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- W2116713424 abstract "Plant cell growth depends on a delicate balance between an inner drive—the hydrostatic pressure known as turgor—and an outer restraint—the polymeric wall that surrounds a cell. The classical technique to measure turgor in a single cell, the pressure probe, is intrusive and cannot be applied to small cells. In order to overcome these limitations, we developed a method that combines quantification of topography, nanoindentation force measurements, and an interpretation using a published mechanical model for the pointlike loading of thin elastic shells. We used atomic force microscopy to estimate the elastic properties of the cell wall and turgor pressure from a single force-depth curve. We applied this method to onion epidermal peels and quantified the response to changes in osmolality of the bathing solution. Overall our approach is accessible and enables a straightforward estimation of the hydrostatic pressure inside a walled cell." @default.
- W2116713424 created "2016-06-24" @default.
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- W2116713424 date "2015-05-01" @default.
- W2116713424 modified "2023-10-17" @default.
- W2116713424 title "Quantifying Hydrostatic Pressure in Plant Cells by Using Indentation with an Atomic Force Microscope" @default.
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- W2116713424 doi "https://doi.org/10.1016/j.bpj.2015.03.035" @default.
- W2116713424 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4457008" @default.
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