FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116775017> ?p ?o ?g. }

• W2116775017 endingPage "893" @default.
• W2116775017 startingPage "892" @default.
• W2116775017 abstract "EDITOR: Toll-like receptor 4 (TLR4), a receptor of innate immunity, provides the site for the lipopolysaccharide shell of Gram-negative organisms to bind and initiate the inflammatory cascade in sepsis. Its expression and polymorphisms (Asp299Gly and Thr399lle) have been studied in various disease states (sepsis, asthma, atherosclerosis, immunodeficiencies), ethnic groups, ages, levels of physical training, gestational status and trauma [1–7]. The expanding body of evidence in regard to TLR signalling is demonstrating the importance between such signalling and human disease. A translational research project between the Departments of Anaesthesiology and Medical Microbiology and Immunology was initiated to determine any difference in the amount of TLR4 expression in elective preoperative patients in regard to height, weight, age, gender, body surface area, body mass index, hypertension and diabetes. Race was not analysed. Case series After institutional research board approval a prospective study of the blood specimens of 52 consecutive elective, preoperative patients was performed. The blood was directly decanted into a PAXgene Blood RNA tube (Qiagen) designed for the stabilization of RNA from whole blood. Whole blood RNA was extracted using the PAXgenez™ Blood RNA System (PreAnalytiX, Hombrechitikon, CH), including treatment with DNase I to prevent genomic DNA contamination (Qiagen). Extracted RNA was stored at 80°C until required for copy of DNA (cDNA) synthesis. Quantification of TLR4 expression levels in human lymphocytes was by real time polymerase chain reaction (RT-PCR) and results compared to standard curves. Pearsons's correlation coefficient was used for statistical analysis of the continuous variables and t-test was used in the analysis of the categorical variables (see Tables 1 and 2). The TLR4 concentration varied from 0.211 to 2.490 fmol μL−1 per cDNA, however there was no statistical significance between the concentration of TLR4/cDNA expressed by RT-PCR and the continuous variables: height, weight, body mass index or surface area and age, nor the categorical variables: hypertension, diabetes and gender. None of the patients had postoperative infections upon follow-up a week later.Table 1: Continuous variables.Table 2: Categorical variables.Discussion This study was an initial attempt to quantify the TLR4 receptor expression by elective patient variable preoperatively. While we found no variation of TLR4 in our elective preoperative patients we are cognizant of several limiting factors in our pilot study: (1) the small size of our cohort introduces the possibility of a Type II error, (2) the Asp299Gly and Thr399lle TLR4 polymorphisms were not addressed and (3) race was not evaluated. However, we plan to extend our epidemiological investigation of TLR4 in the perioperative setting because it would be fascinating to determine if the expression of TLR4 and its quantitative determination are similar in all humans regardless of physical characteristics (polymorphisms included). It is noteworthy that a 10-fold variability in the concentration of TLR4/cDNA was not of statistical significance in this study thus raising an interesting scientific interrogative as to the biological significance of this 10-fold difference in regard to innate body defences if an infection were to become established. This matter remains to be investigated. Understanding how TLR4 receptors influence the human immune response and how the lipopolysaccharide of Gram-negative bacteria evades defences may allow future physicians and scientists to alter or manipulate TLR4 activation in order to provide for improved postoperative outcomes. Additionally, placing biological results aside, this endeavour allowed bench researchers and clinicians to engage in a collaborative, translational relationship that provided an opportunity for anaesthesiologists to take their practice to the laboratory and to potentially explore the immunological basis for their actions. T. J. Papadimos L. Smith S. Mukherjee D. Popovic L. Y. Chen Z. K. Pan 1Department of Anesthesiology, Immunology and Medical Microbiology, Medical University of Ohio, Toledo, OH, USA" @default.
• W2116775017 created "2016-06-24" @default.
• W2116775017 creator A5016734479 @default.
• W2116775017 creator A5022200161 @default.
• W2116775017 creator A5046553881 @default.
• W2116775017 creator A5056735500 @default.
• W2116775017 creator A5083876739 @default.
• W2116775017 creator A5088132020 @default.
• W2116775017 date "2006-10-01" @default.
• W2116775017 modified "2023-09-25" @default.
• W2116775017 title "A lack of toll-like receptor 4 expression variability in the immediate preoperative period" @default.
• W2116775017 cites W1997051283 @default.
• W2116775017 cites W2001883479 @default.
