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• W2116740563 abstract "We describe a new simple solid-phase competitive luminescence immunoassay (LIA) for the determination of immunoglobulin A (IgA) in faeces. The assay utilizes an anti-α-chain IgA antibody which is coated to polystyrene beads and acridinium ester-labelled human IgA as tracer and, therefore, measures both monomeric and polymeric IgA. Dilution recovery of an internal standard was 96, 100 and 103%. Interassay and intra-assay coefficients of variation (C.V.) ranged from 4.5 to 12.9%. The upper limit of normal of faecal IgA in 122 healthy controls was found to be 300 mg/1 IgA (mean 73 mg/1, specificity of 99.2%). Patients with inactive Crohn's disease (Crohn's disease activity index (CDAI < 150, n = 14) had faecal IgA values up to 3317 mg/1 (mean 1073 mg/1; P < 0.0001). In the active group (CDAI > 150, n = 26) faecal IgA values ranged from 49 to 4094 mg/1 (mean 1253 mg/1; P < 0.0001). Patients with ulcerative colitis were divided into a group with active disease (n = 18) and a remission group (n = 16) with values up to 1843 mg/1 faecal IgA (mean 486 mg/1; P < 0.0032) and up to 602 mg/1 faecal IgA (mean 176 mg/1; P < 0.4833), respectively. We also studied patients with non-inflammatory diseases of the gut with this assay. This LIA has proved to be a reliable method for the determination of elevated faecal IgA concentrations and for the detection of pathological findings in the gastrointestinal tract, especially in Crohn's disease." @default.
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• W2116740563 date "1997-05-01" @default.
• W2116740563 modified "2023-09-26" @default.
• W2116740563 title "Solid phase competitive luminescence immunoassay for immunoglobulin A in faeces: development and clinical validation" @default.
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• W2116740563 doi "https://doi.org/10.1016/s0009-8981(96)06510-2" @default.
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