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- W2117584108 abstract "1. Carboxymethyl-Chitin-Remazol Brilliant Violet (CM-Chitin-RBV) was used for a colorimetric assay of chitinase activity in Antarctic krill, Euphausia superba. Comparison with a reductimetric method by end-product detection was carried out by measuring FPLC elution profiles of krill crude extracts with both assays. Both profiles matched significantly. 2. Krill chitinase was highly specific to CM-Chitin-RBV. The assay was characterized by easy handling and a very high sensitivity compared to that of end-product detection. Hydrolysis of CM-Chitin-RBV by N-acetyl-β-d-glucosaminidase, β-glucosidase, β-galactosidase and N-acteyl-muraminidase was negligible. 3. The enzyme characteristics of chitinase from Antarctic krill using CM-Chitin-RBV were: pHopt = 7.5, Topt = 50–55°C, Ea = 52.1 kJ · mole−1, KM = 0.07 ± 0.01 mg · ml−1." @default.
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- W2117584108 title "A soluble, dye-labelled chitin derivative adapted for the assay of krill chitinase" @default.
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- W2117584108 doi "https://doi.org/10.1016/0305-0491(93)90104-d" @default.
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