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- W2116945214 abstract "The alternative sigma factor σH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is dependent upon sigH. To clarify the function of S. coelicolor UshX, both the σH and UshX proteins were overproduced in Escherichia coli and purified. In an in vitro transcription assay, σH, after complementation with S. coelicolor core RNA polymerase, was able to recognize the sigH-dependent promoter, sigH-P2. This transcription was inhibited by UshX, if it was incubated with σH prior to the addition of the core RNA polymerase. When σH and UshX were incubated and electrophoresed through non-denaturing polyacrylamide gels, they formed a specific complex. These results showed that UshX is a specific anti-sigma factor for σH, and the S. coelicolor sigH operon is directly autoregulated." @default.
- W2116945214 created "2016-06-24" @default.
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- W2116945214 date "2002-04-01" @default.
- W2116945214 modified "2023-10-14" @default.
- W2116945214 title "Activity of the<i>Streptomyces coelicolor</i>stress-response sigma factor Ï<sup>H</sup>is regulated by an anti-sigma factor" @default.
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- W2116945214 doi "https://doi.org/10.1111/j.1574-6968.2002.tb11136.x" @default.
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