Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116565903> ?p ?o ?g. }
- W2116565903 endingPage "241" @default.
- W2116565903 startingPage "232" @default.
- W2116565903 abstract "To date, the biological role of prokaryotic K(+) channels remains unknown. Helicobacter pylori contains a gene encoding a putative K(+) channel (HpKchA) of the two-transmembrane RCK (regulation of K(+) conductance) domain family, but lacks known bacterial K(+) uptake systems. A H. pylori DeltahpKchA mutant presented a strong growth defect at low K(+) concentration, which was compensated by KCl addition. The role of the separate RCK domain was investigated in H. pylori by mutagenesis of its internal start codon, which led to a K(+)-dependent intermediate growth phenotype, consistent with RCK activating channel function. Tagging HpKchA C-terminally, we detected a 1:1 stoichiometry of the full-length HpKchA and the separate RCK domain. We constructed single amino-acid exchanges within the unusual selectivity filter of HpKchA (ATGFGA) in H. pylori and observed complete loss (G74A), a slight defect (G76A or F75G) or wild-type (A77D) channel function. HpKchA was essential for colonization of the murine stomach. These data show, for the first time, a biological function for a prokaryotic K(+) channel, as a K(+) uptake system, essential for the persistence of H. pylori in the gastric environment." @default.
- W2116565903 created "2016-06-24" @default.
- W2116565903 creator A5002940746 @default.
- W2116565903 creator A5017518614 @default.
- W2116565903 creator A5025003953 @default.
- W2116565903 creator A5038501444 @default.
- W2116565903 creator A5039680714 @default.
- W2116565903 creator A5045153197 @default.
- W2116565903 creator A5048243430 @default.
- W2116565903 creator A5052817365 @default.
- W2116565903 creator A5087191671 @default.
- W2116565903 creator A5090824461 @default.
- W2116565903 date "2006-12-07" @default.
- W2116565903 modified "2023-10-18" @default.
- W2116565903 title "Channel-mediated potassium uptake in Helicobacter pylori is essential for gastric colonization" @default.
- W2116565903 cites W110534461 @default.
- W2116565903 cites W1575542444 @default.
- W2116565903 cites W166113645 @default.
- W2116565903 cites W1896452697 @default.
- W2116565903 cites W1978775427 @default.
- W2116565903 cites W1983184954 @default.
- W2116565903 cites W1984640298 @default.
- W2116565903 cites W2006670481 @default.
- W2116565903 cites W2008481607 @default.
- W2116565903 cites W2012031105 @default.
- W2116565903 cites W2029851838 @default.
- W2116565903 cites W2031226921 @default.
- W2116565903 cites W2032812821 @default.
- W2116565903 cites W2039501975 @default.
- W2116565903 cites W2047265762 @default.
- W2116565903 cites W2067322161 @default.
- W2116565903 cites W2076999300 @default.
- W2116565903 cites W2077873945 @default.
- W2116565903 cites W2077922814 @default.
- W2116565903 cites W2082362903 @default.
- W2116565903 cites W2083123834 @default.
- W2116565903 cites W2083231222 @default.
- W2116565903 cites W2083397106 @default.
- W2116565903 cites W2094349878 @default.
- W2116565903 cites W2096514941 @default.
- W2116565903 cites W2099604214 @default.
- W2116565903 cites W2101204196 @default.
- W2116565903 cites W2101871915 @default.
- W2116565903 cites W2109258309 @default.
- W2116565903 cites W2109803384 @default.
- W2116565903 cites W2110132014 @default.
- W2116565903 cites W2111695324 @default.
- W2116565903 cites W2118216537 @default.
- W2116565903 cites W2120904752 @default.
- W2116565903 cites W2124635565 @default.
- W2116565903 cites W2143070723 @default.
- W2116565903 cites W2143140859 @default.
- W2116565903 cites W2144624514 @default.
- W2116565903 cites W2148175992 @default.
- W2116565903 cites W2151718252 @default.
- W2116565903 cites W2153269862 @default.
- W2116565903 cites W2306093346 @default.
- W2116565903 cites W2317076510 @default.
- W2116565903 cites W2479598873 @default.
- W2116565903 cites W4239833500 @default.
- W2116565903 cites W4242040237 @default.
- W2116565903 cites W59088806 @default.
- W2116565903 doi "https://doi.org/10.1038/sj.emboj.7601471" @default.
- W2116565903 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1782367" @default.
- W2116565903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17159901" @default.
- W2116565903 hasPublicationYear "2006" @default.
- W2116565903 type Work @default.
- W2116565903 sameAs 2116565903 @default.
- W2116565903 citedByCount "35" @default.
- W2116565903 countsByYear W21165659032013 @default.
- W2116565903 countsByYear W21165659032014 @default.
- W2116565903 countsByYear W21165659032015 @default.
- W2116565903 countsByYear W21165659032016 @default.
- W2116565903 countsByYear W21165659032017 @default.
- W2116565903 countsByYear W21165659032018 @default.
- W2116565903 countsByYear W21165659032019 @default.
- W2116565903 countsByYear W21165659032020 @default.
- W2116565903 countsByYear W21165659032021 @default.
- W2116565903 countsByYear W21165659032022 @default.
- W2116565903 countsByYear W21165659032023 @default.
- W2116565903 crossrefType "journal-article" @default.
- W2116565903 hasAuthorship W2116565903A5002940746 @default.
- W2116565903 hasAuthorship W2116565903A5017518614 @default.
- W2116565903 hasAuthorship W2116565903A5025003953 @default.
- W2116565903 hasAuthorship W2116565903A5038501444 @default.
- W2116565903 hasAuthorship W2116565903A5039680714 @default.
- W2116565903 hasAuthorship W2116565903A5045153197 @default.
- W2116565903 hasAuthorship W2116565903A5048243430 @default.
- W2116565903 hasAuthorship W2116565903A5052817365 @default.
- W2116565903 hasAuthorship W2116565903A5087191671 @default.
- W2116565903 hasAuthorship W2116565903A5090824461 @default.
- W2116565903 hasBestOaLocation W21165659031 @default.
- W2116565903 hasConcept C104317684 @default.
- W2116565903 hasConcept C118892022 @default.
- W2116565903 hasConcept C12554922 @default.
- W2116565903 hasConcept C127716648 @default.
- W2116565903 hasConcept C14036430 @default.
- W2116565903 hasConcept C143065580 @default.