FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117610041> ?p ?o ?g. }

• W2117610041 endingPage "235" @default.
• W2117610041 startingPage "227" @default.
• W2117610041 abstract "The purpose of the present study was to characterize the transport of dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulphate (DHEAS) into hepatocytes at physiological and pharmacological concentrations. Hepatocytes were isolated from female Sprague-Dawley rats by collagenase perfusion. Uptake of [3H]DHEA and [3H]DHEAS at increasing concentrations (3.5 nM-100 microM) was measured by the rapid filtration technique at 30 s intervals up to 120 s. The uptake of DHEAS by hepatocytes was saturable (Km = 17.0 microM; Vmax = 3.7 nmol/min/mg cell protein). In contrast, a specific saturable transport system for DHEA could not be detected in rat hepatocytes. It is suggested that DHEA enters the cell by diffusion. The uptake of DHEAS could be inhibited by antimycin A, carbonylcyanide-m-chlorophenylhydrazone, and dinitrophenol (inhibitors of the mitochondrial respiratory chain), by dinitrofluorobenzene and p-hydroxymercuribenzoate (NH2- and SH-blockers, respectively), and by monensin (Na(+)-specific ionophore). No inhibition was seen in the presence of ouabain (inhibitor of Na(+)-K(+)-ATPase) and phalloidin (inhibitor of cholate transport and actin-blocker). Interestingly, DHEAS uptake was inhibited by bile acids (cholate, taurocholate and glycocholate). Conversely, [3H]cholate uptake was strongly inhibited by DHEAS, which indicates a competition for the same carrier. Replacement of sodium ion with choline markedly decreased uptake velocity at pharmacological DHEAS concentrations. The results suggest that DHEAS uptake is a saturable, energy-dependent, carrier-mediated, partially Na(+)-dependent process, and that DHEAS may be taken up via the multispecific bile acid transport system." @default.
• W2117610041 created "2016-06-24" @default.
• W2117610041 creator A5000789410 @default.
• W2117610041 creator A5018359816 @default.
• W2117610041 date "1995-09-01" @default.
• W2117610041 modified "2023-09-26" @default.
• W2117610041 title "Transport of dehydroepiandrosterone and dehydroepiandrosterone sulphate into rat hepatocytes" @default.
• W2117610041 cites W137236905 @default.
• W2117610041 cites W1441355856 @default.
• W2117610041 cites W173619687 @default.
• W2117610041 cites W1964700113 @default.
• W2117610041 cites W1965405528 @default.
• W2117610041 cites W1965980581 @default.
• W2117610041 cites W1969460852 @default.
• W2117610041 cites W1982981101 @default.
• W2117610041 cites W1998011451 @default.
• W2117610041 cites W2009227871 @default.
• W2117610041 cites W2011411875 @default.
• W2117610041 cites W2015689418 @default.
• W2117610041 cites W2021433755 @default.
• W2117610041 cites W2023747738 @default.
• W2117610041 cites W2032442742 @default.
• W2117610041 cites W2055118634 @default.
• W2117610041 cites W2075917796 @default.
• W2117610041 cites W2114438201 @default.
• W2117610041 cites W2127721754 @default.
• W2117610041 cites W2143442076 @default.
• W2117610041 cites W2147347701 @default.
• W2117610041 cites W36676616 @default.
• W2117610041 cites W4232013776 @default.
• W2117610041 doi "https://doi.org/10.1016/0960-0760(95)00132-j" @default.
• W2117610041 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7577704" @default.
• W2117610041 hasPublicationYear "1995" @default.
• W2117610041 type Work @default.
• W2117610041 sameAs 2117610041 @default.
• W2117610041 citedByCount "23" @default.
• W2117610041 countsByYear W21176100412016 @default.
• W2117610041 countsByYear W21176100412023 @default.
• W2117610041 crossrefType "journal-article" @default.
• W2117610041 hasAuthorship W2117610041A5000789410 @default.
• W2117610041 hasAuthorship W2117610041A5018359816 @default.
• W2117610041 hasConcept C126322002 @default.
• W2117610041 hasConcept C134018914 @default.
• W2117610041 hasConcept C178790620 @default.
• W2117610041 hasConcept C185592680 @default.
• W2117610041 hasConcept C2776330855 @default.
• W2117610041 hasConcept C2776992908 @default.
• W2117610041 hasConcept C2777911890 @default.
• W2117610041 hasConcept C2778421351 @default.
• W2117610041 hasConcept C2778596009 @default.
• W2117610041 hasConcept C2778918659 @default.
• W2117610041 hasConcept C2779014961 @default.
• W2117610041 hasConcept C2779133857 @default.
• W2117610041 hasConcept C2780224938 @default.
• W2117610041 hasConcept C2780657110 @default.
• W2117610041 hasConcept C41625074 @default.
• W2117610041 hasConcept C537181965 @default.
• W2117610041 hasConcept C55493867 @default.
• W2117610041 hasConcept C71315377 @default.
• W2117610041 hasConcept C71924100 @default.
• W2117610041 hasConcept C86803240 @default.
• W2117610041 hasConceptScore W2117610041C126322002 @default.
• W2117610041 hasConceptScore W2117610041C134018914 @default.
• W2117610041 hasConceptScore W2117610041C178790620 @default.
• W2117610041 hasConceptScore W2117610041C185592680 @default.
• W2117610041 hasConceptScore W2117610041C2776330855 @default.
• W2117610041 hasConceptScore W2117610041C2776992908 @default.
• W2117610041 hasConceptScore W2117610041C2777911890 @default.
• W2117610041 hasConceptScore W2117610041C2778421351 @default.
• W2117610041 hasConceptScore W2117610041C2778596009 @default.
• W2117610041 hasConceptScore W2117610041C2778918659 @default.
• W2117610041 hasConceptScore W2117610041C2779014961 @default.
• W2117610041 hasConceptScore W2117610041C2779133857 @default.
• W2117610041 hasConceptScore W2117610041C2780224938 @default.
• W2117610041 hasConceptScore W2117610041C2780657110 @default.
• W2117610041 hasConceptScore W2117610041C41625074 @default.
• W2117610041 hasConceptScore W2117610041C537181965 @default.
• W2117610041 hasConceptScore W2117610041C55493867 @default.
• W2117610041 hasConceptScore W2117610041C71315377 @default.
• W2117610041 hasConceptScore W2117610041C71924100 @default.
• W2117610041 hasConceptScore W2117610041C86803240 @default.
• W2117610041 hasIssue "5-6" @default.
• W2117610041 hasLocation W21176100411 @default.
• W2117610041 hasLocation W21176100412 @default.
• W2117610041 hasOpenAccess W2117610041 @default.
• W2117610041 hasPrimaryLocation W21176100411 @default.
• W2117610041 hasRelatedWork W2013948416 @default.
• W2117610041 hasRelatedWork W2019690572 @default.
• W2117610041 hasRelatedWork W2056192651 @default.
• W2117610041 hasRelatedWork W2065631591 @default.
• W2117610041 hasRelatedWork W2067765841 @default.
• W2117610041 hasRelatedWork W2076624838 @default.
• W2117610041 hasRelatedWork W2086288557 @default.
• W2117610041 hasRelatedWork W2093416895 @default.
• W2117610041 hasRelatedWork W2117610041 @default.
• W2117610041 hasRelatedWork W4248197120 @default.
• W2117610041 hasVolume "54" @default.
• W2117610041 isParatext "false" @default.

• next