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- W2117021095 abstract "Natural products (secondary metabolites) are a rich source of compounds with important biological activities. Eliciting pathway expression is always challenging but extremely important in natural product discovery because an individual pathway is tightly controlled through a unique regulation mechanism and hence often remains silent under the routine culturing conditions. To overcome the drawbacks of the traditional approaches that lack general applicability, we developed a simple synthetic biology approach that decouples pathway expression from complex native regulations. Briefly, the entire silent biosynthetic pathway is refactored using a plug-and-play scaffold and a set of heterologous promoters that are functional in a heterologous host under the target culturing condition. Using this strategy, we successfully awakened the silent spectinabilin pathway from Streptomyces orinoci. This strategy bypasses the traditional laborious processes to elicit pathway expression and represents a new platform for discovering novel natural products." @default.
- W2117021095 created "2016-06-24" @default.
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- W2117021095 date "2013-08-22" @default.
- W2117021095 modified "2023-10-16" @default.
- W2117021095 title "Refactoring the Silent Spectinabilin Gene Cluster Using a Plug-and-Play Scaffold" @default.
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- W2117021095 doi "https://doi.org/10.1021/sb400058n" @default.
- W2117021095 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4326240" @default.
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