Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116875654> ?p ?o ?g. }
- W2116875654 endingPage "415" @default.
- W2116875654 startingPage "404" @default.
- W2116875654 abstract "Cancer patients with chemotherapy-induced immunosuppression have poor surgical site wound healing. Prior literature supports the use of human adipose-derived stem cell (hASC) lipoinjection to improve wound healing. It has been established that multipotent hASCs facilitate neovascularization, accelerate epithelialization, and quicken wound closure in animal models. Although hASC wound therapy may benefit surgical cancer patients, the chemotherapeutic effects on hASCs are unknown. We hypothesized that paclitaxel, a chemotherapeutic agent, impairs hASC growth, multipotency, and induces apoptosis.hASCs were isolated and harvested from consented, chemotherapy and radiation naive patients. Growth curves, MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide), and EdU (5-ethynyl-2-deoxyguridine) assays measured cytotoxicity and proliferation. Oil Red O stain, Alizarin Red stain, matrigel tube formation assay, and quantitative polymerase chain reaction analyzed hASC differentiation. Annexin V assay measured apoptosis. Immunostaining and Western blot determined tumor necrosis factor α (TNF-α) expression.hASCs were selectively more sensitive to paclitaxel (0.01-30 μM) than fibroblasts (P < 0.05). After 12 d, paclitaxel caused hASC growth arrest, whereas control hASCs proliferated (P = 0.006). Paclitaxel caused an 80.6% reduction in new DNA synthesis (P < 0.001). Paclitaxel severely inhibited endothelial differentiation and capillary-like tube formation. Differentiation markers, lipoprotein lipase (adipogenic), alkaline phosphatase (osteogenic), CD31, and van Willebrand factor (endothelial), were significantly decreased (all P < 0.05) confirming paclitaxel impaired differentiation. Paclitaxel was also found to induce apoptosis and TNF-α was upregulated in paclitaxel-treated hASCs (P < 0.001).Paclitaxel is more cytotoxic to hASCs than fibroblasts. Paclitaxel inhibits hASC proliferation, differentiation, and induces apoptosis, possibly through the TNF-α pathway. Paclitaxel's severe inhibition of endothelial differentiation indicates neovascularization disruption, possibly causing poor wound healing in cancer patients receiving chemotherapy." @default.
- W2116875654 created "2016-06-24" @default.
- W2116875654 creator A5009734430 @default.
- W2116875654 creator A5013295853 @default.
- W2116875654 creator A5037479569 @default.
- W2116875654 creator A5046214153 @default.
- W2116875654 creator A5049109639 @default.
- W2116875654 creator A5052710868 @default.
- W2116875654 creator A5055151897 @default.
- W2116875654 creator A5056933276 @default.
- W2116875654 date "2015-06-01" @default.
- W2116875654 modified "2023-09-29" @default.
- W2116875654 title "Paclitaxel impairs adipose stem cell proliferation and differentiation" @default.
- W2116875654 cites W1567318412 @default.
- W2116875654 cites W169635701 @default.
- W2116875654 cites W1797447800 @default.
- W2116875654 cites W1875639257 @default.
- W2116875654 cites W1888433797 @default.
- W2116875654 cites W1974891161 @default.
- W2116875654 cites W1979005695 @default.
- W2116875654 cites W1985822064 @default.
- W2116875654 cites W1996466160 @default.
- W2116875654 cites W2017251212 @default.
- W2116875654 cites W2021583973 @default.
- W2116875654 cites W2023628461 @default.
- W2116875654 cites W2024013355 @default.
- W2116875654 cites W2024436226 @default.
- W2116875654 cites W2026743018 @default.
- W2116875654 cites W2028412998 @default.
- W2116875654 cites W2031931894 @default.
- W2116875654 cites W2032327857 @default.
- W2116875654 cites W2033484101 @default.
- W2116875654 cites W2033641981 @default.
- W2116875654 cites W2035176325 @default.
- W2116875654 cites W2036348189 @default.
- W2116875654 cites W2038467210 @default.
- W2116875654 cites W2042087846 @default.
- W2116875654 cites W2087905124 @default.
- W2116875654 cites W2096874334 @default.
- W2116875654 cites W2102630679 @default.
- W2116875654 cites W2105931914 @default.
- W2116875654 cites W2107503723 @default.
- W2116875654 cites W2118544754 @default.
- W2116875654 cites W2122199930 @default.
- W2116875654 cites W2127714324 @default.
- W2116875654 cites W2128848742 @default.
- W2116875654 cites W2131489845 @default.
- W2116875654 cites W2135459504 @default.
- W2116875654 cites W2135738344 @default.
- W2116875654 cites W2136112002 @default.
- W2116875654 cites W2138831748 @default.
- W2116875654 cites W2149685956 @default.
- W2116875654 cites W2153405392 @default.
- W2116875654 cites W2158615473 @default.
- W2116875654 cites W2159230992 @default.
- W2116875654 cites W2163910197 @default.
- W2116875654 cites W2170938576 @default.
- W2116875654 cites W2320308628 @default.
- W2116875654 cites W2395763616 @default.
- W2116875654 cites W4255152173 @default.
- W2116875654 cites W4255762755 @default.
- W2116875654 doi "https://doi.org/10.1016/j.jss.2015.03.026" @default.
- W2116875654 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4442730" @default.
- W2116875654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25891676" @default.
- W2116875654 hasPublicationYear "2015" @default.
- W2116875654 type Work @default.
- W2116875654 sameAs 2116875654 @default.
- W2116875654 citedByCount "27" @default.
- W2116875654 countsByYear W21168756542015 @default.
- W2116875654 countsByYear W21168756542016 @default.
- W2116875654 countsByYear W21168756542017 @default.
- W2116875654 countsByYear W21168756542018 @default.
- W2116875654 countsByYear W21168756542019 @default.
- W2116875654 countsByYear W21168756542020 @default.
- W2116875654 countsByYear W21168756542021 @default.
- W2116875654 countsByYear W21168756542022 @default.
- W2116875654 countsByYear W21168756542023 @default.
- W2116875654 crossrefType "journal-article" @default.
- W2116875654 hasAuthorship W2116875654A5009734430 @default.
- W2116875654 hasAuthorship W2116875654A5013295853 @default.
- W2116875654 hasAuthorship W2116875654A5037479569 @default.
- W2116875654 hasAuthorship W2116875654A5046214153 @default.
- W2116875654 hasAuthorship W2116875654A5049109639 @default.
- W2116875654 hasAuthorship W2116875654A5052710868 @default.
- W2116875654 hasAuthorship W2116875654A5055151897 @default.
- W2116875654 hasAuthorship W2116875654A5056933276 @default.
- W2116875654 hasBestOaLocation W21168756541 @default.
- W2116875654 hasConcept C126322002 @default.
- W2116875654 hasConcept C185592680 @default.
- W2116875654 hasConcept C203014093 @default.
- W2116875654 hasConcept C2776694085 @default.
- W2116875654 hasConcept C2777292972 @default.
- W2116875654 hasConcept C2780269544 @default.
- W2116875654 hasConcept C28328180 @default.
- W2116875654 hasConcept C502942594 @default.
- W2116875654 hasConcept C71924100 @default.
- W2116875654 hasConcept C86803240 @default.
- W2116875654 hasConcept C95444343 @default.