FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117442345> ?p ?o ?g. }

• W2117442345 endingPage "8318" @default.
• W2117442345 startingPage "8307" @default.
• W2117442345 abstract "ABSTRACT Monoclonal-antibody (MAb)-resistant mutants were used to map antigenic sites on foot-and-mouth disease virus (FMDV), which resulted in the identification of neutralizing epitopes in the flexible βG-βH loop in VP1. For FMDV SAT2 viruses, studies have shown that at least two antigenic sites exist. By use of an infectious SAT2 cDNA clone, 10 structurally exposed and highly variable loops were identified as putative antigenic sites on the VP1, VP2, and VP3 capsid proteins of SAT2/Zimbabwe (ZIM)/7/83 (topotype II) and replaced with the corresponding regions of SAT2/Kruger National Park (KNP)/19/89 (topotype I). Virus neutralization assays using convalescent-phase antisera raised against the parental virus, SAT2/ZIM/7/83, indicated that the mutant virus containing the TQQS-to-ETPV mutation in the N-terminal part of the βG-βH loop of VP1 showed not only a significant increase in the neutralization titer but also an increase in the index of avidity to the convalescent-phase antisera. Furthermore, antigenic profiling of the epitope-replaced and parental viruses with nonneutralizing SAT2-specific MAbs led to the identification of two nonneutralizing antigenic regions. Both regions were mapped to incorporate residues 71 to 72 of VP2 as the major contact point. The binding footprint of one of the antigenic regions encompasses residues 71 to 72 and 133 to 134 of VP2 and residues 48 to 50 of VP1, and the second antigenic region encompasses residues 71 to 72 and 133 to 134 of VP2 and residues 84 to 86 and 109 to 11 of VP1. This is the first time that antigenic regions encompassing residues 71 to 72 of VP2 have been identified on the capsid of a SAT2 FMDV. IMPORTANCE Monoclonal-antibody-resistant mutants have traditionally been used to map antigenic sites on foot-and-mouth disease virus (FMDV). However, for SAT2-type viruses, which are responsible for most of the FMD outbreaks in Africa and are the most varied of all seven serotypes, only two antigenic sites have been identified. We have followed a unique approach using an infectious SAT2 cDNA genome-length clone. Ten structurally surface-exposed, highly varied loops were identified as putative antigenic sites on the VP1, VP2, and VP3 capsid proteins of the SAT2/ZIM/7/83 virus. These regions were replaced with the corresponding regions of an antigenically disparate virus, SAT2/KNP/19/89. Antigenic profiling of the epitope-replaced and parental viruses with SAT2-specific MAbs led to the identification of two unique antibody-binding footprints on the SAT2 capsid. In this report, evidence for the structural engineering of antigenic sites of a SAT2 capsid to broaden cross-reactivity with antisera is provided." @default.
• W2117442345 created "2016-06-24" @default.
• W2117442345 creator A5002990635 @default.
• W2117442345 creator A5006736184 @default.
• W2117442345 creator A5016533281 @default.
• W2117442345 creator A5018189884 @default.
• W2117442345 creator A5047459331 @default.
• W2117442345 creator A5050798505 @default.
• W2117442345 creator A5063880751 @default.
• W2117442345 creator A5088198439 @default.
• W2117442345 date "2014-08-01" @default.
• W2117442345 modified "2023-09-25" @default.
• W2117442345 title "Determining the Epitope Dominance on the Capsid of a Serotype SAT2 Foot-and-Mouth Disease Virus by Mutational Analyses" @default.
• W2117442345 cites W1513186671 @default.
• W2117442345 cites W1673726422 @default.
• W2117442345 cites W1938043974 @default.
• W2117442345 cites W1969139732 @default.
• W2117442345 cites W1969474653 @default.
• W2117442345 cites W1969681931 @default.
• W2117442345 cites W1975040420 @default.
• W2117442345 cites W1976232165 @default.
• W2117442345 cites W1984026060 @default.
• W2117442345 cites W1984328279 @default.
