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• W2116720521 abstract "The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration." @default.
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• W2116720521 date "2010-01-01" @default.
• W2116720521 modified "2023-10-16" @default.
• W2116720521 title "Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy" @default.
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• W2116720521 doi "https://doi.org/10.1117/1.3475954" @default.
• W2116720521 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2937046" @default.
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