Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116698103> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2116698103 endingPage "1027" @default.
- W2116698103 startingPage "1023" @default.
- W2116698103 abstract "We have previously characterized a protein from Arabidopsis thaliana, called CA-1, that bound to a specific region of the Lhcb1*3 promoter. This binding activity was of interest because the sequence to which it bound is included in a portion of the promoter that is sufficient for phytochrome regulation and because the activity was absent in photomorphogenic mutant det1 seedlings (L. Sun, R.A. Doxsee, E. Harel, E.M. Tobin [1993] Plant Cell 5: 109–121). We have now directly tested whether the nucleotide sequence to which CA-1 binds is required for regulation of the transcription of this gene by phytochrome. A mutation that abolished CA-1 binding in vitro was introduced into a 1.15-kb segment of the Lhcb1*3 promoter, and both the wild-type and mutant promoter fragments were fused to a uidA reporter gene and used to stably transform A. thaliana. Ten different homozygous lines were examined for phytochrome responsiveness for each of the two constructs by assaying [beta]-glucuronidase activity. The wild-type construct showed normal phytochrome responsiveness. The mutant construct showed no phytochrome response, and the overall level of [beta]-glucuronidase activity in etiolated seedlings was decreased by about 2 orders of magnitude. We did not detect a response to a B photoreceptor other than phytochrome itself for either the wild-type or mutant construct. We conclude that information essential for both a high level of expression and phytochrome responsiveness is contained in a 27-bp region to which the CA-1 activity binds." @default.
- W2116698103 created "2016-06-24" @default.
- W2116698103 creator A5033336335 @default.
- W2116698103 creator A5061032046 @default.
- W2116698103 date "1995-07-01" @default.
- W2116698103 modified "2023-09-27" @default.
- W2116698103 title "A Region of the Arabidopsis Lhcb1*3 Promoter That Binds to CA-1 Activity Is Essential for High Expression and Phytochrome Regulation" @default.
- W2116698103 cites W1968615128 @default.
- W2116698103 cites W1975327433 @default.
- W2116698103 cites W1992816799 @default.
- W2116698103 cites W2003425235 @default.
- W2116698103 cites W2008445144 @default.
- W2116698103 cites W2028890005 @default.
- W2116698103 cites W2039154836 @default.
- W2116698103 cites W2048112205 @default.
- W2116698103 cites W2048481226 @default.
- W2116698103 cites W205287634 @default.
- W2116698103 cites W2067363518 @default.
- W2116698103 cites W2079590906 @default.
- W2116698103 cites W2099845265 @default.
- W2116698103 cites W2113212550 @default.
- W2116698103 cites W2136004827 @default.
- W2116698103 cites W2164073443 @default.
- W2116698103 doi "https://doi.org/10.1104/pp.108.3.1023" @default.
- W2116698103 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/157452" @default.
- W2116698103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7630934" @default.
- W2116698103 hasPublicationYear "1995" @default.
- W2116698103 type Work @default.
- W2116698103 sameAs 2116698103 @default.
- W2116698103 citedByCount "29" @default.
- W2116698103 countsByYear W21166981032016 @default.
- W2116698103 countsByYear W21166981032017 @default.
- W2116698103 countsByYear W21166981032018 @default.
- W2116698103 crossrefType "journal-article" @default.
- W2116698103 hasAuthorship W2116698103A5033336335 @default.
- W2116698103 hasAuthorship W2116698103A5061032046 @default.
- W2116698103 hasBestOaLocation W21166981031 @default.
- W2116698103 hasConcept C101762097 @default.
- W2116698103 hasConcept C104317684 @default.
- W2116698103 hasConcept C138885662 @default.
- W2116698103 hasConcept C143065580 @default.
- W2116698103 hasConcept C150194340 @default.
- W2116698103 hasConcept C153911025 @default.
- W2116698103 hasConcept C161733203 @default.
- W2116698103 hasConcept C179926584 @default.
- W2116698103 hasConcept C207583985 @default.
- W2116698103 hasConcept C2775988993 @default.
- W2116698103 hasConcept C2779491563 @default.
- W2116698103 hasConcept C2909544318 @default.
- W2116698103 hasConcept C2986477067 @default.
- W2116698103 hasConcept C41895202 @default.
- W2116698103 hasConcept C55493867 @default.
- W2116698103 hasConcept C59822182 @default.
- W2116698103 hasConcept C86803240 @default.
- W2116698103 hasConcept C90180588 @default.
- W2116698103 hasConcept C95444343 @default.
- W2116698103 hasConceptScore W2116698103C101762097 @default.
- W2116698103 hasConceptScore W2116698103C104317684 @default.
- W2116698103 hasConceptScore W2116698103C138885662 @default.
- W2116698103 hasConceptScore W2116698103C143065580 @default.
- W2116698103 hasConceptScore W2116698103C150194340 @default.
- W2116698103 hasConceptScore W2116698103C153911025 @default.
- W2116698103 hasConceptScore W2116698103C161733203 @default.
- W2116698103 hasConceptScore W2116698103C179926584 @default.
- W2116698103 hasConceptScore W2116698103C207583985 @default.
- W2116698103 hasConceptScore W2116698103C2775988993 @default.
- W2116698103 hasConceptScore W2116698103C2779491563 @default.
- W2116698103 hasConceptScore W2116698103C2909544318 @default.
- W2116698103 hasConceptScore W2116698103C2986477067 @default.
- W2116698103 hasConceptScore W2116698103C41895202 @default.
- W2116698103 hasConceptScore W2116698103C55493867 @default.
- W2116698103 hasConceptScore W2116698103C59822182 @default.
- W2116698103 hasConceptScore W2116698103C86803240 @default.
- W2116698103 hasConceptScore W2116698103C90180588 @default.
- W2116698103 hasConceptScore W2116698103C95444343 @default.
- W2116698103 hasIssue "3" @default.
- W2116698103 hasLocation W21166981031 @default.
- W2116698103 hasLocation W21166981032 @default.
- W2116698103 hasLocation W21166981033 @default.
- W2116698103 hasOpenAccess W2116698103 @default.
- W2116698103 hasPrimaryLocation W21166981031 @default.
- W2116698103 hasRelatedWork W1763052962 @default.
- W2116698103 hasRelatedWork W1964518505 @default.
- W2116698103 hasRelatedWork W2032067966 @default.
- W2116698103 hasRelatedWork W2035848753 @default.
- W2116698103 hasRelatedWork W2127208715 @default.
- W2116698103 hasRelatedWork W2136004827 @default.
- W2116698103 hasRelatedWork W2141626940 @default.
- W2116698103 hasRelatedWork W2165471689 @default.
- W2116698103 hasRelatedWork W2167671547 @default.
- W2116698103 hasRelatedWork W4231401596 @default.
- W2116698103 hasVolume "108" @default.
- W2116698103 isParatext "false" @default.
- W2116698103 isRetracted "false" @default.
- W2116698103 magId "2116698103" @default.
- W2116698103 workType "article" @default.