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• W2117645968 abstract "Herpes simplex virus 1 (HSV-1) ICP27 has been shown to interact with RNA polymerase II (RNAP II) holoenzyme. Here, we show that ICP27 interacts with the C-terminal domain (CTD) of RNAP II and that ICP27 mutants that cannot interact fail to relocalize RNAP II to viral transcription sites, suggesting a role for ICP27 in RNAP II recruitment. Using monoclonal antibodies specific for different phosphorylated forms of the RNAP II CTD, we found that the serine-2 phosphorylated form, which is found predominantly in elongating complexes, was not recruited to viral transcription sites. Further, there was an overall reduction in phosphoserine-2 staining. Western blot analysis revealed that there was a pronounced decrease in the phosphoserine-2 form and in overall RNAP II levels in lysates from cells infected with wild-type HSV-1. There was no appreciable difference in cdk9 levels, suggesting that protein degradation rather than dephosphorylation was occurring. Treatment of infected cells with proteasome inhibitors MG-132 and lactacystin prevented the decrease in the phosphoserine-2 form and in overall RNAP II levels; however, there was a concomitant decrease in the levels of several HSV-1 late proteins and in virus yield. Proteasomal degradation has been shown to resolve stalled RNAP II complexes at sites of DNA damage to allow 3' processing of transcripts. Thus, we propose that at later times of infection when robust transcription and DNA replication are occurring, elongating complexes may collide and proteasomal degradation may be required for resolution." @default.
• W2117645968 created "2016-06-24" @default.
• W2117645968 creator A5033617654 @default.
• W2117645968 creator A5072861069 @default.
• W2117645968 creator A5087442186 @default.
• W2117645968 creator A5087611149 @default.
• W2117645968 date "2006-04-01" @default.
• W2117645968 modified "2023-10-16" @default.
• W2117645968 title "ICP27 Interacts with the C-Terminal Domain of RNA Polymerase II and Facilitates Its Recruitment to Herpes Simplex Virus 1 Transcription Sites, Where It Undergoes Proteasomal Degradation during Infection" @default.
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• W2117645968 doi "https://doi.org/10.1128/jvi.80.7.3567-3581.2006" @default.
• W2117645968 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1440381" @default.
• W2117645968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16537625" @default.
• W2117645968 hasPublicationYear "2006" @default.
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