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- W2117227929 abstract "Saffron (Crocus sativus) is the most valuable and indigenous crop in Iran. The stigmas of flower are used asa popular natural flavouring, colouring and medicinal agent. However, the market suffers from frauds in thisplant such as mixing with safflower petals due to high profit. Identification of these frauds with conventionaland biochemical methods is difficult and low sensitive. Therefore, application of molecular markers such asrandom amplified polymorphic DNA (RAPD)/sequence characterized amplified regions (SCAR) is beingconsidered as an alternative. In this study, DNA was extracted from dry stigmas of 5 Saffron accessions anddry petals of 7 safflower cultivars. RAPD reactions with ten 15-mer random primers resulted in two specificmonomorphic bands (500 and 700 bp) for safflower, while they were absent in saffron accessions. PCRanalysis with specific SCAR primers amplified two specific bands (414 and 589 bp) for safflowers in differentcombinations of saffron stigmas and safflower petals. This was the case with very low rates or 1% ofsafflower. Therefore, this method seems to be suitable for fraud identification of safflower petals incommercial saffron samples." @default.
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- W2117227929 date "2011-06-01" @default.
- W2117227929 modified "2023-09-23" @default.
- W2117227929 title "Identification of Safflower as a fraud in commercial Saffron using RAPD/SCAR" @default.
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- W2117227929 doi "https://doi.org/10.22067/jcmr.v3i1.13116" @default.
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