• W2117442345 cites W1985909634 @default.
• W2117442345 cites W1987028100 @default.
• W2117442345 cites W1992410331 @default.
• W2117442345 cites W1992480829 @default.
• W2117442345 cites W1995213563 @default.
• W2117442345 cites W1999050844 @default.
• W2117442345 cites W2008547312 @default.
• W2117442345 cites W2024320790 @default.
• W2117442345 cites W2028390393 @default.
• W2117442345 cites W2034296843 @default.
• W2117442345 cites W2035838519 @default.
• W2117442345 cites W2037223418 @default.
• W2117442345 cites W2039475998 @default.
• W2117442345 cites W2041877620 @default.
• W2117442345 cites W2044162698 @default.
• W2117442345 cites W2044307883 @default.
• W2117442345 cites W2053492374 @default.
• W2117442345 cites W2057839080 @default.
• W2117442345 cites W2060913542 @default.
• W2117442345 cites W2065283382 @default.
• W2117442345 cites W2071390215 @default.
• W2117442345 cites W2075643158 @default.
• W2117442345 cites W2078118618 @default.
• W2117442345 cites W2079715545 @default.
• W2117442345 cites W2081380804 @default.
• W2117442345 cites W2095618519 @default.
• W2117442345 cites W2096803586 @default.
• W2117442345 cites W2097445077 @default.
• W2117442345 cites W2099413611 @default.
• W2117442345 cites W2100171893 @default.
• W2117442345 cites W2100701307 @default.
• W2117442345 cites W2100952660 @default.
• W2117442345 cites W2117593609 @default.
• W2117442345 cites W2118906352 @default.
• W2117442345 cites W2119862471 @default.
• W2117442345 cites W2139026373 @default.
• W2117442345 cites W2142656395 @default.
• W2117442345 cites W2153205607 @default.
• W2117442345 cites W2158459212 @default.
• W2117442345 cites W2160383645 @default.
• W2117442345 cites W2162776807 @default.
• W2117442345 cites W2165016728 @default.
• W2117442345 cites W2271580646 @default.
• W2117442345 cites W336318626 @default.
• W2117442345 doi "https://doi.org/10.1128/jvi.00470-14" @default.
• W2117442345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4135947" @default.
• W2117442345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24829347" @default.
• W2117442345 hasPublicationYear "2014" @default.
• W2117442345 type Work @default.
• W2117442345 sameAs 2117442345 @default.
• W2117442345 citedByCount "13" @default.
• W2117442345 countsByYear W21174423452015 @default.
• W2117442345 countsByYear W21174423452016 @default.
• W2117442345 countsByYear W21174423452017 @default.
• W2117442345 countsByYear W21174423452018 @default.
• W2117442345 countsByYear W21174423452020 @default.
• W2117442345 countsByYear W21174423452021 @default.
• W2117442345 countsByYear W21174423452022 @default.
• W2117442345 countsByYear W21174423452023 @default.
• W2117442345 crossrefType "journal-article" @default.
• W2117442345 hasAuthorship W2117442345A5002990635 @default.
• W2117442345 hasAuthorship W2117442345A5006736184 @default.
• W2117442345 hasAuthorship W2117442345A5016533281 @default.
• W2117442345 hasAuthorship W2117442345A5018189884 @default.
• W2117442345 hasAuthorship W2117442345A5047459331 @default.
• W2117442345 hasAuthorship W2117442345A5050798505 @default.
• W2117442345 hasAuthorship W2117442345A5063880751 @default.
• W2117442345 hasAuthorship W2117442345A5088198439 @default.
• W2117442345 hasBestOaLocation W21174423451 @default.
• W2117442345 hasConcept C14086860 @default.
• W2117442345 hasConcept C147483822 @default.
• W2117442345 hasConcept C159047783 @default.
• W2117442345 hasConcept C179223381 @default.
• W2117442345 hasConcept C195616568 @default.
• W2117442345 hasConcept C202878990 @default.

• next