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• W2116945992 abstract "BACKGROUND: Germinal vesicle transplantation (GVT) provides a means of investigating interactions between karyoplasts and cytoplasts isolated from different cells. Technically, GVT can be accomplished with a high degree of efficiency without compromising the maturation of either the human or mouse oocyte nucleus. Although maturation, fertilization and preimplantation development have been established using GVT, full‐term development has been reported only after supplementation with fresh mature ooplasm. In this study, we assess the ability of immature oocytes collected from gonadotrophin‐primed ovaries to mature in vitro after GVT and develop to full‐term. METHODS: GV oocytes were retrieved from either non‐stimulated or pregnant mare’s serum gonadotrophin (PMSG)‐primed female mice. Microsurgically isolated GV karyoplasts were transplanted into previously enucleated oocytes. Oocytes successfully reconstituted by electrofusion were cultured for 14 h to allow nuclear maturation. Metaphase II oocytes were subjected to Piezo‐ICSI, and those fertilized normally were cultured to the blastocyst stage. Some such embryos were transferred to pseudopregnant female mice to examine their potential for normal development. Cumulus‐denuded non‐manipulated oocytes that were matured in vitro served as controls. RESULTS: The reconstitution and maturation rates were comparable in oocytes isolated from PMSG‐primed and from unstimulated ovaries. The rate of normal fertilization in oocytes from primed ovaries was significantly higher than that of their non‐primed counterparts (63.5 versus 39.6%; P < 0.01). This difference was also confirmed in terms of blastocyst development (31.8 versus 7.9%; P < 0.01). Of a total of 70 embryos transferred to the oviduct of five recipient mice, 21.4% developed to normal live offspring. All developed as normal adults and proved to be fertile. The live birth rate was comparable to that obtained using non‐manipulated control oocytes (22.3%). CONCLUSIONS: Higher rates of fertilization and blastocyst formation were obtained after GVT of mouse oocytes isolated from PMSG‐primed ovaries compared with their non‐primed counterparts. These represent the first mouse offspring derived from in vitro matured, cumulus‐denuded oocytes treated by allo‐GVT and fertilized by ICSI. Thus, GVT appears not to impair oocyte maturation, fertilization and pre‐ and post‐implantation development and, after gonadotrophin priming, allows generation of healthy mouse offspring without mature ooplasm supplementation." @default.
• W2116945992 created "2016-06-24" @default.
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• W2116945992 date "2004-02-12" @default.
• W2116945992 modified "2023-09-27" @default.
• W2116945992 title "A successful model to assess embryo development after transplantation of prophase nuclei" @default.
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• W2116945992 doi "https://doi.org/10.1093/humrep/deh149" @default.
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