Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117325612> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2117325612 abstract "One potential noninvasive approach for antenatal diagnosis is the use of fetal cells in maternal circulation as a source of fetal DNA (1). To date, fetal DNA has been extracted from maternal nucleated blood cells and amplified by nested PCR of Y-chromosome sequences from male fetuses (2)(3).A recent study (4) demonstrated the presence of fetal DNA in maternal plasma. After a single PCR with a high number of cycles, detection of fetal DNA was possible in 24 (80%) of the 30 maternal plasma samples, using 10 μL of boiled plasma (4). When DNA extracted from a similar volume of whole blood was used, only 17% of the samples gave a Y-positive signal, suggesting an enrichment of fetal DNA in plasma compared with whole blood cells (4). The mechanism responsible for fetal DNA release in maternal circulation is unclear. It has been postulated that fetal cell lysis after physical and immunological damage, as well as developmentally regulated apoptosis of certain fetal tissues, may be responsible for this phenomenon (5). Furthermore, the release and clearance of fetal DNA from maternal plasma may be influenced by pathophysiological factors that are still to be determined.The efficacy of the single PCR protocol on maternal plasma DNA has not yet been compared to established methodologies of nested amplification of DNA prepared from intact cells. The present work compares single PCR on plasma DNA with nested PCR on DNA extracted from plasma or nucleated blood cells. We collected 27 maternal blood samples at gestational ages ranging from the 7th to the 32nd weeks (16 male- and 11 female-bearing pregnancies). Nine, 14, and 4 samples were from the first (7–12 weeks), second (14–20 weeks), and third (27–32 weeks) trimesters. Fetal gender was unknown at the time of sampling and analysis and was verified …" @default.
- W2117325612 created "2016-06-24" @default.
- W2117325612 creator A5011650530 @default.
- W2117325612 creator A5020208332 @default.
- W2117325612 creator A5034302518 @default.
- W2117325612 creator A5080993076 @default.
- W2117325612 creator A5081790996 @default.
- W2117325612 creator A5089876387 @default.
- W2117325612 date "1999-09-01" @default.
- W2117325612 modified "2023-09-26" @default.
- W2117325612 title "Evaluation of Different Approaches for Fetal DNA Analysis from Maternal Plasma and Nucleated Blood Cells" @default.
- W2117325612 cites W2034083499 @default.
- W2117325612 cites W2087062217 @default.
- W2117325612 cites W2100977485 @default.
- W2117325612 cites W2115865929 @default.
- W2117325612 cites W2171525147 @default.
- W2117325612 doi "https://doi.org/10.1093/clinchem/45.9.1570" @default.
- W2117325612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10471665" @default.
- W2117325612 hasPublicationYear "1999" @default.
- W2117325612 type Work @default.
- W2117325612 sameAs 2117325612 @default.
- W2117325612 citedByCount "33" @default.
- W2117325612 countsByYear W21173256122012 @default.
- W2117325612 countsByYear W21173256122013 @default.
- W2117325612 countsByYear W21173256122014 @default.
- W2117325612 countsByYear W21173256122015 @default.
- W2117325612 countsByYear W21173256122016 @default.
- W2117325612 countsByYear W21173256122017 @default.
- W2117325612 crossrefType "journal-article" @default.
- W2117325612 hasAuthorship W2117325612A5011650530 @default.
- W2117325612 hasAuthorship W2117325612A5020208332 @default.
- W2117325612 hasAuthorship W2117325612A5034302518 @default.
- W2117325612 hasAuthorship W2117325612A5080993076 @default.
- W2117325612 hasAuthorship W2117325612A5081790996 @default.
- W2117325612 hasAuthorship W2117325612A5089876387 @default.
- W2117325612 hasBestOaLocation W21173256121 @default.
- W2117325612 hasConcept C104317684 @default.
- W2117325612 hasConcept C134018914 @default.
- W2117325612 hasConcept C152110520 @default.
- W2117325612 hasConcept C153911025 @default.
- W2117325612 hasConcept C16685009 @default.
- W2117325612 hasConcept C172680121 @default.
- W2117325612 hasConcept C2776575606 @default.
- W2117325612 hasConcept C2778258057 @default.
- W2117325612 hasConcept C2779234561 @default.
- W2117325612 hasConcept C2780246931 @default.
- W2117325612 hasConcept C49105822 @default.
- W2117325612 hasConcept C54355233 @default.
- W2117325612 hasConcept C552990157 @default.
- W2117325612 hasConcept C71924100 @default.
- W2117325612 hasConcept C86803240 @default.
- W2117325612 hasConceptScore W2117325612C104317684 @default.
- W2117325612 hasConceptScore W2117325612C134018914 @default.
- W2117325612 hasConceptScore W2117325612C152110520 @default.
- W2117325612 hasConceptScore W2117325612C153911025 @default.
- W2117325612 hasConceptScore W2117325612C16685009 @default.
- W2117325612 hasConceptScore W2117325612C172680121 @default.
- W2117325612 hasConceptScore W2117325612C2776575606 @default.
- W2117325612 hasConceptScore W2117325612C2778258057 @default.
- W2117325612 hasConceptScore W2117325612C2779234561 @default.
- W2117325612 hasConceptScore W2117325612C2780246931 @default.
- W2117325612 hasConceptScore W2117325612C49105822 @default.
- W2117325612 hasConceptScore W2117325612C54355233 @default.
- W2117325612 hasConceptScore W2117325612C552990157 @default.
- W2117325612 hasConceptScore W2117325612C71924100 @default.
- W2117325612 hasConceptScore W2117325612C86803240 @default.
- W2117325612 hasLocation W21173256121 @default.
- W2117325612 hasLocation W21173256122 @default.
- W2117325612 hasOpenAccess W2117325612 @default.
- W2117325612 hasPrimaryLocation W21173256121 @default.
- W2117325612 hasRelatedWork W1920471819 @default.
- W2117325612 hasRelatedWork W1966646203 @default.
- W2117325612 hasRelatedWork W1980837114 @default.
- W2117325612 hasRelatedWork W1988200035 @default.
- W2117325612 hasRelatedWork W2003130419 @default.
- W2117325612 hasRelatedWork W2040041836 @default.
- W2117325612 hasRelatedWork W2078927009 @default.
- W2117325612 hasRelatedWork W2080501218 @default.
- W2117325612 hasRelatedWork W2083027033 @default.
- W2117325612 hasRelatedWork W2092479048 @default.
- W2117325612 hasRelatedWork W2097491292 @default.
- W2117325612 hasRelatedWork W2100977485 @default.
- W2117325612 hasRelatedWork W2108546855 @default.
- W2117325612 hasRelatedWork W2114755369 @default.
- W2117325612 hasRelatedWork W2115865929 @default.
- W2117325612 hasRelatedWork W2132158935 @default.
- W2117325612 hasRelatedWork W2140501113 @default.
- W2117325612 hasRelatedWork W2166688382 @default.
- W2117325612 hasRelatedWork W2314340472 @default.
- W2117325612 hasRelatedWork W2439931709 @default.
- W2117325612 isParatext "false" @default.
- W2117325612 isRetracted "false" @default.
- W2117325612 magId "2117325612" @default.
- W2117325612 workType "article" @default.