FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117528325> ?p ?o ?g. }

• W2117528325 endingPage "1839" @default.
• W2117528325 startingPage "1829" @default.
• W2117528325 abstract "The aggregation of receptors with high affinity for IgE (FcepsilonRI) on the surface of mast cells and basophils initiates a chain of biochemical events culminating in the release of allergy mediators. Although microtubules have been implicated in the activation process, the molecular mechanism of their interactions with signal transduction molecules is poorly understood. Here we show that in rat basophilic leukemia cells large amounts of alphabeta-tubulin dimers ( approximately 70%) and gamma-tubulin ( approximately 85%) are found in a soluble pool which was released from the cells after permeabilization with saponin, or extraction with non-ionic detergents. Soluble tubulins were found in large complexes with other molecules. Complexes of soluble gamma-tubulin released from activated cells contained tyrosine-phosphorylated proteins of relative mol. wt approximately 25, 50, 53, 56, 60, 75, 80, 97, 115 and 200 kDa. Increased tyrosine phosphorylation of proteins associated with the cytoskeleton, i.e. around centrosomes, was detected by immunofluorescence microscopy. In vitro kinase assays revealed increased tyrosine phosphorylation of proteins in gamma-tubulin complexes isolated from activated cells. Two of the tyrosine phosphorylated proteins in these complexes were identified as the p53/56(lyn) kinase. Furthermore, gamma-tubulin bound to the N-terminal fragment of recombinant Lyn kinase and its binding was slightly enhanced in activated cells. Pretreatment of the cells with Src family-selective tyrosine kinase inhibitor, PP1, decreased the amount of tyrosine phosphorylated proteins in gamma-tubulin complexes, as well as the amount of gamma-tubulin in Lyn kinase immunocomplexes. The combined data suggest that gamma-tubulin is involved in early stages of mast cell activation." @default.
• W2117528325 created "2016-06-24" @default.
• W2117528325 creator A5011070297 @default.
• W2117528325 creator A5018075590 @default.
• W2117528325 creator A5018165739 @default.
• W2117528325 creator A5032505805 @default.
• W2117528325 creator A5074269101 @default.
• W2117528325 date "1999-11-01" @default.
• W2117528325 modified "2023-09-26" @default.
• W2117528325 title "Protein tyrosine kinase p53/p56lyn forms complexes with γ-tubulin in rat basophilic leukemia cells" @default.
• W2117528325 cites W1503251968 @default.
• W2117528325 cites W1573706815 @default.
• W2117528325 cites W1596581852 @default.
• W2117528325 cites W1605168381 @default.
• W2117528325 cites W1840837207 @default.
• W2117528325 cites W1904253008 @default.
• W2117528325 cites W1960680499 @default.
• W2117528325 cites W1964767880 @default.
• W2117528325 cites W1965703662 @default.
• W2117528325 cites W1982072152 @default.
• W2117528325 cites W1987359407 @default.
• W2117528325 cites W1990759267 @default.
• W2117528325 cites W2002603998 @default.
• W2117528325 cites W2009017737 @default.
• W2117528325 cites W2011243048 @default.
• W2117528325 cites W2012550113 @default.
• W2117528325 cites W2020952609 @default.
• W2117528325 cites W2023965621 @default.
• W2117528325 cites W2024203551 @default.
• W2117528325 cites W2024326013 @default.
• W2117528325 cites W2028847269 @default.
• W2117528325 cites W2031901918 @default.
• W2117528325 cites W2041772576 @default.
• W2117528325 cites W2042007591 @default.
• W2117528325 cites W2055140746 @default.
• W2117528325 cites W2060101293 @default.
• W2117528325 cites W2060922997 @default.
• W2117528325 cites W2069497859 @default.
• W2117528325 cites W2076659391 @default.
• W2117528325 cites W2082130010 @default.
• W2117528325 cites W2087992398 @default.
• W2117528325 cites W2092902000 @default.
• W2117528325 cites W2094263173 @default.
• W2117528325 cites W2099204510 @default.
• W2117528325 cites W2106217238 @default.
• W2117528325 cites W2125148239 @default.
• W2117528325 cites W2129558898 @default.
• W2117528325 cites W2130851152 @default.
• W2117528325 cites W2133388060 @default.
• W2117528325 cites W2152450698 @default.
• W2117528325 cites W2161275773 @default.
• W2117528325 cites W2164599175 @default.
• W2117528325 cites W2164682544 @default.
• W2117528325 cites W2431832318 @default.
• W2117528325 doi "https://doi.org/10.1093/intimm/11.11.1829" @default.
• W2117528325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10545487" @default.
• W2117528325 hasPublicationYear "1999" @default.
• W2117528325 type Work @default.
• W2117528325 sameAs 2117528325 @default.
• W2117528325 citedByCount "29" @default.
• W2117528325 countsByYear W21175283252012 @default.
• W2117528325 countsByYear W21175283252013 @default.
• W2117528325 countsByYear W21175283252014 @default.
• W2117528325 countsByYear W21175283252015 @default.
• W2117528325 countsByYear W21175283252017 @default.
• W2117528325 countsByYear W21175283252019 @default.
• W2117528325 crossrefType "journal-article" @default.
• W2117528325 hasAuthorship W2117528325A5011070297 @default.
• W2117528325 hasAuthorship W2117528325A5018075590 @default.
• W2117528325 hasAuthorship W2117528325A5018165739 @default.
• W2117528325 hasAuthorship W2117528325A5032505805 @default.
• W2117528325 hasAuthorship W2117528325A5074269101 @default.
• W2117528325 hasConcept C101544691 @default.
• W2117528325 hasConcept C108636557 @default.
• W2117528325 hasConcept C11960822 @default.
• W2117528325 hasConcept C153911025 @default.
• W2117528325 hasConcept C175818844 @default.
• W2117528325 hasConcept C185592680 @default.
• W2117528325 hasConcept C20418707 @default.
• W2117528325 hasConcept C2776165026 @default.
• W2117528325 hasConcept C2777553839 @default.
• W2117528325 hasConcept C42362537 @default.
• W2117528325 hasConcept C55493867 @default.
• W2117528325 hasConcept C62478195 @default.
• W2117528325 hasConcept C84425145 @default.
• W2117528325 hasConcept C86803240 @default.
• W2117528325 hasConcept C95444343 @default.
• W2117528325 hasConceptScore W2117528325C101544691 @default.
• W2117528325 hasConceptScore W2117528325C108636557 @default.
• W2117528325 hasConceptScore W2117528325C11960822 @default.
• W2117528325 hasConceptScore W2117528325C153911025 @default.
• W2117528325 hasConceptScore W2117528325C175818844 @default.
• W2117528325 hasConceptScore W2117528325C185592680 @default.
• W2117528325 hasConceptScore W2117528325C20418707 @default.
• W2117528325 hasConceptScore W2117528325C2776165026 @default.
• W2117528325 hasConceptScore W2117528325C2777553839 @default.
• W2117528325 hasConceptScore W2117528325C42362537 @default.
• W2117528325 hasConceptScore W2117528325C55493867 @default.

• next