FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116618362> ?p ?o ?g. }

• W2116618362 endingPage "4416" @default.
• W2116618362 startingPage "4410" @default.
• W2116618362 abstract "Insulin receptor substrate 1 (IRS-1), an intracellular substrate of the insulin receptor tyrosine kinase, also is heavily phosphorylated on serine and threonine residues, and several serine phosphorylation sites alter the function of IRS-1. Because of the large number of serine/threonine residues, position-by-position analysis of these potential phosphorylation sites by mutagenesis is difficult. To circumvent this, we have employed matrix-assisted laser desorption/ionization time-of-flight and HPLC-electrospray ionization tandem mass spectrometry techniques to scan for serine and threonine residues that are phosphorylated in full-length human IRS-1 ectopically expressed in cells using an adenoviral vector. This approach revealed 12 phosphorylation sites on serine or threonine residues, 10 of which were novel sites. Seven of these sites were in proline-directed motifs, whereas five were in arginine-directed sites. Sequence inspection suggested that phosphorylation of Ser1223 might alter the interaction of IRS-1 with the protein tyrosine phosphatase Src homology domain 2 (SH2)-containing phosphatase-2 (SHP-2). Mutation of Ser1223 to alanine to prevent phosphorylation resulted in increased association of SHP-2 with IRS-1, decreased insulin-stimulated tyrosine phosphorylation of IRS-1 in CHO/IR cells, and decreased insulin-stimulated association of the p85 regulatory subunit of phosphatidylinositol-3-kinase with IRS-1. This mutation had no effect on association of IRS-1 with the insulin receptor. Sequence analysis showed the Ser1223 region to be widely conserved evolutionarily. These data suggest that phosphorylation of Ser1223 dampens association of IRS-1 with SHP-2, thereby increasing net insulin-stimulated tyrosine phosphorylation." @default.
• W2116618362 created "2016-06-24" @default.
• W2116618362 creator A5005466573 @default.
• W2116618362 creator A5010955181 @default.
• W2116618362 creator A5014904840 @default.
• W2116618362 creator A5037831264 @default.
• W2116618362 creator A5039848911 @default.
• W2116618362 creator A5046813621 @default.
• W2116618362 creator A5060153927 @default.
• W2116618362 creator A5060951188 @default.
• W2116618362 creator A5089720468 @default.
• W2116618362 date "2005-10-01" @default.
• W2116618362 modified "2023-09-30" @default.
• W2116618362 title "Identification of Insulin Receptor Substrate 1 Serine/Threonine Phosphorylation Sites Using Mass Spectrometry Analysis: Regulatory Role of Serine 1223" @default.
• W2116618362 cites W1554860529 @default.
• W2116618362 cites W1570362997 @default.
• W2116618362 cites W1602425018 @default.
• W2116618362 cites W1972590376 @default.
• W2116618362 cites W1975299036 @default.
• W2116618362 cites W1981072221 @default.
• W2116618362 cites W1999971397 @default.
• W2116618362 cites W2003659422 @default.
• W2116618362 cites W2011210301 @default.
• W2116618362 cites W2017584173 @default.
• W2116618362 cites W2017602160 @default.
• W2116618362 cites W2022008301 @default.
• W2116618362 cites W2039498210 @default.
• W2116618362 cites W2045926149 @default.
• W2116618362 cites W2049113370 @default.
• W2116618362 cites W2049225751 @default.
• W2116618362 cites W2049875806 @default.
• W2116618362 cites W2050247932 @default.
• W2116618362 cites W2053691240 @default.
• W2116618362 cites W2064801081 @default.
• W2116618362 cites W2066569500 @default.
• W2116618362 cites W2097688893 @default.
• W2116618362 cites W2097838137 @default.
• W2116618362 cites W2102683312 @default.
• W2116618362 cites W2115177689 @default.
• W2116618362 cites W2120852510 @default.
• W2116618362 cites W2124948826 @default.
• W2116618362 cites W2130850001 @default.
• W2116618362 cites W2135766812 @default.
• W2116618362 cites W2139191784 @default.
• W2116618362 cites W2147825853 @default.
• W2116618362 cites W2152211462 @default.
• W2116618362 cites W2153266804 @default.
• W2116618362 cites W2161071101 @default.
• W2116618362 cites W2170678561 @default.
• W2116618362 doi "https://doi.org/10.1210/en.2005-0260" @default.
• W2116618362 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16020478" @default.
• W2116618362 hasPublicationYear "2005" @default.
• W2116618362 type Work @default.
• W2116618362 sameAs 2116618362 @default.
• W2116618362 citedByCount "57" @default.
• W2116618362 countsByYear W21166183622012 @default.
• W2116618362 countsByYear W21166183622013 @default.
• W2116618362 countsByYear W21166183622014 @default.
• W2116618362 countsByYear W21166183622016 @default.
• W2116618362 countsByYear W21166183622017 @default.
• W2116618362 countsByYear W21166183622018 @default.
• W2116618362 countsByYear W21166183622019 @default.
• W2116618362 countsByYear W21166183622020 @default.
• W2116618362 countsByYear W21166183622022 @default.
• W2116618362 countsByYear W21166183622023 @default.
• W2116618362 crossrefType "journal-article" @default.
• W2116618362 hasAuthorship W2116618362A5005466573 @default.
• W2116618362 hasAuthorship W2116618362A5010955181 @default.
• W2116618362 hasAuthorship W2116618362A5014904840 @default.
• W2116618362 hasAuthorship W2116618362A5037831264 @default.
• W2116618362 hasAuthorship W2116618362A5039848911 @default.
• W2116618362 hasAuthorship W2116618362A5046813621 @default.
• W2116618362 hasAuthorship W2116618362A5060153927 @default.
• W2116618362 hasAuthorship W2116618362A5060951188 @default.
• W2116618362 hasAuthorship W2116618362A5089720468 @default.
• W2116618362 hasBestOaLocation W21166183621 @default.
• W2116618362 hasConcept C112446052 @default.
• W2116618362 hasConcept C11960822 @default.
• W2116618362 hasConcept C134018914 @default.
• W2116618362 hasConcept C153911025 @default.
• W2116618362 hasConcept C185592680 @default.
• W2116618362 hasConcept C185967709 @default.
• W2116618362 hasConcept C201624660 @default.
• W2116618362 hasConcept C2775880066 @default.
• W2116618362 hasConcept C2776414213 @default.
• W2116618362 hasConcept C2777391703 @default.
• W2116618362 hasConcept C2777553839 @default.
• W2116618362 hasConcept C2779306644 @default.
• W2116618362 hasConcept C55493867 @default.
• W2116618362 hasConcept C85528070 @default.
• W2116618362 hasConcept C86803240 @default.
• W2116618362 hasConcept C87325107 @default.
• W2116618362 hasConcept C97029542 @default.
• W2116618362 hasConceptScore W2116618362C112446052 @default.
• W2116618362 hasConceptScore W2116618362C11960822 @default.
• W2116618362 hasConceptScore W2116618362C134018914 @default.
• W2116618362 hasConceptScore W2116618362C153911025 @default.
• W2116618362 hasConceptScore W2116618362C185592680 @default.

• next