Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116738410> ?p ?o ?g. }
- W2116738410 endingPage "1042" @default.
- W2116738410 startingPage "1029" @default.
- W2116738410 abstract "Abstract Most human γδ T cells express Vγ2Vδ2 TCRs and play important roles in microbial and tumor immunity. Vγ2Vδ2 T cells are stimulated by self- and foreign prenyl pyrophosphate intermediates in isoprenoid synthesis. However, little is known about the molecular basis for this stimulation. We find that a mAb specific for butyrophilin 3 (BTN3)/CD277 Ig superfamily proteins mimics prenyl pyrophosphates. The 20.1 mAb stimulated Vγ2Vδ2 T cell clones regardless of their functional phenotype or developmental origin and selectively expanded blood Vγ2Vδ2 T cells. The γδ TCR mediates 20.1 mAb stimulation because IL-2 is released by β− Jurkat cells transfected with Vγ2Vδ2 TCRs. 20.1 stimulation was not due to isopentenyl pyrophosphate (IPP) accumulation because 20.1 treatment of APC did not increase IPP levels. In addition, stimulation was not inhibited by statin treatment, which blocks IPP production. Importantly, small interfering RNA knockdown of BTN3A1 abolished stimulation by IPP that could be restored by re-expression of BTN3A1 but not by BTN3A2 or BTN3A3. Rhesus monkey and baboon APC presented HMBPP and 20.1 to human Vγ2Vδ2 T cells despite amino acid differences in BTN3A1 that localize to its outer surface. This suggests that the conserved inner and/or top surfaces of BTN3A1 interact with its counterreceptor. Although no binding site exists on the BTN3A1 extracellular domains, a model of the intracellular B30.2 domain predicts a basic pocket on its binding surface. However, BTN3A1 did not preferentially bind a photoaffinity prenyl pyrophosphate. Thus, BTN3A1 is required for stimulation by prenyl pyrophosphates but does not bind the intermediates with high affinity." @default.
- W2116738410 created "2016-06-24" @default.
- W2116738410 creator A5000959360 @default.
- W2116738410 creator A5004120095 @default.
- W2116738410 creator A5020637669 @default.
- W2116738410 creator A5033020983 @default.
- W2116738410 creator A5035735834 @default.
- W2116738410 creator A5047918872 @default.
- W2116738410 creator A5048332389 @default.
- W2116738410 creator A5086521398 @default.
- W2116738410 date "2013-08-01" @default.
- W2116738410 modified "2023-09-30" @default.
- W2116738410 title "Butyrophilin 3A1 Plays an Essential Role in Prenyl Pyrophosphate Stimulation of Human Vγ2Vδ2 T Cells" @default.
- W2116738410 cites W1506942171 @default.
- W2116738410 cites W1544076059 @default.
- W2116738410 cites W1567929518 @default.
- W2116738410 cites W1574496133 @default.
- W2116738410 cites W1594381616 @default.
- W2116738410 cites W1650924090 @default.
- W2116738410 cites W1809672664 @default.
- W2116738410 cites W1895004653 @default.
- W2116738410 cites W1899488625 @default.
- W2116738410 cites W1936790111 @default.
- W2116738410 cites W1954884893 @default.
- W2116738410 cites W1963372189 @default.
- W2116738410 cites W1992854238 @default.
- W2116738410 cites W1995968109 @default.
- W2116738410 cites W2001665937 @default.
- W2116738410 cites W2002147164 @default.
- W2116738410 cites W2003797329 @default.
- W2116738410 cites W2005323388 @default.
- W2116738410 cites W2006957104 @default.
- W2116738410 cites W2010361151 @default.
- W2116738410 cites W2013505681 @default.
- W2116738410 cites W2015641127 @default.
- W2116738410 cites W2017453418 @default.
- W2116738410 cites W2018540827 @default.
- W2116738410 cites W2022754521 @default.
- W2116738410 cites W2024562564 @default.
- W2116738410 cites W2026391181 @default.
- W2116738410 cites W2029392258 @default.
- W2116738410 cites W2029582401 @default.
- W2116738410 cites W2053449731 @default.
- W2116738410 cites W2055957092 @default.
- W2116738410 cites W2060141919 @default.
- W2116738410 cites W2060588878 @default.
- W2116738410 cites W2061057762 @default.
- W2116738410 cites W2061699947 @default.
- W2116738410 cites W2065379975 @default.
- W2116738410 cites W2070095951 @default.
- W2116738410 cites W2073312795 @default.
- W2116738410 cites W2073334951 @default.
- W2116738410 cites W2074671087 @default.
- W2116738410 cites W2083601518 @default.
- W2116738410 cites W2083873060 @default.
- W2116738410 cites W2094207989 @default.
- W2116738410 cites W2098528749 @default.
- W2116738410 cites W2100773611 @default.
- W2116738410 cites W2100915708 @default.
- W2116738410 cites W2108499896 @default.
- W2116738410 cites W2111828455 @default.
- W2116738410 cites W2113358352 @default.
- W2116738410 cites W2113656623 @default.
- W2116738410 cites W2114859237 @default.
- W2116738410 cites W2117118487 @default.
- W2116738410 cites W2118324893 @default.
- W2116738410 cites W2125994015 @default.
- W2116738410 cites W2127367385 @default.
- W2116738410 cites W2127900736 @default.
- W2116738410 cites W2129035692 @default.
- W2116738410 cites W2129799990 @default.
- W2116738410 cites W2130967257 @default.
- W2116738410 cites W2133289974 @default.
- W2116738410 cites W2133649859 @default.
- W2116738410 cites W2135099287 @default.
- W2116738410 cites W2141531733 @default.
- W2116738410 cites W2141989396 @default.
- W2116738410 cites W2142565339 @default.
- W2116738410 cites W2142660310 @default.
- W2116738410 cites W2145019485 @default.
- W2116738410 cites W2148532595 @default.
- W2116738410 cites W2150876158 @default.
- W2116738410 cites W2153901715 @default.
- W2116738410 cites W2156355362 @default.
- W2116738410 cites W2158524503 @default.
- W2116738410 cites W2161251896 @default.
- W2116738410 cites W2163819348 @default.
- W2116738410 cites W2165565773 @default.
- W2116738410 cites W2170263258 @default.
- W2116738410 doi "https://doi.org/10.4049/jimmunol.1300658" @default.
- W2116738410 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3884521" @default.
- W2116738410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23833237" @default.
- W2116738410 hasPublicationYear "2013" @default.
- W2116738410 type Work @default.
- W2116738410 sameAs 2116738410 @default.
- W2116738410 citedByCount "138" @default.
- W2116738410 countsByYear W21167384102013 @default.
- W2116738410 countsByYear W21167384102014 @default.