FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116773242> ?p ?o ?g. }

• W2116773242 endingPage "15748" @default.
• W2116773242 startingPage "15739" @default.
• W2116773242 abstract "The activation of coagulation factors V and X by Russell's viper venom (RVV) has been implicated in the development of consumptive coagulopathies in severely envenomed patients. However, factor Va is prone to inactivation by activated protein C (APC), an important serine protease that negatively regulates blood coagulation. It is therefore hypothesized that APC may be down-regulated by some of the venom components. In this study, we managed to isolate a potent Kunitz-type APC inhibitor, named DrKIn-I. Using chromogenic substrate, DrKIn-I dose-dependently inhibited the activity of APC. Heparin potentiated the inhibition and reduced the IC(50) of DrKIn-I by 25-fold. DrKIn-I, together with heparin, also protected factor Va from APC-mediated inactivation. Using surface plasmon resonance, DrKIn-I exhibited fast binding kinetics with APC (association rate constant = 1.7 × 10(7) M(-1) s(-1)). Direct binding assays and kinetic studies revealed that this inhibition (K(i) = 53 pM) is due to the tight binding interactions of DrKIn-I with both heparin and APC. DrKIn-I also effectively reversed the anticoagulant activity of APC and completely restored the thrombin generation in APC-containing plasma. Furthermore, although the injection of either DrKIn-I or RVV-X (the venom factor X-activator) into ICR mice did not significantly deplete the plasma fibrinogen concentration, co-administration of DrKIn-I with RVV-X resulted in complete fibrinogen consumption and the deposition of fibrin thrombi in the glomerular capillaries. Our results provide new insights into the pathogenesis of RVV-induced coagulopathies and indicate that DrKIn-I is a novel APC inhibitor that is associated with potentially fatal thrombotic complications in Russell's viper envenomation." @default.
• W2116773242 created "2016-06-24" @default.
• W2116773242 creator A5048858926 @default.
• W2116773242 creator A5066084120 @default.
• W2116773242 creator A5068229730 @default.
• W2116773242 creator A5079365183 @default.
• W2116773242 date "2012-05-01" @default.
• W2116773242 modified "2023-10-18" @default.
• W2116773242 title "A Novel Heparin-dependent Inhibitor of Activated Protein C That Potentiates Consumptive Coagulopathy in Russell's Viper Envenomation" @default.
• W2116773242 cites W1591272093 @default.
• W2116773242 cites W1827982969 @default.
• W2116773242 cites W184609806 @default.
• W2116773242 cites W196422501 @default.
• W2116773242 cites W1967496809 @default.
• W2116773242 cites W1971350777 @default.
• W2116773242 cites W1971943559 @default.
• W2116773242 cites W1972582318 @default.
• W2116773242 cites W1972900899 @default.
• W2116773242 cites W1980659802 @default.
• W2116773242 cites W2009287427 @default.
• W2116773242 cites W2015110474 @default.
• W2116773242 cites W2021412008 @default.
• W2116773242 cites W2028978518 @default.
• W2116773242 cites W2029319561 @default.
• W2116773242 cites W2032049968 @default.
• W2116773242 cites W2034665579 @default.
• W2116773242 cites W2035823060 @default.
• W2116773242 cites W2041726150 @default.
• W2116773242 cites W2041833840 @default.
• W2116773242 cites W2046785394 @default.
• W2116773242 cites W2055883779 @default.
• W2116773242 cites W2060466212 @default.
• W2116773242 cites W2061440506 @default.
• W2116773242 cites W2065375178 @default.
• W2116773242 cites W2068519554 @default.
• W2116773242 cites W2069484435 @default.
• W2116773242 cites W2081489602 @default.
• W2116773242 cites W2092751827 @default.
• W2116773242 cites W2094393536 @default.
• W2116773242 cites W2094605213 @default.
• W2116773242 cites W2103197287 @default.
• W2116773242 cites W2112348962 @default.
• W2116773242 cites W2118405368 @default.
• W2116773242 cites W2118780215 @default.
• W2116773242 cites W2136453624 @default.
• W2116773242 cites W2137001905 @default.
• W2116773242 cites W2137073015 @default.
• W2116773242 cites W2138654983 @default.
• W2116773242 cites W2162795321 @default.
• W2116773242 cites W2169475430 @default.
• W2116773242 cites W2239897670 @default.
• W2116773242 cites W2300798205 @default.
• W2116773242 cites W2884197938 @default.
• W2116773242 cites W30895601 @default.
• W2116773242 cites W4313376947 @default.
• W2116773242 doi "https://doi.org/10.1074/jbc.m111.323063" @default.
• W2116773242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3346151" @default.
• W2116773242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22416129" @default.
• W2116773242 hasPublicationYear "2012" @default.
• W2116773242 type Work @default.
• W2116773242 sameAs 2116773242 @default.
• W2116773242 citedByCount "17" @default.
• W2116773242 countsByYear W21167732422013 @default.
• W2116773242 countsByYear W21167732422014 @default.
• W2116773242 countsByYear W21167732422015 @default.
• W2116773242 countsByYear W21167732422016 @default.
• W2116773242 countsByYear W21167732422017 @default.
• W2116773242 countsByYear W21167732422018 @default.
• W2116773242 countsByYear W21167732422019 @default.
• W2116773242 countsByYear W21167732422023 @default.
• W2116773242 crossrefType "journal-article" @default.
• W2116773242 hasAuthorship W2116773242A5048858926 @default.
• W2116773242 hasAuthorship W2116773242A5066084120 @default.
• W2116773242 hasAuthorship W2116773242A5068229730 @default.
• W2116773242 hasAuthorship W2116773242A5079365183 @default.
• W2116773242 hasBestOaLocation W21167732421 @default.
• W2116773242 hasConcept C126322002 @default.
• W2116773242 hasConcept C153911025 @default.
• W2116773242 hasConcept C181199279 @default.
• W2116773242 hasConcept C185592680 @default.
• W2116773242 hasConcept C203014093 @default.
• W2116773242 hasConcept C2776714187 @default.
• W2116773242 hasConcept C2776725428 @default.
• W2116773242 hasConcept C2776816377 @default.
• W2116773242 hasConcept C2777292125 @default.
• W2116773242 hasConcept C2777557582 @default.
• W2116773242 hasConcept C2777807008 @default.
• W2116773242 hasConcept C2778382381 @default.
• W2116773242 hasConcept C2779036427 @default.
• W2116773242 hasConcept C2779448229 @default.
• W2116773242 hasConcept C2781287897 @default.
• W2116773242 hasConcept C2910624479 @default.
• W2116773242 hasConcept C54173615 @default.
• W2116773242 hasConcept C55493867 @default.
• W2116773242 hasConcept C71924100 @default.
• W2116773242 hasConcept C86803240 @default.
• W2116773242 hasConcept C89560881 @default.
• W2116773242 hasConcept C98274493 @default.

• next