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- W2116893732 abstract "Fertilization and ionophore activation of the sea urchin Arbacia punctulata were inhibited in the presence of six analogs of the dye fluorescein. The concentration of any one dye needed for blockage of sperm or Ca-ionophoremediated activation in 50% of the eggs (I50) was a function of the dye's lipid solubility. Substantially higher concentrations of each dye were required to block activation by Ca-ionophore (A23187) than were needed to inhibit sperm activation. A detailed study of the action of Erythrosin B (tetraiodofluorescein) showed that its effects were readily reversible. The I50 concentration of Erythrosin B increased as temperature increased from 10 to 25°C. The kinetics of blockage indicated that Erythrosin B blocked some early step in the program of fertilization. The results suggest that these anionic dyes may inhibit fertilization by preventing successful sperm-egg fusion." @default.
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- W2116893732 date "1981-01-01" @default.
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- W2116893732 title "Fertilization in the sea urchin arbacia punctulata inhibited by fluorescein dyes: Evidence for a plasma membrane mechanism" @default.
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- W2116893732 doi "https://doi.org/10.1002/mrd.1120040306" @default.
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