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- W2117632768 abstract "Abstract : Avian pathogenic Escherichia coli (APEC) are known to cause extraintestinal disease in poultry, leadingto substantial losses in the industry. IutA, iron-regulated aerobactin receptor is firmly associated with APEC. To assessthe potential of IutA to induce protective immune responses, attenuated Salmonella Typhimurium strain expressingIutA was constructed and administered orally to BALB/c mice. The IutA-specific immune responses were measuredwith sera, vaginal and fecal samples by an enzyme-linked immunosorbent assay. We found that the Salmonella-IutAvaccine induced significantly higher immune responses as compared to the control inoculated with the attenuated S.Typhimurium containing the plasmid only. The IutA-specific immune responses were increased by second immunizationat third week after initial immunization, whereas triple immunization induced lower immune responses than thoseinduced by the double immunization. The Salmonella-IutA vaccine induced a nature of immunity biased to the Th1-type, as judged by the ratio of IutA-specific IgG isotypes (IgG2a/IgG1). Overall, these results suggest that theSalmonella-IutA vaccine appear to be suitable candidate for a vaccine against APEC.Keywords : avian pathogenic Escherichia coli, boost immunization, IutA, oral vaccination" @default.
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- W2117632768 date "2013-09-30" @default.
- W2117632768 modified "2023-09-27" @default.
- W2117632768 title "Evaluation of systemic and mucosal immune responses in mice administered with recombinant Salmonella Typhimurium expressing IutA protein" @default.
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- W2117632768 doi "https://doi.org/10.14405/kjvr.2013.53.3.163" @default.
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