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- W2116952685 abstract "A protocol for increasing transcription from plasmid expression vectors is presented. A vector containing chloramphenicol acetyltransferase (CAT) gene was digested leaving the transcription cassette intact. Heat inactivation of restriction enzymes followed by ligation of the digestion products yielded concatemers which migrated as a single band in agarose gel electrophoresis. Mouse fibroblasts transfected with the concatemers gave a CAT activity that was 14-fold greater than that of cells transfected with a similar mass (equimolar gene number) of the native plasmid. The effect was independent of promoter type, restriction enzyme, number of restriction sites and with a noted exception, cell line." @default.
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- W2116952685 date "1997-01-15" @default.
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- W2116952685 title "Transcription from plasmid expression vectors is increased up to 14- fold when plasmids are transfected as concatemers" @default.
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- W2116952685 doi "https://doi.org/10.1093/nar/25.2.449" @default.
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