Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117451125> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2117451125 endingPage "668" @default.
- W2117451125 startingPage "661" @default.
- W2117451125 abstract "alpha1,3galactosyltransferase (alpha1,3GalT) catalyzes the synthesis of a range of glycoconjugates containing the Galalpha1,3Gal epitope which is recognized by the naturally occurring human antibody, anti-Gal. This enzyme may be a useful synthetic tool to produce a range of compounds to further investigate the binding site of anti-Gal and other proteins with a Galalpha1,3Gal binding site. Thus, the enzyme has been probed with a series of type 2 disaccharide-C8(Galbeta1-4GlcNAc-C8) analogs. The enzyme tolerated acceptors with modifications at C2 and C3 of the N-acetylglucosamine residue, producing a family of compounds with a nonreducing alpha1,3 linked galactose. Compounds that did not serve as acceptors were evaluated as inhibitors. Interestingly, the type 1 disaccharide-C8, Galbeta1-3GlcNAc-C8, was a good inhibitor of the enzyme (Ki = 270 microM vs. Km = 190 microM for Galbeta1-4GlcNAc-C8). A potential photoprobe, based on a modified type 2 disaccharide (octyl 3-amino-3-deoxy-3-N-(2-diazo-3, 3, 3-trifluoropropionyl-beta-D-galactopyranosyl-(1, 4)-2-acetamindo-2-deoxy-beta-D-glycopyranoside, (DTFP-LacNAc-C8)), was evaluated as an inhibitor of alpha1,3GalT. alpha1,3GalT bound DTFP-LacNAc-C8 with an affinity (Ki = 300 microM) similar to that displayed by the enzyme for LacNAc-C8. Additional studies were done to determine the enzyme's ability to transfer a range of sugars from UDP-sugar donors. The results of these experiments demonstrated that alpha1,3GalT has a strict specificity for UDP-Gal. Finally, inactivation studies with various amino acid modifiers were done to obtain information on the importance of different types of amino acids for alpha1,3GalT activity." @default.
- W2117451125 created "2016-06-24" @default.
- W2117451125 creator A5034151691 @default.
- W2117451125 creator A5035820127 @default.
- W2117451125 creator A5044739995 @default.
- W2117451125 creator A5089653410 @default.
- W2117451125 creator A5089742992 @default.
- W2117451125 date "1999-07-01" @default.
- W2117451125 modified "2023-10-16" @default.
- W2117451125 title "Characterization of the substrate specificity of alpha1,3galactosyltransferase utilizing modified N-acetyllactosamine disaccharides" @default.
- W2117451125 cites W1514358033 @default.
- W2117451125 cites W1527876912 @default.
- W2117451125 cites W1528778858 @default.
- W2117451125 cites W1529507538 @default.
- W2117451125 cites W1540123184 @default.
- W2117451125 cites W1548459639 @default.
- W2117451125 cites W1553229561 @default.
- W2117451125 cites W1618744733 @default.
- W2117451125 cites W1812801726 @default.
- W2117451125 cites W1879336500 @default.
- W2117451125 cites W1978364683 @default.
- W2117451125 cites W2013645313 @default.
- W2117451125 cites W2036564231 @default.
- W2117451125 cites W2040666939 @default.
- W2117451125 cites W2042248138 @default.
- W2117451125 cites W2046860202 @default.
- W2117451125 cites W2048769067 @default.
- W2117451125 cites W2052330241 @default.
- W2117451125 cites W2059720167 @default.
- W2117451125 cites W2078854426 @default.
- W2117451125 cites W2089189488 @default.
- W2117451125 cites W2321295777 @default.
- W2117451125 cites W2476195779 @default.
- W2117451125 doi "https://doi.org/10.1093/glycob/9.7.661" @default.
- W2117451125 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10362835" @default.
- W2117451125 hasPublicationYear "1999" @default.
- W2117451125 type Work @default.
- W2117451125 sameAs 2117451125 @default.
- W2117451125 citedByCount "17" @default.
- W2117451125 countsByYear W21174511252012 @default.
- W2117451125 crossrefType "journal-article" @default.
- W2117451125 hasAuthorship W2117451125A5034151691 @default.
- W2117451125 hasAuthorship W2117451125A5035820127 @default.
- W2117451125 hasAuthorship W2117451125A5044739995 @default.
- W2117451125 hasAuthorship W2117451125A5089653410 @default.
- W2117451125 hasAuthorship W2117451125A5089742992 @default.
- W2117451125 hasConcept C13426554 @default.
- W2117451125 hasConcept C159654299 @default.
- W2117451125 hasConcept C181199279 @default.
- W2117451125 hasConcept C185592680 @default.
- W2117451125 hasConcept C195616568 @default.
- W2117451125 hasConcept C196032511 @default.
- W2117451125 hasConcept C203014093 @default.
- W2117451125 hasConcept C2777346074 @default.
- W2117451125 hasConcept C2778173381 @default.
- W2117451125 hasConcept C2781338088 @default.
- W2117451125 hasConcept C55493867 @default.
- W2117451125 hasConcept C71240020 @default.
- W2117451125 hasConcept C86803240 @default.
- W2117451125 hasConceptScore W2117451125C13426554 @default.
- W2117451125 hasConceptScore W2117451125C159654299 @default.
- W2117451125 hasConceptScore W2117451125C181199279 @default.
- W2117451125 hasConceptScore W2117451125C185592680 @default.
- W2117451125 hasConceptScore W2117451125C195616568 @default.
- W2117451125 hasConceptScore W2117451125C196032511 @default.
- W2117451125 hasConceptScore W2117451125C203014093 @default.
- W2117451125 hasConceptScore W2117451125C2777346074 @default.
- W2117451125 hasConceptScore W2117451125C2778173381 @default.
- W2117451125 hasConceptScore W2117451125C2781338088 @default.
- W2117451125 hasConceptScore W2117451125C55493867 @default.
- W2117451125 hasConceptScore W2117451125C71240020 @default.
- W2117451125 hasConceptScore W2117451125C86803240 @default.
- W2117451125 hasIssue "7" @default.
- W2117451125 hasLocation W21174511251 @default.
- W2117451125 hasLocation W21174511252 @default.
- W2117451125 hasOpenAccess W2117451125 @default.
- W2117451125 hasPrimaryLocation W21174511251 @default.
- W2117451125 hasRelatedWork W107855623 @default.
- W2117451125 hasRelatedWork W1966653929 @default.
- W2117451125 hasRelatedWork W1974085388 @default.
- W2117451125 hasRelatedWork W1985547429 @default.
- W2117451125 hasRelatedWork W2004712944 @default.
- W2117451125 hasRelatedWork W2060114429 @default.
- W2117451125 hasRelatedWork W2083140921 @default.
- W2117451125 hasRelatedWork W2949862966 @default.
- W2117451125 hasRelatedWork W3124980027 @default.
- W2117451125 hasRelatedWork W4205833803 @default.
- W2117451125 hasVolume "9" @default.
- W2117451125 isParatext "false" @default.
- W2117451125 isRetracted "false" @default.
- W2117451125 magId "2117451125" @default.
- W2117451125 workType "article" @default.