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- W2116990909 abstract "We characterized the effect of femtosecond pulse trains on gastrulating Drosophila embryos using two-photon-excited fluorescence (2PEF) and third-harmonic generation (THG) microscopy. Femtosecond pulses can be used to perform controlled intravital microdissections that alter the embryo structural integrity but do not significantly perturb cytoskeleton dynamics in adjacent cells. Such targeted ablations can be used to remotely perturb cell movements in developing embryos. The transparent combination of femtosecond pulse-induced ablation with nonlinear microscopy can be used to analyze in vivo the effect of cell and tissue deformations. Der Effekt von Femtosekunden-Pulsen auf die Gastrulation von Drosophila Embryonen wurde mit Hilfe von Zwei-Photonen Fluoreszenz- und THG-Mikroskopie charakterisiert. Femtosekunden-Pulse können dazu verwendet werden, kontrollierte, intravitale Mikrodissektionen durchzuführen, die die strukturelle Integrität des Embryos verändern, aber die Cytoskelettdynamik in den umgebenden Zellen nicht wesentlich beeinflussen. Mit Hilfe dieser gezielten Ablationen können Zellbewegungen in sich entwickelnden Embryonen von fern beeinflusst werden. Die direkte Verbindung von Femtosekunden-Puls-induzierter Ablation mit nichtlinearer Mikroskopie kann dazu verwendet werden, in vivo den Effekt von Formveränderungen von Zellen und Geweben zu untersuchen." @default.
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- W2116990909 date "2005-10-01" @default.
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- W2116990909 title "Femtosecond pulse-induced microprocessing of live Drosophila embryos" @default.
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- W2116990909 doi "https://doi.org/10.1016/j.mla.2005.07.001" @default.
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