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• W2117130601 abstract "Work reported here shows the outcome of improvements made to a published PCR-RFLP approach for fish species identification. The objective of the improved method was to replace the gel-electrophoretic steps for fragment separation, detection and analysis, by employing a chip-based capillary electrophoresis (CE) system. Fragment resolution on the system was sensitive, with detection of small fragments not observed with the published conventional gel-based method. Experimental repeatability was less than 3%, allowing species identification without the need to run reference materials with every sample. Using DNA admixtures, the discrimination of 5% salmon DNA in trout DNA was readily achieved. Results showed that the CE system was quick and easy to use, providing post-restriction digestion results for 12 samples within 40 min. This, along with the relatively low cost of the instrument, should make this method suitable for use in a wide range of analytical laboratories involved with species identification." @default.
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• W2117130601 date "2005-09-01" @default.
• W2117130601 modified "2023-09-23" @default.
• W2117130601 title "Improved fish species identification by use of lab-on-a-chip technology" @default.
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• W2117130601 doi "https://doi.org/10.1016/j.foodcont.2004.06.022" @default.
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