FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116727056> ?p ?o ?g. }

• W2116727056 endingPage "158" @default.
• W2116727056 startingPage "151" @default.
• W2116727056 abstract "Tomato spotted wilt virus (TSWV) is an economically important viral pathogen of a wide range of field and horticultural crops. We developed an artificial microRNA (amiRNA) strategy against TSWV, targeting the nucleoprotein (N) and silencing suppressor (NSs) genes. The amiRNA constructs replaced the natural miRNA in a shortened Arabidopsis 173-nucleotide (nt) miR159a precursor backbone (athmiR159a) without the stem base extending beyond the miR/miR* duplex. Further, each amiRNA was modified to contain a mismatch (wobble) sequence at nucleotide position 12 and 13 on the complementary strand amiRNA*, mimicking the endogenous miR159a sequence structure. Transient expression in Nicotiana benthamiana demonstrated that the introduction of a wobble sequence did not alter amiRNA expression levels. Following challenge inoculation with TSWV, plants expressing N-specific amiRNAs with or without the wobble remained asymptomatic and were negative for TSWV by ELISA. In contrast, plants expressing the NSs-specific amiRNAs were symptomatic and accumulated high levels of TSWV. Similar findings were obtained in stably transformed Nicotiana tabacum plants. Our results show that a shortened 173-nt athmiR159a backbone is sufficient to express amiRNAs and that the presence of mismatch at position 12-13 does not influence amiRNA expression or conferring of resistance. We also show that selection of target gene and positional effect are critical in amiRNA-based approach for introducing resistance. These findings open the possibility of employing the amiRNA approach for broad-spectrum resistance to tospoviruses as well as other viruses." @default.
• W2116727056 created "2016-06-24" @default.
• W2116727056 creator A5029494855 @default.
• W2116727056 creator A5058032963 @default.
• W2116727056 creator A5065660680 @default.
• W2116727056 creator A5068572251 @default.
• W2116727056 creator A5077618903 @default.
• W2116727056 creator A5086257407 @default.
• W2116727056 creator A5089080093 @default.
• W2116727056 creator A5025988037 @default.
• W2116727056 date "2016-01-01" @default.
• W2116727056 modified "2023-10-12" @default.
• W2116727056 title "Evaluation and identification of candidate genes for artificial microRNA-mediated resistance to tomato spotted wilt virus" @default.
• W2116727056 cites W144423133 @default.
• W2116727056 cites W1606490089 @default.
• W2116727056 cites W1611262789 @default.
• W2116727056 cites W1677940699 @default.
• W2116727056 cites W1953462468 @default.
• W2116727056 cites W1967615187 @default.
• W2116727056 cites W1975204036 @default.
• W2116727056 cites W1979006998 @default.
• W2116727056 cites W1992842489 @default.
• W2116727056 cites W1994273846 @default.
• W2116727056 cites W1997562450 @default.
• W2116727056 cites W1998394718 @default.
• W2116727056 cites W2001365069 @default.
• W2116727056 cites W2006190250 @default.
• W2116727056 cites W2014004435 @default.
• W2116727056 cites W2016022129 @default.
• W2116727056 cites W2028903821 @default.
• W2116727056 cites W2029367671 @default.
• W2116727056 cites W2031715440 @default.
• W2116727056 cites W2033049792 @default.
• W2116727056 cites W2044002382 @default.
• W2116727056 cites W2048078120 @default.
• W2116727056 cites W2066133957 @default.
• W2116727056 cites W2067087372 @default.
• W2116727056 cites W2070763881 @default.
• W2116727056 cites W2071596792 @default.
• W2116727056 cites W2073991021 @default.
• W2116727056 cites W2078293094 @default.
• W2116727056 cites W2081452698 @default.
• W2116727056 cites W2082213106 @default.
• W2116727056 cites W2082315179 @default.
• W2116727056 cites W2084058548 @default.
• W2116727056 cites W2085178658 @default.
• W2116727056 cites W2088841098 @default.
• W2116727056 cites W2107188967 @default.
• W2116727056 cites W2111037262 @default.
• W2116727056 cites W2112167962 @default.
• W2116727056 cites W2118911862 @default.
• W2116727056 cites W2134418609 @default.
• W2116727056 cites W2135015580 @default.
• W2116727056 cites W2135655483 @default.
• W2116727056 cites W2145043911 @default.
• W2116727056 cites W2149221950 @default.
• W2116727056 cites W2151528904 @default.
• W2116727056 cites W2152788729 @default.
• W2116727056 cites W2153598186 @default.
• W2116727056 cites W2156401301 @default.
• W2116727056 cites W2160629953 @default.
• W2116727056 cites W2162170048 @default.
• W2116727056 cites W2163490283 @default.
• W2116727056 cites W2163742432 @default.
• W2116727056 cites W2168053136 @default.
• W2116727056 cites W2170934154 @default.
• W2116727056 cites W2335144176 @default.
• W2116727056 doi "https://doi.org/10.1016/j.virusres.2015.10.003" @default.
• W2116727056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26454192" @default.
• W2116727056 hasPublicationYear "2016" @default.
• W2116727056 type Work @default.
• W2116727056 sameAs 2116727056 @default.
• W2116727056 citedByCount "32" @default.
• W2116727056 countsByYear W21167270562016 @default.
• W2116727056 countsByYear W21167270562017 @default.
• W2116727056 countsByYear W21167270562018 @default.
• W2116727056 countsByYear W21167270562019 @default.
• W2116727056 countsByYear W21167270562020 @default.
• W2116727056 countsByYear W21167270562021 @default.
• W2116727056 countsByYear W21167270562022 @default.
• W2116727056 crossrefType "journal-article" @default.
• W2116727056 hasAuthorship W2116727056A5025988037 @default.
• W2116727056 hasAuthorship W2116727056A5029494855 @default.
• W2116727056 hasAuthorship W2116727056A5058032963 @default.
• W2116727056 hasAuthorship W2116727056A5065660680 @default.
• W2116727056 hasAuthorship W2116727056A5068572251 @default.
• W2116727056 hasAuthorship W2116727056A5077618903 @default.
• W2116727056 hasAuthorship W2116727056A5086257407 @default.
• W2116727056 hasAuthorship W2116727056A5089080093 @default.
• W2116727056 hasConcept C102230213 @default.
• W2116727056 hasConcept C104317684 @default.
• W2116727056 hasConcept C109110057 @default.
• W2116727056 hasConcept C119056186 @default.
• W2116727056 hasConcept C122172344 @default.
• W2116727056 hasConcept C145059251 @default.
• W2116727056 hasConcept C159047783 @default.
• W2116727056 hasConcept C166703698 @default.
• W2116727056 hasConcept C2522874641 @default.

• next