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- W2117051152 abstract "The cDNA for human squalene monooxygenase, a key enzyme in the committed pathway for cholesterol biosynthesis, was amplified from a human liver cDNA library and cloned, and the protein was expressed in Escherichia coli and purified. Kinetic analysis of the purified enzyme revealed an apparent Km for squalene of 7.7 μM and an apparent kcat of 1.1 min−1. For FAD the apparent Km is 0.3 μM, consistent with a loosely bound flavin. The apparent Km for NADPH–cytochrome P450 reductase, the requisite electron transfer partner, is 14 nM. The amount of reductase needed for maximal activity is about threefold less than the amount of squalene monooxygenase present in the assay; thus, electron transfer to the monooxygenase is not likely to be rate limiting. Previous reports have implicated inhibition of this enzyme as the cause of a peripheral demyelination seen in weanling rats fed a diet containing tellurium. As no data were available for humans, the ability of a number of tellurium and related elemental compounds to inhibit the recombinant human enzyme was examined. Tellurite, tellurium dioxide, selenite, and selenium dioxide were inhibitory; the tellurium compounds were more potent than the selenium compounds, as indicated by their IC50 values (17 and 37 μM, respectively). Kinetic analysis of the inhibition by tellurite suggests multiple sites of interaction with the enzyme in a noncompetitive manner with respect to squalene." @default.
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- W2117051152 date "2000-02-01" @default.
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- W2117051152 title "Cloning, Heterologous Expression, and Enzymological Characterization of Human Squalene Monooxygenase" @default.
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- W2117051152 doi "https://doi.org/10.1006/abbi.1999.1629" @default.
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