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• W2116983313 abstract "AbstractIn a genomewide anoikis suppression screen for metastasis genes, we previously identified the neurotrophic receptor tyrosine kinase TrkB. In mouse xenografts, activated TrkB caused highly invasive and metastatic tumors. Here, we describe that TrkB also induces a strong morphological transformation, resembling epithelial-mesenchymal transition (EMT). This required TrkB kinase activity, a functional mitogen-activated protein kinase pathway, suppression of E-cadherin, and induction of Twist, a transcription factor contributing to EMT and metastasis. RNA interference (RNAi)-mediated Twist depletion blocked TrkB-induced EMT-like transformation, anoikis suppression, and growth of tumor xenografts. By searching for essential effectors of TrkB-Twist signaling, we found that Twist induces Snail, another EMT regulator associated with poor cancer prognosis. Snail depletion impaired EMT-like transformation and anoikis suppression induced by TrkB, but in contrast to Twist depletion, it failed to inhibit tumor growth. Instead, Snail RNAi specifically impaired the formation of lung metastases. Epistasis experiments suggested that Twist acts upstream from Snail. Our results demonstrate that TrkB signaling activates a Twist-Snail axis that is critically involved in EMT-like transformation, tumorigenesis, and metastasis. Moreover, our data shed more light on the epistatic relationship between Twist and Snail, two key transcriptional regulators of EMT and metastasis. SUPPLEMENTAL MATERIALSupplemental material for this article may be found at http://mcb.asm.org/ .ACKNOWLEDGMENTSWe thank M. Voetel, S. Greven, H. Grimminck, and all animal caretakers for their excellent technical help with the in vivo experiments; L. Brocks and L. Oomen for advice with image analysis; and the experimental animal pathology department and J. Zevenhoven for help with the pathological analysis. We thank M. Heimerikx, M. Nieuwland, J. de Ronde, D. Sie, and R. Kerkhoven for performing microarray analysis; C. Desmet for communication of results prior to publication; N. Armstrong for statistical advice; A. Pfauth and F. van Diepen for help with fluorescence-activated cell sorting; and S. Ellenbroek and S. Mertens for advice on RAC activity assays. We thank all members of the Peeper laboratory for their valuable input and A. Prieur and C. Hömig-Hölzel for critical reading of the manuscript. We thank K. Becker for the Snail antibody, C. Niessen for the E-cadherin plasmid, A. Munoz for the Snail-HA plasmid, and R. Weinberg for the Twist plasmid.This work was supported by the Dutch Cancer Society (KWF) grant to M.A.S. and D.S.P. and a European Union FP6 grant to T.R.G. and D.S.P. D.S.P. is also supported by the European Molecular Biology Organization (EMBO) Young Investigator program and by a KWF Queen Wilhelmina program grant." @default.
• W2116983313 created "2016-06-24" @default.
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• W2116983313 date "2009-07-01" @default.
• W2116983313 modified "2023-09-30" @default.
• W2116983313 title "A Twist-Snail Axis Critical for TrkB-Induced Epithelial-Mesenchymal Transition-Like Transformation, Anoikis Resistance, and Metastasis" @default.
• W2116983313 cites W1484020898 @default.
• W2116983313 cites W1964118513 @default.
• W2116983313 cites W1964595762 @default.
• W2116983313 cites W1965255998 @default.
• W2116983313 cites W1968783143 @default.
• W2116983313 cites W1973523055 @default.
• W2116983313 cites W1974643611 @default.
• W2116983313 cites W1987473368 @default.
• W2116983313 cites W1987641860 @default.
• W2116983313 cites W1988218110 @default.
• W2116983313 cites W1988824186 @default.
• W2116983313 cites W1989963027 @default.
• W2116983313 cites W1994242450 @default.
• W2116983313 cites W1995378553 @default.
• W2116983313 cites W2007754809 @default.
• W2116983313 cites W2010685617 @default.
• W2116983313 cites W2014691022 @default.
• W2116983313 cites W2017331095 @default.
• W2116983313 cites W2022346609 @default.
• W2116983313 cites W2023046316 @default.
• W2116983313 cites W2025647419 @default.
• W2116983313 cites W2030751364 @default.
• W2116983313 cites W2033148943 @default.
• W2116983313 cites W2036008499 @default.
• W2116983313 cites W2036832250 @default.
• W2116983313 cites W2042905219 @default.
• W2116983313 cites W2042965279 @default.
• W2116983313 cites W2048136762 @default.
• W2116983313 cites W2048735580 @default.
• W2116983313 cites W2050867644 @default.
• W2116983313 cites W2053195947 @default.
• W2116983313 cites W2057303779 @default.
• W2116983313 cites W2063011414 @default.
• W2116983313 cites W2065903778 @default.
• W2116983313 cites W2067606868 @default.
• W2116983313 cites W2069346669 @default.
• W2116983313 cites W2073079990 @default.
• W2116983313 cites W2074747200 @default.
• W2116983313 cites W2076583198 @default.
• W2116983313 cites W2076894554 @default.
• W2116983313 cites W2081975691 @default.
• W2116983313 cites W2084769511 @default.
• W2116983313 cites W2086262311 @default.
• W2116983313 cites W2087367170 @default.
• W2116983313 cites W2089693511 @default.
• W2116983313 cites W2091864211 @default.
• W2116983313 cites W2093226717 @default.
• W2116983313 cites W2093921289 @default.
• W2116983313 cites W2097829282 @default.
• W2116983313 cites W2109467838 @default.
• W2116983313 cites W2113328155 @default.
• W2116983313 cites W2115095574 @default.
• W2116983313 cites W2121665844 @default.
• W2116983313 cites W2124559417 @default.
• W2116983313 cites W2124580184 @default.
• W2116983313 cites W2132740988 @default.
• W2116983313 cites W2136280424 @default.
• W2116983313 cites W2137022580 @default.
• W2116983313 cites W2140551998 @default.
• W2116983313 cites W2142746886 @default.
• W2116983313 cites W2146445411 @default.
• W2116983313 cites W2146678830 @default.
• W2116983313 cites W2147865927 @default.
• W2116983313 cites W2147960762 @default.
• W2116983313 cites W2154727263 @default.
• W2116983313 cites W2157001082 @default.
• W2116983313 cites W2161025693 @default.
• W2116983313 cites W2162075740 @default.
• W2116983313 cites W2163740123 @default.
• W2116983313 cites W2165901622 @default.
• W2116983313 cites W2171109371 @default.
• W2116983313 cites W2189490860 @default.
• W2116983313 cites W4297944786 @default.
• W2116983313 doi "https://doi.org/10.1128/mcb.01164-08" @default.
• W2116983313 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2698746" @default.
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• W2116983313 hasPublicationYear "2009" @default.
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