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- W2117514118 abstract "Background: Two-step derivatization procedures were developed for the enhancement of the positive ESI in LC–MS detection of anabolic androgenic steroids, a class of prohibited substances with limited ionization efficiency in atmospheric pressure interfaces. The developed procedures are based on the esterification of hydroxyl groups of anabolic steroids with picolinic acid, followed by conversion of carbonyl groups to Schiff bases by either Girard’s reagent T or 2-hydrazino pyridin. Results: Ionization efficiency for the model derivatized compounds 19-norandrosterone (nandrolone main metabolite) and methasterone was higher by almost two orders of magnitude compared with the respective efficiency of the underivatized compounds. Conclusion: The obtained derivatives provided a significant improvement in the ESI sensitivity, compared with those of underivatized molecules in positive LC–ESI-ion trap-MS full-scan mode." @default.
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- W2117514118 date "2012-01-01" @default.
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- W2117514118 title "Two-step derivatization procedures for the ionization enhancement of anabolic steroids in LC–ESI-MS for doping control analysis" @default.
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- W2117514118 doi "https://doi.org/10.4155/bio.11.308" @default.
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