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• W2116726186 abstract "Class II HLA-DR genes play an important role in the immune response to viral antigens. The effect of measles vaccine virus (MVV) infection on the induction of self-peptides presented by HLA-DR molecules during the immune response to viral infection is poorly known. Here, we describe a strategy for isolation and rapid sequence determination of an MVV-inducible class II bound peptide from a membrane protein (Leu-13). Peptides bound to HLA-DR4 (DRB1*0401 peptide complex) were eluted from immunoaffinity-purified HLA-DR4, peptides were differentially screened by MALDI-TOF-MS and subsequently sequenced by post source decay (PSD)-MALDI-TOF-MS. Human B-cells infected with MVV demonstrated an enhanced pattern of self-peptide production after MVV infection. This relatively simple analytical protocol provides a sensitive method for the direct identification of peptides associated with MHC class II DR molecules. More broadly, this same approach can be used to identify sequences of specific MVV processed peptides presented by any class II MHC DR molecule." @default.
• W2116726186 created "2016-06-24" @default.
• W2116726186 creator A5018582122 @default.
• W2116726186 creator A5054301416 @default.
• W2116726186 creator A5057546038 @default.
• W2116726186 creator A5063582082 @default.
• W2116726186 date "2000-12-01" @default.
• W2116726186 modified "2023-10-10" @default.
• W2116726186 title "Isolation and rapid identification of an abundant self-peptide from class II HLA-DRB1*0401 alleles induced by measles vaccine virus infection" @default.
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• W2116726186 doi "https://doi.org/10.1016/s0022-1759(00)00278-7" @default.
• W2116726186 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11121542" @default.
• W2116726186 hasPublicationYear "2000" @default.
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