FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117081330> ?p ?o ?g. }

• W2117081330 endingPage "234" @default.
• W2117081330 startingPage "225" @default.
• W2117081330 abstract "Research is progressing fast to find safe and effective methods of delivering therapeutic genes to patients afflicted with a range of genetic and acquired diseases that either do not respond at all, or respond poorly, to treatment with small‐molecule drugs or protein‐replacement therapy. A technical barrier that remains relates to the need for scalable operations that can consistently and reproducibly make large quantities of the therapeutic gene vectors under the current Good Manufacturing Practice (‘cGMP’). The present investigation focuses on these issues and introduces a new method of assessing the engineering effects of process and material factors on the colloidal properties of plasmid‐DNA delivery systems based on response surface methodology (RSM) and experimental techniques. Previously, experiments have shown that several factors can reduce the physical stability of non‐viral delivery systems. Specifically, it has been demonstrated that the mean size and charge of plasmid DNA condensed by cationic agents are affected by many factors, including the pH and ionic strength of the buffer, and the method of preparation. For example, the method and intensity of mixing of the DNA with condensing and conjugating agents have been shown to be important. Using RSM to analyse new experimental data in the present paper, we report on the impact of these factors and, more crucially, the effects of interaction between the factors on the colloidal properties of the DNA–vector complexes. Specifically, for plasmid DNA condensed by poly‐ l ‐lysine, interactions between ionic strength, pH and DNA concentrations play a critical role. Whether poly‐ l ‐lysine should be used as a condensing agent in the final delivery system remains to be demonstrated. However, the use of RSM combined with the scaleable experimental approach described in this paper may be applied to other delivery systems." @default.
• W2117081330 created "2016-06-24" @default.
• W2117081330 creator A5013493955 @default.
• W2117081330 creator A5016993821 @default.
• W2117081330 creator A5023584058 @default.
• W2117081330 creator A5041356395 @default.
• W2117081330 creator A5063785042 @default.
• W2117081330 creator A5069670845 @default.
• W2117081330 date "2003-06-01" @default.
• W2117081330 modified "2023-10-17" @default.
• W2117081330 title "The influence of physico-chemical and process conditions on the physical stability of plasmid DNA complexes using response surface methodology" @default.
• W2117081330 cites W1968902554 @default.
• W2117081330 cites W1973131055 @default.
• W2117081330 cites W1976532926 @default.
• W2117081330 cites W1982086117 @default.
• W2117081330 cites W1986364564 @default.
• W2117081330 cites W1998154525 @default.
• W2117081330 cites W1999313169 @default.
• W2117081330 cites W2006118571 @default.
• W2117081330 cites W2007668855 @default.
• W2117081330 cites W2023848643 @default.
• W2117081330 cites W2025372297 @default.
• W2117081330 cites W2037215798 @default.
• W2117081330 cites W2043764483 @default.
• W2117081330 cites W2054310182 @default.
• W2117081330 cites W2077325967 @default.
• W2117081330 cites W2082204799 @default.
• W2117081330 cites W2090999404 @default.
• W2117081330 cites W2114269429 @default.
• W2117081330 cites W2130433105 @default.
• W2117081330 cites W2131577188 @default.
• W2117081330 cites W2132184590 @default.
• W2117081330 cites W2168206768 @default.
• W2117081330 cites W2324654634 @default.
• W2117081330 cites W3083094549 @default.
• W2117081330 doi "https://doi.org/10.1042/ba20030012" @default.
• W2117081330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12683954" @default.
• W2117081330 hasPublicationYear "2003" @default.
• W2117081330 type Work @default.
• W2117081330 sameAs 2117081330 @default.
• W2117081330 citedByCount "14" @default.
• W2117081330 countsByYear W21170813302012 @default.
• W2117081330 countsByYear W21170813302013 @default.
• W2117081330 countsByYear W21170813302016 @default.
• W2117081330 crossrefType "journal-article" @default.
• W2117081330 hasAuthorship W2117081330A5013493955 @default.
• W2117081330 hasAuthorship W2117081330A5016993821 @default.
• W2117081330 hasAuthorship W2117081330A5023584058 @default.
• W2117081330 hasAuthorship W2117081330A5041356395 @default.
• W2117081330 hasAuthorship W2117081330A5063785042 @default.
• W2117081330 hasAuthorship W2117081330A5069670845 @default.
• W2117081330 hasConcept C104317684 @default.
• W2117081330 hasConcept C111599444 @default.
• W2117081330 hasConcept C111919701 @default.
• W2117081330 hasConcept C12554922 @default.
• W2117081330 hasConcept C135983454 @default.
• W2117081330 hasConcept C145148216 @default.
• W2117081330 hasConcept C150077022 @default.
• W2117081330 hasConcept C171250308 @default.
• W2117081330 hasConcept C178790620 @default.
• W2117081330 hasConcept C184651966 @default.
• W2117081330 hasConcept C185592680 @default.
• W2117081330 hasConcept C186060115 @default.
• W2117081330 hasConcept C187428577 @default.
• W2117081330 hasConcept C192562407 @default.
• W2117081330 hasConcept C2182769 @default.
• W2117081330 hasConcept C22744801 @default.
• W2117081330 hasConcept C41008148 @default.
• W2117081330 hasConcept C43617362 @default.
• W2117081330 hasConcept C552990157 @default.
• W2117081330 hasConcept C55493867 @default.
• W2117081330 hasConcept C59789625 @default.
• W2117081330 hasConcept C70721500 @default.
• W2117081330 hasConcept C86803240 @default.
• W2117081330 hasConcept C98045186 @default.
• W2117081330 hasConceptScore W2117081330C104317684 @default.
• W2117081330 hasConceptScore W2117081330C111599444 @default.
• W2117081330 hasConceptScore W2117081330C111919701 @default.
• W2117081330 hasConceptScore W2117081330C12554922 @default.
• W2117081330 hasConceptScore W2117081330C135983454 @default.
• W2117081330 hasConceptScore W2117081330C145148216 @default.
• W2117081330 hasConceptScore W2117081330C150077022 @default.
• W2117081330 hasConceptScore W2117081330C171250308 @default.
• W2117081330 hasConceptScore W2117081330C178790620 @default.
• W2117081330 hasConceptScore W2117081330C184651966 @default.
• W2117081330 hasConceptScore W2117081330C185592680 @default.
• W2117081330 hasConceptScore W2117081330C186060115 @default.
• W2117081330 hasConceptScore W2117081330C187428577 @default.
• W2117081330 hasConceptScore W2117081330C192562407 @default.
• W2117081330 hasConceptScore W2117081330C2182769 @default.
• W2117081330 hasConceptScore W2117081330C22744801 @default.
• W2117081330 hasConceptScore W2117081330C41008148 @default.
• W2117081330 hasConceptScore W2117081330C43617362 @default.
• W2117081330 hasConceptScore W2117081330C552990157 @default.
• W2117081330 hasConceptScore W2117081330C55493867 @default.
• W2117081330 hasConceptScore W2117081330C59789625 @default.
• W2117081330 hasConceptScore W2117081330C70721500 @default.
• W2117081330 hasConceptScore W2117081330C86803240 @default.

• next