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- W2117695288 abstract "Abstract Based on a serum-free and CO2-independent culture of Toxoplasma gondii in vitro, a simple single arrangement mass production of trophozoites of the highly virulent BK strain of this parasite was developed. Starting simultaneously, 107 host cells (HEp-2) and 107 Toxoplasma trophozoites were cultured in one cell-factory® for 5 days, removing unattached cells, cell remnants and extracellular parasites by flushing daily. Each unit yielded about 0.5×109 parasites, which were almost free of any contamination with host cells and cell debris. As extensive manipulation of the cultures were avoided, a simple and reliable method for a mass production of Toxoplasma trophozoites has been developed, distinguishing itself by a low medium consumption and a low work-expenditure." @default.
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- W2117695288 date "1996-11-01" @default.
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- W2117695288 title "Toxoplasma gondii in vitro cultivation: economic and efficient mass production" @default.
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- W2117695288 doi "https://doi.org/10.1016/s0167-7012(96)00953-0" @default.
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