Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116724414> ?p ?o ?g. }
- W2116724414 endingPage "3517" @default.
- W2116724414 startingPage "3507" @default.
- W2116724414 abstract "ABSTRACT Hepatitis B e antigen (HBeAg) is a secreted version of hepatitis B virus (HBV) core protein that promotes immune tolerance and persistent infection. It is derived from a translation product of the precore/core gene by two proteolytic cleavage events: removal of the amino-terminal signal peptide and removal of the carboxyl-terminal arginine-rich sequence. Four RXXR motifs are present at the carboxyl terminus of the HBeAg precursor, with the first two fused as 151 RRGRSPR 157 . Genotype A possesses two extra amino acids at the first motif ( 151 RRDRGRSPR 159 ), which weakens the first motif and separates it from the second one. Western blot analysis of patient sera revealed a single HBeAg form for genotypes B to D but two additional forms of larger sizes for genotype A. Site-directed mutagenesis and transfection experiments with human hepatoma cell lines indicated that HBeAg of genotype B is derived from cleavage at the first ( 151 RRGR 154 ) motif. The major HBeAg form of genotype A corresponds to cleavage at the second ( 156 RSPR 159 ) motif, and the other two forms are cleavage products of the first ( 151 RRDR 154 ) and third ( 166 RRRR 169 ) motifs, respectively. Only the cleavage product of the third motif of genotype A was observed in furin-deficient LoVo cells, and an inhibitor of furin-like proprotein convertases blocked cleavage of the first and second motifs in human hepatoma cells. In conclusion, our study reveals genotypic differences in HBeAg processing and implicates furin as the major enzyme involved in the cleavage of the first and second RXXR motifs." @default.
- W2116724414 created "2016-06-24" @default.
- W2116724414 creator A5010854797 @default.
- W2116724414 creator A5013290027 @default.
- W2116724414 creator A5051099030 @default.
- W2116724414 creator A5063396100 @default.
- W2116724414 date "2009-04-15" @default.
- W2116724414 modified "2023-10-03" @default.
- W2116724414 title "Characterization of Genotype-Specific Carboxyl-Terminal Cleavage Sites of Hepatitis B Virus e Antigen Precursor and Identification of Furin as the Candidate Enzyme" @default.
- W2116724414 cites W1501417543 @default.
- W2116724414 cites W1549912128 @default.
- W2116724414 cites W1556594156 @default.
- W2116724414 cites W1569611860 @default.
- W2116724414 cites W1572444605 @default.
- W2116724414 cites W1611263718 @default.
- W2116724414 cites W1624197021 @default.
- W2116724414 cites W1749453950 @default.
- W2116724414 cites W1762842652 @default.
- W2116724414 cites W1799328282 @default.
- W2116724414 cites W1813446025 @default.
- W2116724414 cites W1872095343 @default.
- W2116724414 cites W1885047062 @default.
- W2116724414 cites W1894278338 @default.
- W2116724414 cites W1894295618 @default.
- W2116724414 cites W1922003631 @default.
- W2116724414 cites W1939693655 @default.
- W2116724414 cites W1949173713 @default.
- W2116724414 cites W1951551030 @default.
- W2116724414 cites W1963623444 @default.
- W2116724414 cites W1963875503 @default.
- W2116724414 cites W1968913867 @default.
- W2116724414 cites W1978551768 @default.
- W2116724414 cites W1978656930 @default.
- W2116724414 cites W1988373760 @default.
- W2116724414 cites W1990209576 @default.
- W2116724414 cites W1991576220 @default.
- W2116724414 cites W1992887655 @default.
- W2116724414 cites W2007925721 @default.
- W2116724414 cites W2008517556 @default.
- W2116724414 cites W2009029675 @default.
- W2116724414 cites W2010621350 @default.
- W2116724414 cites W2016648335 @default.
- W2116724414 cites W2018479084 @default.
- W2116724414 cites W2034679908 @default.
- W2116724414 cites W2041440407 @default.
- W2116724414 cites W2041626675 @default.
- W2116724414 cites W2044296969 @default.
- W2116724414 cites W2044565887 @default.
- W2116724414 cites W2046196885 @default.
- W2116724414 cites W2046956651 @default.
- W2116724414 cites W2049850687 @default.
- W2116724414 cites W2056947674 @default.
- W2116724414 cites W2067183161 @default.
- W2116724414 cites W2070733082 @default.
- W2116724414 cites W2081556170 @default.
- W2116724414 cites W2082920591 @default.
- W2116724414 cites W2096212331 @default.
- W2116724414 cites W2097018453 @default.
- W2116724414 cites W2097812689 @default.
- W2116724414 cites W2101032357 @default.
- W2116724414 cites W2128941790 @default.
- W2116724414 cites W2129914448 @default.
- W2116724414 cites W2134562820 @default.
- W2116724414 cites W2135201594 @default.
- W2116724414 cites W2139858623 @default.
- W2116724414 cites W2140382373 @default.
- W2116724414 cites W2141044770 @default.
- W2116724414 cites W2144305684 @default.
- W2116724414 cites W2146867161 @default.
- W2116724414 cites W2166720212 @default.
- W2116724414 cites W2171473776 @default.
- W2116724414 cites W2327856202 @default.
- W2116724414 cites W2416878139 @default.
- W2116724414 cites W4253422011 @default.
- W2116724414 doi "https://doi.org/10.1128/jvi.02348-08" @default.
- W2116724414 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2663268" @default.
- W2116724414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19193799" @default.
- W2116724414 hasPublicationYear "2009" @default.
- W2116724414 type Work @default.
- W2116724414 sameAs 2116724414 @default.
- W2116724414 citedByCount "75" @default.
- W2116724414 countsByYear W21167244142012 @default.
- W2116724414 countsByYear W21167244142013 @default.
- W2116724414 countsByYear W21167244142014 @default.
- W2116724414 countsByYear W21167244142015 @default.
- W2116724414 countsByYear W21167244142016 @default.
- W2116724414 countsByYear W21167244142017 @default.
- W2116724414 countsByYear W21167244142018 @default.
- W2116724414 countsByYear W21167244142019 @default.
- W2116724414 countsByYear W21167244142020 @default.
- W2116724414 countsByYear W21167244142021 @default.
- W2116724414 countsByYear W21167244142022 @default.
- W2116724414 countsByYear W21167244142023 @default.
- W2116724414 crossrefType "journal-article" @default.
- W2116724414 hasAuthorship W2116724414A5010854797 @default.
- W2116724414 hasAuthorship W2116724414A5013290027 @default.
- W2116724414 hasAuthorship W2116724414A5051099030 @default.
- W2116724414 hasAuthorship W2116724414A5063396100 @default.