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• W2117787648 abstract "The investigation of genetic alterations in cancer-relatedgenes is useful for research, prognostic and therapeuticpurposes. However, the genetic heterogeneity that often occursduring tumour progression can make correct analysischallenging. The objective of this work has been to develop,evaluate and apply techniques that are sufficiently sensitiveand specific to detect and analyse genetic alterations in skintumours as well as in normal skin.Initially, a method based on laser-assisted microdissectionin combination with conventional dideoxy sequencing wasdeveloped and evaluated for the analysis of the p53 tumoursuppressor gene in small tissue samples. This method was shownto facilitate the analysis of single somatic cells fromhistologic tissue sections. In two subsequent studies themethod was used to analyse single cells to investigate theeffects of ultraviolet (UV) light on normal skin. Single p53immunoreactive and nonimmunoreactive cells from differentlayers of sunexposed skin, as well as skin protected fromexposure, were analysed for mutations in the p53 gene. Theresults revealed the structure of a clandestine p53 clone andprovided new insight into the possible events involved innormal differentiation by suggesting a role for allele dropout.The mutational effect of physiological doses of ultravioletlight A (UVA) on normal skin was then investigated by analysingthe p53 gene status in single immunoreactive cells at differenttime-points. Strong indications were found that UVA (even atlow doses) is indeed a mutagen and that its role should not bedisregarded in skin carcinogenesis.After slight modifications, the p53 mutation analysisstrategy was thenused to complement an x-chromosomeinactivation assay for investigation of basal cell cancer (BCC)clonality. The conclusion was that although the majority ofBCC’s are of monoclonal origin, an occasional tumour withapparently polyclonal origin exists. Finally, apyrosequencing-based mutation detection method was developedand evaluated for detection of hot-spot mutations in the N-rasgene of malignant melanoma. More than 80 melanoma metastasissamples were analysed by the standard approach of single strandconformation polymorphism analysis (SSCP)/DNA sequencing and bythis pyrosequencing strategy. Pyrosequencing was found to be agood alternative to SSCP/DNA sequencing and showed equivalentreproducibility and sensitivity in addition to being a simpleand rapid technique.Keywords:single cell, DNA sequencing, p53, mutation,UV, BCC, pyrosequencing, malignant melanoma, N-ras" @default.
• W2117787648 created "2016-06-24" @default.
• W2117787648 creator A5085999299 @default.
• W2117787648 date "2002-01-01" @default.
• W2117787648 modified "2023-09-27" @default.
• W2117787648 title "Detection and Analysis of Genetic Alterations in Normal Skin and Skin Tumours" @default.
• W2117787648 cites W112589308 @default.
• W2117787648 cites W113263723 @default.
• W2117787648 cites W113957129 @default.
• W2117787648 cites W114326730 @default.
• W2117787648 cites W12411709 @default.
• W2117787648 cites W129333160 @default.
• W2117787648 cites W138857909 @default.
• W2117787648 cites W1484882395 @default.
• W2117787648 cites W1494290222 @default.
• W2117787648 cites W1497506123 @default.
• W2117787648 cites W1504213308 @default.
• W2117787648 cites W1514180732 @default.
• W2117787648 cites W1520883967 @default.
• W2117787648 cites W1550806320 @default.
• W2117787648 cites W1558724780 @default.
• W2117787648 cites W1583721197 @default.
• W2117787648 cites W1589411629 @default.
• W2117787648 cites W159325311 @default.
• W2117787648 cites W1598998194 @default.
• W2117787648 cites W1624112230 @default.
• W2117787648 cites W1625139021 @default.
• W2117787648 cites W1629931862 @default.
• W2117787648 cites W1657827244 @default.
• W2117787648 cites W1658221551 @default.
• W2117787648 cites W169191484 @default.
• W2117787648 cites W170122044 @default.
• W2117787648 cites W1767203358 @default.
• W2117787648 cites W1811163336 @default.
• W2117787648 cites W1833056827 @default.
• W2117787648 cites W1847864430 @default.
• W2117787648 cites W1882441043 @default.
• W2117787648 cites W1896684000 @default.
• W2117787648 cites W1902758494 @default.
• W2117787648 cites W1903297883 @default.
• W2117787648 cites W1905215454 @default.
• W2117787648 cites W1910666512 @default.
• W2117787648 cites W1932933996 @default.
• W2117787648 cites W1961642912 @default.
• W2117787648 cites W1962875404 @default.
• W2117787648 cites W1964060678 @default.
• W2117787648 cites W1964558738 @default.
• W2117787648 cites W1965887677 @default.
• W2117787648 cites W1965958910 @default.
• W2117787648 cites W1966724428 @default.
• W2117787648 cites W1966789907 @default.
• W2117787648 cites W1966812451 @default.
• W2117787648 cites W1967041337 @default.
• W2117787648 cites W1967629365 @default.
• W2117787648 cites W1968322588 @default.
• W2117787648 cites W1969757078 @default.
• W2117787648 cites W1969953125 @default.
• W2117787648 cites W1971132591 @default.
• W2117787648 cites W1971199253 @default.
• W2117787648 cites W1972235402 @default.
• W2117787648 cites W1972264162 @default.
• W2117787648 cites W1972585533 @default.
• W2117787648 cites W1972775867 @default.
• W2117787648 cites W1973529204 @default.
• W2117787648 cites W1973997021 @default.
• W2117787648 cites W1974474498 @default.
• W2117787648 cites W1974830441 @default.
• W2117787648 cites W1975378179 @default.
• W2117787648 cites W1975731949 @default.
• W2117787648 cites W1977214627 @default.
• W2117787648 cites W1977223522 @default.
• W2117787648 cites W1978518304 @default.
• W2117787648 cites W1979883235 @default.
• W2117787648 cites W1980591242 @default.
• W2117787648 cites W1981665139 @default.
• W2117787648 cites W1983466619 @default.
• W2117787648 cites W1983623378 @default.
• W2117787648 cites W1984378131 @default.
• W2117787648 cites W1984445615 @default.
• W2117787648 cites W1984611504 @default.
• W2117787648 cites W1984671779 @default.
• W2117787648 cites W1984891867 @default.
• W2117787648 cites W1985266041 @default.
• W2117787648 cites W1985857590 @default.
• W2117787648 cites W1987061468 @default.
• W2117787648 cites W1987137459 @default.
• W2117787648 cites W1987139476 @default.
• W2117787648 cites W1987765580 @default.
• W2117787648 cites W1987802717 @default.
• W2117787648 cites W1988318122 @default.
• W2117787648 cites W1988514521 @default.
• W2117787648 cites W1988659744 @default.
• W2117787648 cites W1990896124 @default.
• W2117787648 cites W1991494214 @default.
• W2117787648 cites W1992248162 @default.
• W2117787648 cites W1992456495 @default.
• W2117787648 cites W1992595091 @default.
• W2117787648 cites W1992876574 @default.
• W2117787648 cites W1993358791 @default.
• W2117787648 cites W1994057432 @default.

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