• W2116775017 cites W2005423595 @default.
• W2116775017 cites W2008003223 @default.
• W2116775017 cites W2083945275 @default.
• W2116775017 cites W2160243792 @default.
• W2116775017 cites W2328185249 @default.
• W2116775017 doi "https://doi.org/10.1017/s0265021506221379" @default.
• W2116775017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16953946" @default.
• W2116775017 hasPublicationYear "2006" @default.
• W2116775017 type Work @default.
• W2116775017 sameAs 2116775017 @default.
• W2116775017 citedByCount "0" @default.
• W2116775017 crossrefType "journal-article" @default.
• W2116775017 hasAuthorship W2116775017A5016734479 @default.
• W2116775017 hasAuthorship W2116775017A5022200161 @default.
• W2116775017 hasAuthorship W2116775017A5046553881 @default.
• W2116775017 hasAuthorship W2116775017A5056735500 @default.
• W2116775017 hasAuthorship W2116775017A5083876739 @default.
• W2116775017 hasAuthorship W2116775017A5088132020 @default.
• W2116775017 hasBestOaLocation W21167750171 @default.
• W2116775017 hasConcept C104317684 @default.
• W2116775017 hasConcept C112190416 @default.
• W2116775017 hasConcept C126322002 @default.
• W2116775017 hasConcept C136449434 @default.
• W2116775017 hasConcept C1621761 @default.
• W2116775017 hasConcept C170493617 @default.
• W2116775017 hasConcept C203014093 @default.
• W2116775017 hasConcept C2776070231 @default.
• W2116775017 hasConcept C2778376644 @default.
• W2116775017 hasConcept C2778384902 @default.
• W2116775017 hasConcept C2779234561 @default.
• W2116775017 hasConcept C2780221984 @default.
• W2116775017 hasConcept C42407357 @default.
• W2116775017 hasConcept C54355233 @default.
• W2116775017 hasConcept C67705224 @default.
• W2116775017 hasConcept C71924100 @default.
• W2116775017 hasConcept C86803240 @default.
• W2116775017 hasConceptScore W2116775017C104317684 @default.
• W2116775017 hasConceptScore W2116775017C112190416 @default.
• W2116775017 hasConceptScore W2116775017C126322002 @default.
• W2116775017 hasConceptScore W2116775017C136449434 @default.
• W2116775017 hasConceptScore W2116775017C1621761 @default.
• W2116775017 hasConceptScore W2116775017C170493617 @default.
• W2116775017 hasConceptScore W2116775017C203014093 @default.
• W2116775017 hasConceptScore W2116775017C2776070231 @default.
• W2116775017 hasConceptScore W2116775017C2778376644 @default.
• W2116775017 hasConceptScore W2116775017C2778384902 @default.
• W2116775017 hasConceptScore W2116775017C2779234561 @default.
• W2116775017 hasConceptScore W2116775017C2780221984 @default.
• W2116775017 hasConceptScore W2116775017C42407357 @default.
• W2116775017 hasConceptScore W2116775017C54355233 @default.
• W2116775017 hasConceptScore W2116775017C67705224 @default.
• W2116775017 hasConceptScore W2116775017C71924100 @default.
• W2116775017 hasConceptScore W2116775017C86803240 @default.
• W2116775017 hasIssue "10" @default.
• W2116775017 hasLocation W21167750171 @default.
• W2116775017 hasLocation W21167750172 @default.
• W2116775017 hasOpenAccess W2116775017 @default.
• W2116775017 hasPrimaryLocation W21167750171 @default.
• W2116775017 hasRelatedWork W1914689199 @default.
• W2116775017 hasRelatedWork W1974055405 @default.
• W2116775017 hasRelatedWork W2048267953 @default.
• W2116775017 hasRelatedWork W2143993175 @default.
• W2116775017 hasRelatedWork W2155279361 @default.
• W2116775017 hasRelatedWork W2793159357 @default.
• W2116775017 hasRelatedWork W3040785646 @default.
• W2116775017 hasRelatedWork W3195069846 @default.
• W2116775017 hasRelatedWork W4224245307 @default.
• W2116775017 hasRelatedWork W2316875459 @default.
• W2116775017 hasVolume "23" @default.
• W2116775017 isParatext "false" @default.
• W2116775017 isRetracted "false" @default.
• W2116775017 magId "2116775017" @default.
• W2116775017 workType "article" @default.