default.
- W2116502180 cites W2102658219 @default.
- W2116502180 cites W2111984347 @default.
- W2116502180 cites W2116175149 @default.
- W2116502180 cites W2118225952 @default.
- W2116502180 cites W2120620942 @default.
- W2116502180 cites W2123647584 @default.
- W2116502180 cites W2123983151 @default.
- W2116502180 cites W2124345503 @default.
- W2116502180 cites W2126135631 @default.
- W2116502180 cites W2126524143 @default.
- W2116502180 cites W2127289590 @default.
- W2116502180 cites W2127739733 @default.
- W2116502180 cites W2129871259 @default.
- W2116502180 cites W2135353569 @default.
- W2116502180 cites W2135546669 @default.
- W2116502180 cites W2136065859 @default.
- W2116502180 cites W2137576203 @default.
- W2116502180 cites W2138197344 @default.
- W2116502180 cites W2138457121 @default.
- W2116502180 cites W2138792025 @default.
- W2116502180 cites W2146171755 @default.
- W2116502180 cites W2146771855 @default.
- W2116502180 cites W2147776446 @default.
- W2116502180 cites W2149200346 @default.
- W2116502180 cites W2152718829 @default.
- W2116502180 cites W2155331964 @default.
- W2116502180 cites W2155477248 @default.
- W2116502180 cites W2158718811 @default.
- W2116502180 cites W2161248611 @default.
- W2116502180 cites W2161917059 @default.
- W2116502180 cites W2162427919 @default.
- W2116502180 cites W2162483715 @default.
- W2116502180 cites W2163293935 @default.
- W2116502180 cites W2167294225 @default.
- W2116502180 cites W2167636782 @default.
- W2116502180 cites W2169346653 @default.
- W2116502180 cites W2171315493 @default.
- W2116502180 cites W4232417638 @default.
- W2116502180 doi "https://doi.org/10.1128/iai.01365-08" @default.
- W2116502180 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2681730" @default.