FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117047559> ?p ?o ?g. }

• W2117047559 endingPage "81" @default.
• W2117047559 startingPage "73" @default.
• W2117047559 abstract "ABSTRACT We describe a general method for analyzing the genetic fine structure of plasmid-borne genes in yeast. Previously we had reported that a linearized plasmid is efficiently rescued by recombination with a homologous restriction fragment when these are co-introduced by DNA-mediated transformation of yeast. Here, we show that a mutation can be localized to a small DNA interval when members of a deletion series of wild-type restriction fragments are used in the rescue of a linearized mutant plasmid. The resolution of this method is to at least 30 base pairs and is limited by the loss of a wild-type marker with proximity to a free DNA end. As a means for establishing the nonidentity of two mutations, we determined the resolution of two-point crosses with a mutant linearized plasmid and a mutant homologous restriction fragment. Recombination between mutations separated by as little as 100 base pairs was detected. Moreover, the results indicate that exchange within a marked interval results primarily from one of two single crossovers that repair the linearized plasmid. These approaches to mapping the genetic fine structure of plasmids should join existing methods in a robust approach to the mutational analysis of gene structure in yeast." @default.
• W2117047559 created "2016-06-24" @default.
• W2117047559 creator A5034321470 @default.
• W2117047559 creator A5036550616 @default.
• W2117047559 creator A5076814321 @default.
• W2117047559 creator A5077210953 @default.
• W2117047559 creator A5077542898 @default.
• W2117047559 date "1987-01-01" @default.
• W2117047559 modified "2023-10-01" @default.
• W2117047559 title "Fine Structure Recombinational Analysis of Cloned Genes Using Yeast Transformation" @default.
• W2117047559 cites W1433017100 @default.
• W2117047559 cites W1526043578 @default.
• W2117047559 cites W1546783991 @default.
• W2117047559 cites W156338606 @default.
• W2117047559 cites W1971724293 @default.
• W2117047559 cites W1981741490 @default.
• W2117047559 cites W1985910275 @default.
• W2117047559 cites W2006945555 @default.
• W2117047559 cites W2010911270 @default.
• W2117047559 cites W2011972566 @default.
• W2117047559 cites W2022016707 @default.
• W2117047559 cites W2028782768 @default.
• W2117047559 cites W2033662557 @default.
• W2117047559 cites W2034252391 @default.
• W2117047559 cites W2035400738 @default.
• W2117047559 cites W2045251366 @default.
• W2117047559 cites W2048380771 @default.
• W2117047559 cites W2056312081 @default.
• W2117047559 cites W2081207326 @default.
• W2117047559 cites W2087777628 @default.
• W2117047559 cites W2109773803 @default.
• W2117047559 cites W2118533974 @default.
• W2117047559 cites W2141356573 @default.
• W2117047559 cites W2166028173 @default.
• W2117047559 cites W2090920017 @default.
• W2117047559 doi "https://doi.org/10.1093/genetics/115.1.73" @default.
• W2117047559 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1203065" @default.
• W2117047559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3549444" @default.
• W2117047559 hasPublicationYear "1987" @default.
• W2117047559 type Work @default.
• W2117047559 sameAs 2117047559 @default.
• W2117047559 citedByCount "45" @default.
• W2117047559 countsByYear W21170475592012 @default.
• W2117047559 countsByYear W21170475592016 @default.
• W2117047559 countsByYear W21170475592018 @default.
• W2117047559 countsByYear W21170475592021 @default.
• W2117047559 countsByYear W21170475592022 @default.
• W2117047559 crossrefType "journal-article" @default.
• W2117047559 hasAuthorship W2117047559A5034321470 @default.
• W2117047559 hasAuthorship W2117047559A5036550616 @default.
• W2117047559 hasAuthorship W2117047559A5076814321 @default.
• W2117047559 hasAuthorship W2117047559A5077210953 @default.
• W2117047559 hasAuthorship W2117047559A5077542898 @default.
• W2117047559 hasBestOaLocation W21170475592 @default.
• W2117047559 hasConcept C101434241 @default.
• W2117047559 hasConcept C102744134 @default.
• W2117047559 hasConcept C104317684 @default.
• W2117047559 hasConcept C128319531 @default.
• W2117047559 hasConcept C135300049 @default.
• W2117047559 hasConcept C143065580 @default.
• W2117047559 hasConcept C156695909 @default.
• W2117047559 hasConcept C170673058 @default.
• W2117047559 hasConcept C204241405 @default.
• W2117047559 hasConcept C22744801 @default.
• W2117047559 hasConcept C501734568 @default.
• W2117047559 hasConcept C54355233 @default.
• W2117047559 hasConcept C552990157 @default.
• W2117047559 hasConcept C58301748 @default.
• W2117047559 hasConcept C86803240 @default.
• W2117047559 hasConceptScore W2117047559C101434241 @default.
• W2117047559 hasConceptScore W2117047559C102744134 @default.
• W2117047559 hasConceptScore W2117047559C104317684 @default.
• W2117047559 hasConceptScore W2117047559C128319531 @default.
• W2117047559 hasConceptScore W2117047559C135300049 @default.
• W2117047559 hasConceptScore W2117047559C143065580 @default.
• W2117047559 hasConceptScore W2117047559C156695909 @default.
• W2117047559 hasConceptScore W2117047559C170673058 @default.
• W2117047559 hasConceptScore W2117047559C204241405 @default.
• W2117047559 hasConceptScore W2117047559C22744801 @default.
• W2117047559 hasConceptScore W2117047559C501734568 @default.
• W2117047559 hasConceptScore W2117047559C54355233 @default.
• W2117047559 hasConceptScore W2117047559C552990157 @default.
• W2117047559 hasConceptScore W2117047559C58301748 @default.
• W2117047559 hasConceptScore W2117047559C86803240 @default.
• W2117047559 hasIssue "1" @default.
• W2117047559 hasLocation W21170475591 @default.
• W2117047559 hasLocation W21170475592 @default.
• W2117047559 hasOpenAccess W2117047559 @default.
• W2117047559 hasPrimaryLocation W21170475591 @default.
• W2117047559 hasRelatedWork W11158071 @default.
• W2117047559 hasRelatedWork W1988835787 @default.
• W2117047559 hasRelatedWork W2095289451 @default.
• W2117047559 hasRelatedWork W2114246483 @default.
• W2117047559 hasRelatedWork W2117047559 @default.
• W2117047559 hasRelatedWork W2123987714 @default.
• W2117047559 hasRelatedWork W2151517123 @default.
• W2117047559 hasRelatedWork W2438679054 @default.
• W2117047559 hasRelatedWork W4239613700 @default.

• next