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• W2116732031 abstract "A simple enzymatic labeling procedure is described to determine spot quality in oligonucleotide microarrays. By using fluorescently labeled dideoxynucleotides or ribonucleotides as substrate for terminal deoxynucleotidyl transferase (TdT), a single fluorophore can be covalently attached at the 3′ end of each oligonucleotide probe molecule in the spot. Fluorescein- 12-ddUTP, Cy™3-ddUTP, Cy5-UTP, and Cy3-UTP were compared as TdT substrates for 3′ end labeling an array of 1273 hexamer probes. Cy5-UTP was found to show minimal bias toward probe base composition and is therefore well suited for quantitative analysis of microarray spots where the oligonucleotide probes are coupled via a 5′ end linkage to the solid phase." @default.
• W2116732031 created "2016-06-24" @default.
• W2116732031 creator A5087691382 @default.
• W2116732031 date "2006-07-01" @default.
• W2116732031 modified "2023-10-10" @default.
• W2116732031 title "Analysis of oligonucleotide microarrays by 3<b>′</b> end labeling using fluorescent nucleotides and terminal transferase" @default.
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• W2116732031 doi "https://doi.org/10.2144/000112182" @default.
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