FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116546550> ?p ?o ?g. }

• W2116546550 endingPage "8612" @default.
• W2116546550 startingPage "8601" @default.
• W2116546550 abstract "Although in vitro replication of the hepatitis C virus (HCV) JFH1 clone of genotype 2a (HCVcc) has been developed, a robust cell culture system for the 1a and 1b genotypes, which are the most prevalent viruses in the world and resistant to interferon therapy, has not yet been established. As a surrogate virus system, pseudotype viruses transiently bearing HCV envelope proteins based on the vesicular stomatitis virus (VSV) and retrovirus have been developed. Here, we have developed a replication-competent recombinant VSV with a genome encoding unmodified HCV E1 and E2 proteins in place of the VSV envelope protein (HCVrv) in human cell lines. HCVrv and a pseudotype VSV bearing the unmodified HCV envelope proteins (HCVpv) generated in 293T or Huh7 cells exhibited high infectivity in Huh7 cells. Generation of infectious HCVrv was limited in some cell lines examined. Furthermore, HCVrv but not HCVpv was able to propagate and form foci in Huh7 cells. The infection of Huh7 cells with HCVpv and HCVrv was neutralized by anti-hCD81 and anti-E2 antibodies and by sera from chronic HCV patients. The infectivity of HCVrv was inhibited by an endoplasmic reticulum alpha-glucosidase inhibitor, N-(n-nonyl) deoxynojirimycin (Nn-DNJ), but not by a Golgi mannosidase inhibitor, deoxymannojirimycin. Focus formation of HCVrv in Huh7 cells was impaired by Nn-DNJ treatment. These results indicate that the HCVrv developed in this study can be used to study HCV envelope proteins with respect to not only the biological functions in the entry process but also their maturation step." @default.
• W2116546550 created "2016-06-24" @default.
• W2116546550 creator A5014211191 @default.
• W2116546550 creator A5014582936 @default.
• W2116546550 creator A5022553058 @default.
• W2116546550 creator A5038192407 @default.
• W2116546550 creator A5044702232 @default.
• W2116546550 creator A5053053781 @default.
• W2116546550 creator A5056616389 @default.
• W2116546550 creator A5057079653 @default.
• W2116546550 creator A5058411918 @default.
• W2116546550 creator A5075892037 @default.
• W2116546550 creator A5087349637 @default.
• W2116546550 creator A5087837717 @default.
• W2116546550 creator A5091292240 @default.
• W2116546550 creator A5019206138 @default.
• W2116546550 date "2007-08-15" @default.
• W2116546550 modified "2023-09-24" @default.
• W2116546550 title "Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins" @default.
• W2116546550 cites W1712519623 @default.
• W2116546550 cites W1933267078 @default.
• W2116546550 cites W1963905773 @default.
• W2116546550 cites W1964786592 @default.
• W2116546550 cites W1965513749 @default.
• W2116546550 cites W1968837330 @default.
• W2116546550 cites W1979255492 @default.
• W2116546550 cites W1980511942 @default.
• W2116546550 cites W1981745840 @default.
• W2116546550 cites W1986505810 @default.
• W2116546550 cites W1987287280 @default.
• W2116546550 cites W1987982858 @default.
• W2116546550 cites W1993690048 @default.
• W2116546550 cites W2003579943 @default.
• W2116546550 cites W2005411001 @default.
• W2116546550 cites W2008860633 @default.
• W2116546550 cites W2012420380 @default.
• W2116546550 cites W2015489330 @default.
• W2116546550 cites W2017359325 @default.
• W2116546550 cites W2022077416 @default.
• W2116546550 cites W2030371794 @default.
• W2116546550 cites W2035774172 @default.
• W2116546550 cites W2042994480 @default.
• W2116546550 cites W2044193462 @default.
• W2116546550 cites W2048162805 @default.
• W2116546550 cites W2052588752 @default.
• W2116546550 cites W2053032921 @default.
• W2116546550 cites W2055822853 @default.
• W2116546550 cites W2062741582 @default.
• W2116546550 cites W2063495032 @default.
• W2116546550 cites W2068764615 @default.
• W2116546550 cites W2071136569 @default.
• W2116546550 cites W2075147482 @default.
• W2116546550 cites W2090297731 @default.
• W2116546550 cites W2097084266 @default.
• W2116546550 cites W2105891409 @default.
• W2116546550 cites W2110842901 @default.
• W2116546550 cites W2116192835 @default.
• W2116546550 cites W2116885823 @default.
• W2116546550 cites W2117084310 @default.
• W2116546550 cites W2118320694 @default.
• W2116546550 cites W2119303435 @default.
• W2116546550 cites W2121169051 @default.
• W2116546550 cites W2121476095 @default.
• W2116546550 cites W2122043154 @default.
• W2116546550 cites W2124339485 @default.
• W2116546550 cites W2128839291 @default.
• W2116546550 cites W2129810176 @default.
• W2116546550 cites W2130904640 @default.
• W2116546550 cites W2132341678 @default.
• W2116546550 cites W2134181802 @default.
• W2116546550 cites W2141322335 @default.
• W2116546550 cites W2141626800 @default.
• W2116546550 cites W2144361201 @default.
• W2116546550 cites W2147126871 @default.
• W2116546550 cites W2152238363 @default.
• W2116546550 cites W2153686676 @default.
• W2116546550 cites W2153744339 @default.
• W2116546550 cites W2159159412 @default.
• W2116546550 cites W2159770243 @default.
• W2116546550 cites W2160174559 @default.
• W2116546550 cites W2167044622 @default.
• W2116546550 cites W2168597289 @default.
• W2116546550 cites W2169601765 @default.
• W2116546550 cites W4252500291 @default.
• W2116546550 doi "https://doi.org/10.1128/jvi.00608-07" @default.
• W2116546550 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1951354" @default.
• W2116546550 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17553880" @default.
• W2116546550 hasPublicationYear "2007" @default.
• W2116546550 type Work @default.
• W2116546550 sameAs 2116546550 @default.
• W2116546550 citedByCount "75" @default.
• W2116546550 countsByYear W21165465502012 @default.
• W2116546550 countsByYear W21165465502013 @default.
• W2116546550 countsByYear W21165465502014 @default.
• W2116546550 countsByYear W21165465502015 @default.
• W2116546550 countsByYear W21165465502016 @default.
• W2116546550 countsByYear W21165465502017 @default.
• W2116546550 countsByYear W21165465502018 @default.

• next