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- W2117255121 abstract "Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and the transcriptional activator XYR1 and the carbon catabolite repressor CRE1 are main regulators of their expression. We quantified the nucleo-cytoplasmic shuttling dynamics of GFP-fusion proteins of both transcription factors under cellulase and xylanase inducing conditions, and correlated their nuclear presence/absence with transcriptional changes. We also compared their subcellular localization in conidial germlings and mature hyphae. We show that cellulase gene expression requires de novo biosynthesis of XYR1 and its simultaneous nuclear import, whereas carbon catabolite repression is regulated through preformed CRE1 imported from the cytoplasmic pool. Termination of induction immediately stopped cellulase gene transcription and was accompanied by rapid nuclear degradation of XYR1. In contrast, nuclear CRE1 rapidly decreased upon glucose depletion, and became recycled into the cytoplasm. In mature hyphae, nuclei containing activated XYR1 were concentrated in the colony center, indicating that this is the main region of XYR1 synthesis and cellulase transcription. CRE1 was found to be evenly distributed throughout the entire mycelium. Taken together, our data revealed novel aspects of the dynamic shuttling and spatial bias of the major regulator of (hemi-)cellulase gene expression, XYR1, in T. reesei." @default.
- W2117255121 created "2016-06-24" @default.
- W2117255121 creator A5002401002 @default.
- W2117255121 creator A5063438806 @default.
- W2117255121 creator A5070750713 @default.
- W2117255121 creator A5073288256 @default.
- W2117255121 date "2014-10-29" @default.
- W2117255121 modified "2023-10-16" @default.
- W2117255121 title "Nucleo‐cytoplasmic shuttling dynamics of the transcriptional regulators<scp>XYR1</scp>and<scp>CRE1</scp>under conditions of cellulase and xylanase gene expression in<scp><i>T</i></scp><i>richoderma reesei</i>" @default.
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- W2117255121 doi "https://doi.org/10.1111/mmi.12824" @default.
- W2117255121 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4282317" @default.
- W2117255121 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25302561" @default.
- W2117255121 hasPublicationYear "2014" @default.
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