Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116675226> ?p ?o ?g. }
- W2116675226 endingPage "3963" @default.
- W2116675226 startingPage "3955" @default.
- W2116675226 abstract "An alpha-galactosidase gene from Thermus brockianus ITI360 was cloned, sequenced, and expressed in Escherichia coli, and the recombinant protein was purified. The gene, designated agaT, codes for a 476-residue polypeptide with a calculated molecular mass of 53, 810 Da. The native structure of the recombinant enzyme (AgaT) was estimated to be a tetramer. AgaT displays amino acid sequence similarity to the alpha-galactosidases of Thermotoga neapolitana and Thermotoga maritima and a low-level sequence similarity to alpha-galactosidases of family 36 in the classification of glycosyl hydrolases. The enzyme is thermostable, with a temperature optimum of activity at 93 degrees C with para-nitrophenyl-alpha-galactopyranoside as a substrate. Half-lives of inactivation at 92 and 80 degrees C are 100 min and 17 h, respectively. The pH optimum is between 5.5 and 6.5. The enzyme displayed high affinity for oligomeric substrates. The K(m)s for melibiose and raffinose at 80 degrees C were determined as 4.1 and 11.0 mM, respectively. The alpha-galactosidase gene in T. brockianus ITI360 was inactivated by integrational mutagenesis. Consequently, no alpha-galactosidase activity was detectable in crude extracts of the mutant strain, and it was unable to use melibiose or raffinose as a single carbohydrate source." @default.
- W2116675226 created "2016-06-24" @default.
- W2116675226 creator A5014818908 @default.
- W2116675226 creator A5029452903 @default.
- W2116675226 creator A5060362497 @default.
- W2116675226 creator A5073162505 @default.
- W2116675226 creator A5083262240 @default.
- W2116675226 date "1999-09-01" @default.
- W2116675226 modified "2023-10-16" @default.
- W2116675226 title "Cloning of the Gene Encoding a Novel Thermostable α-Galactosidase from <i>Thermus brockianus</i> ITI360" @default.
- W2116675226 cites W1495076745 @default.
- W2116675226 cites W1577070240 @default.
- W2116675226 cites W1772341111 @default.
- W2116675226 cites W1786727079 @default.
- W2116675226 cites W1805287899 @default.
- W2116675226 cites W1858866489 @default.
- W2116675226 cites W1964479961 @default.
- W2116675226 cites W1965772876 @default.
- W2116675226 cites W1967918409 @default.
- W2116675226 cites W1973241521 @default.
- W2116675226 cites W1975568179 @default.
- W2116675226 cites W1975920595 @default.
- W2116675226 cites W1979388115 @default.
- W2116675226 cites W1983299727 @default.
- W2116675226 cites W1996260512 @default.
- W2116675226 cites W2002677631 @default.
- W2116675226 cites W2006938917 @default.
- W2116675226 cites W2009310436 @default.
- W2116675226 cites W2012514307 @default.
- W2116675226 cites W2018289835 @default.
- W2116675226 cites W2019352025 @default.
- W2116675226 cites W2020755998 @default.
- W2116675226 cites W2028622989 @default.
- W2116675226 cites W2030073277 @default.
- W2116675226 cites W2037214233 @default.
- W2116675226 cites W2044145438 @default.
- W2116675226 cites W2044659933 @default.
- W2116675226 cites W2055043387 @default.
- W2116675226 cites W2068834991 @default.
- W2116675226 cites W2073501728 @default.
- W2116675226 cites W2077455891 @default.
- W2116675226 cites W2077497244 @default.
- W2116675226 cites W2077765784 @default.
- W2116675226 cites W2079689521 @default.
- W2116675226 cites W2083600142 @default.
- W2116675226 cites W2085170068 @default.
- W2116675226 cites W2088301235 @default.
- W2116675226 cites W2092095035 @default.
- W2116675226 cites W2097706568 @default.
- W2116675226 cites W2100837269 @default.
- W2116675226 cites W2106882534 @default.
- W2116675226 cites W2111338604 @default.
- W2116675226 cites W2113255767 @default.
- W2116675226 cites W2113964973 @default.
- W2116675226 cites W2119738543 @default.
- W2116675226 cites W2121875505 @default.
- W2116675226 cites W2128635872 @default.
- W2116675226 cites W2138270253 @default.
- W2116675226 cites W2141210949 @default.
- W2116675226 cites W2152840050 @default.
- W2116675226 cites W2157913345 @default.
- W2116675226 cites W2161524167 @default.
- W2116675226 cites W3155221944 @default.
- W2116675226 cites W4211022623 @default.
- W2116675226 cites W4254327130 @default.
- W2116675226 cites W4293247451 @default.
- W2116675226 doi "https://doi.org/10.1128/aem.65.9.3955-3963.1999" @default.
- W2116675226 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/99726" @default.
- W2116675226 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10473401" @default.
- W2116675226 hasPublicationYear "1999" @default.
- W2116675226 type Work @default.
- W2116675226 sameAs 2116675226 @default.
- W2116675226 citedByCount "71" @default.
- W2116675226 countsByYear W21166752262012 @default.
- W2116675226 countsByYear W21166752262013 @default.
- W2116675226 countsByYear W21166752262014 @default.
- W2116675226 countsByYear W21166752262015 @default.
- W2116675226 countsByYear W21166752262016 @default.
- W2116675226 countsByYear W21166752262017 @default.
- W2116675226 countsByYear W21166752262018 @default.
- W2116675226 countsByYear W21166752262019 @default.
- W2116675226 countsByYear W21166752262020 @default.
- W2116675226 countsByYear W21166752262022 @default.
- W2116675226 countsByYear W21166752262023 @default.
- W2116675226 crossrefType "journal-article" @default.
- W2116675226 hasAuthorship W2116675226A5014818908 @default.
- W2116675226 hasAuthorship W2116675226A5029452903 @default.
- W2116675226 hasAuthorship W2116675226A5060362497 @default.
- W2116675226 hasAuthorship W2116675226A5073162505 @default.
- W2116675226 hasAuthorship W2116675226A5083262240 @default.
- W2116675226 hasBestOaLocation W21166752261 @default.
- W2116675226 hasConcept C104317684 @default.
- W2116675226 hasConcept C142724271 @default.
- W2116675226 hasConcept C167625842 @default.
- W2116675226 hasConcept C181199279 @default.
- W2116675226 hasConcept C19924922 @default.
- W2116675226 hasConcept C2776027139 @default.
- W2116675226 hasConcept C2776788019 @default.