Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116718796> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2116718796 endingPage "S15" @default.
- W2116718796 startingPage "S15" @default.
- W2116718796 abstract "Fluorescent proteins have been extensively used as protein tags to study the subcellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. However, they have not been frequently used as reporter genes to monitor stimulus-induced gene expression in mammalian cells. Here we demonstrate the use of fluorescent proteins to study stimulus-induced gene transcription. The general applicability of the approach is exemplified by doxycyclin-(Tet-On) and phorbol 12-myristate 13-acetate-induced (c-fos) promoter activation, with green fluorescent protein (GFP) and red fluorescent protein (DsRed) as semiquantitative and immediate reporters, of transcription activation. Under the control of beta-cell-specific promoters, such as the rat insulin 1 promoter or the rat upstream glucokinase promoter, this approach allowed us to monitor online glucose-induced gene transcription in primary beta-cells at the single-cell level as well as in the context of the islet of Langerhans. Applying discretely detectable fluorescent proteins, for example GFP and DsRed, enabled us to simultaneously monitor stimulus-induced transcription by two different promoters in the same cell." @default.
- W2116718796 created "2016-06-24" @default.
- W2116718796 creator A5033200110 @default.
- W2116718796 creator A5037533958 @default.
- W2116718796 creator A5074543896 @default.
- W2116718796 creator A5086295087 @default.
- W2116718796 date "2001-02-01" @default.
- W2116718796 modified "2023-10-03" @default.
- W2116718796 title "Online monitoring of stimulus-induced gene expression in pancreatic beta-cells." @default.
- W2116718796 cites W1490967742 @default.
- W2116718796 cites W1544900705 @default.
- W2116718796 cites W1975876384 @default.
- W2116718796 cites W1980073547 @default.
- W2116718796 cites W1989295391 @default.
- W2116718796 cites W2013459239 @default.
- W2116718796 cites W2025557773 @default.
- W2116718796 cites W2032579724 @default.
- W2116718796 cites W205765424 @default.
- W2116718796 cites W2076964520 @default.
- W2116718796 cites W2091634218 @default.
- W2116718796 cites W2092162739 @default.
- W2116718796 cites W2127068865 @default.
- W2116718796 cites W2160512343 @default.
- W2116718796 doi "https://doi.org/10.2337/diabetes.50.2007.s15" @default.
- W2116718796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11272178" @default.
- W2116718796 hasPublicationYear "2001" @default.
- W2116718796 type Work @default.
- W2116718796 sameAs 2116718796 @default.
- W2116718796 citedByCount "13" @default.
- W2116718796 countsByYear W21167187962012 @default.
- W2116718796 countsByYear W21167187962018 @default.
- W2116718796 crossrefType "journal-article" @default.
- W2116718796 hasAuthorship W2116718796A5033200110 @default.
- W2116718796 hasAuthorship W2116718796A5037533958 @default.
- W2116718796 hasAuthorship W2116718796A5074543896 @default.
- W2116718796 hasAuthorship W2116718796A5086295087 @default.
- W2116718796 hasBestOaLocation W21167187961 @default.
- W2116718796 hasConcept C101762097 @default.
- W2116718796 hasConcept C104317684 @default.
- W2116718796 hasConcept C138885662 @default.
- W2116718796 hasConcept C142613039 @default.
- W2116718796 hasConcept C150194340 @default.
- W2116718796 hasConcept C153911025 @default.
- W2116718796 hasConcept C161733203 @default.
- W2116718796 hasConcept C179926584 @default.
- W2116718796 hasConcept C41895202 @default.
- W2116718796 hasConcept C54009773 @default.
- W2116718796 hasConcept C54355233 @default.
- W2116718796 hasConcept C86339819 @default.
- W2116718796 hasConcept C86803240 @default.
- W2116718796 hasConcept C95444343 @default.
- W2116718796 hasConceptScore W2116718796C101762097 @default.
- W2116718796 hasConceptScore W2116718796C104317684 @default.
- W2116718796 hasConceptScore W2116718796C138885662 @default.
- W2116718796 hasConceptScore W2116718796C142613039 @default.
- W2116718796 hasConceptScore W2116718796C150194340 @default.
- W2116718796 hasConceptScore W2116718796C153911025 @default.
- W2116718796 hasConceptScore W2116718796C161733203 @default.
- W2116718796 hasConceptScore W2116718796C179926584 @default.
- W2116718796 hasConceptScore W2116718796C41895202 @default.
- W2116718796 hasConceptScore W2116718796C54009773 @default.
- W2116718796 hasConceptScore W2116718796C54355233 @default.
- W2116718796 hasConceptScore W2116718796C86339819 @default.
- W2116718796 hasConceptScore W2116718796C86803240 @default.
- W2116718796 hasConceptScore W2116718796C95444343 @default.
- W2116718796 hasIssue "suppl_1" @default.
- W2116718796 hasLocation W21167187961 @default.
- W2116718796 hasLocation W21167187962 @default.
- W2116718796 hasOpenAccess W2116718796 @default.
- W2116718796 hasPrimaryLocation W21167187961 @default.
- W2116718796 hasRelatedWork W1989295391 @default.
- W2116718796 hasRelatedWork W2043166243 @default.
- W2116718796 hasRelatedWork W2046899302 @default.
- W2116718796 hasRelatedWork W2069683015 @default.
- W2116718796 hasRelatedWork W2354883227 @default.
- W2116718796 hasRelatedWork W2363432561 @default.
- W2116718796 hasRelatedWork W2378475372 @default.
- W2116718796 hasRelatedWork W2381695351 @default.
- W2116718796 hasRelatedWork W2389835031 @default.
- W2116718796 hasRelatedWork W2413743288 @default.
- W2116718796 hasVolume "50" @default.
- W2116718796 isParatext "false" @default.
- W2116718796 isRetracted "false" @default.
- W2116718796 magId "2116718796" @default.
- W2116718796 workType "article" @default.