FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117799299> ?p ?o ?g. }

• W2117799299 endingPage "3397" @default.
• W2117799299 startingPage "3387" @default.
• W2117799299 abstract "Site-directed photoaffinity cross-linking experiments were performed by using human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) mutants with unique cysteine residues at several positions (i.e., positions 65, 67, 70, and 74) in the fingers subdomain of the p66 subunit. Since neither the introduction of the unique cysteine residues into the fingers nor the modification of the SH groups of these residues with photoaffinity cross-linking reagents caused a significant decrease in the enzymatic activities of RT, we were able to use this system to measure distances between specific positions in the fingers domain of RT and double-stranded DNA. HIV-1 RT is quite flexible. There are conformational changes associated with binding of the normal substrates and nonnucleoside RT inhibitors (NNRTIs). Cross-linking was used to monitor intramolecular movements associated with binding of an NNRTI either in the presence or in the absence of an incoming deoxynucleoside triphosphate (dNTP). Binding an incoming dNTP at the polymerase active site decreased the efficiency of cross-linking but caused only modest changes in the preferred positions of cross-linking. This finding suggests that the fingers of p66 are closer to an extended template in the open configuration of the enzyme with the fingers away from the active site than in the closed configuration with the fingers in direct contact with the incoming dNTP. NNRTI binding caused increased cross-linking in experiments with diazirine reagents (especially with a diazirine reagent with a longer linker) and a moderate shift in the preferred sites of interaction with the template. Cross-linking occurred closer to the polymerase active site for RTs modified at positions 70 and 74. The effects of NNRTI binding were more pronounced in the absence of a bound dNTP; pretreatment of HIV-1 RT with an NNRTI reduced the effect of dNTP binding. These observations can be explained if the binding of NNRTI causes a decrease in the flexibility in the fingers subdomain of RT-NNRTI complex and a decrease in the distance from the fingers to the template extension." @default.
• W2117799299 created "2016-06-24" @default.
• W2117799299 creator A5041494942 @default.
• W2117799299 creator A5041556838 @default.
• W2117799299 creator A5063432648 @default.
• W2117799299 creator A5076685416 @default.
• W2117799299 creator A5088567277 @default.
• W2117799299 date "2004-04-01" @default.
• W2117799299 modified "2023-09-27" @default.
• W2117799299 title "Nonnucleoside Inhibitor Binding Affects the Interactions of the Fingers Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase with DNA" @default.
• W2117799299 cites W1576999979 @default.
• W2117799299 cites W1963535341 @default.
• W2117799299 cites W1970538088 @default.
• W2117799299 cites W1973711348 @default.
• W2117799299 cites W1980418635 @default.
• W2117799299 cites W1980734028 @default.
• W2117799299 cites W1984814223 @default.
• W2117799299 cites W1993514133 @default.
• W2117799299 cites W2008954911 @default.
• W2117799299 cites W2009581957 @default.
• W2117799299 cites W2013116612 @default.
• W2117799299 cites W2020708383 @default.
• W2117799299 cites W2023644431 @default.
• W2117799299 cites W2029695975 @default.
• W2117799299 cites W2054327421 @default.
• W2117799299 cites W2057448926 @default.
• W2117799299 cites W2058530732 @default.
• W2117799299 cites W2061563001 @default.
• W2117799299 cites W2062377493 @default.
• W2117799299 cites W2063381239 @default.
• W2117799299 cites W2065040389 @default.
• W2117799299 cites W2066074388 @default.
• W2117799299 cites W2068852852 @default.
• W2117799299 cites W2072282959 @default.
• W2117799299 cites W2077036660 @default.
• W2117799299 cites W2084702672 @default.
• W2117799299 cites W2091565871 @default.
• W2117799299 cites W2092786021 @default.
• W2117799299 cites W2092853022 @default.
• W2117799299 cites W2093174110 @default.
• W2117799299 cites W2093596482 @default.
• W2117799299 cites W2102098015 @default.
• W2117799299 cites W2103766655 @default.
• W2117799299 cites W2105267696 @default.
• W2117799299 cites W2126340181 @default.
• W2117799299 cites W2126644478 @default.
• W2117799299 cites W2136034321 @default.
• W2117799299 cites W2145785697 @default.
• W2117799299 cites W2148719011 @default.
• W2117799299 cites W2153193209 @default.
• W2117799299 cites W2157816705 @default.
• W2117799299 cites W2161043338 @default.
• W2117799299 cites W2166667597 @default.
• W2117799299 cites W2396989952 @default.
• W2117799299 cites W4231419782 @default.
• W2117799299 doi "https://doi.org/10.1128/jvi.78.7.3387-3397.2004" @default.
• W2117799299 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/371094" @default.
• W2117799299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15016861" @default.
• W2117799299 hasPublicationYear "2004" @default.
• W2117799299 type Work @default.
• W2117799299 sameAs 2117799299 @default.
• W2117799299 citedByCount "35" @default.
• W2117799299 countsByYear W21177992992012 @default.
• W2117799299 countsByYear W21177992992013 @default.
• W2117799299 countsByYear W21177992992014 @default.
• W2117799299 countsByYear W21177992992015 @default.
• W2117799299 countsByYear W21177992992016 @default.
• W2117799299 countsByYear W21177992992017 @default.
• W2117799299 countsByYear W21177992992019 @default.
• W2117799299 countsByYear W21177992992020 @default.
• W2117799299 countsByYear W21177992992021 @default.
• W2117799299 countsByYear W21177992992022 @default.
• W2117799299 crossrefType "journal-article" @default.
• W2117799299 hasAuthorship W2117799299A5041494942 @default.
• W2117799299 hasAuthorship W2117799299A5041556838 @default.
• W2117799299 hasAuthorship W2117799299A5063432648 @default.
• W2117799299 hasAuthorship W2117799299A5076685416 @default.
• W2117799299 hasAuthorship W2117799299A5088567277 @default.
• W2117799299 hasBestOaLocation W21177992991 @default.
• W2117799299 hasConcept C104292427 @default.
• W2117799299 hasConcept C104317684 @default.
• W2117799299 hasConcept C107824862 @default.
• W2117799299 hasConcept C111919701 @default.
• W2117799299 hasConcept C12554922 @default.
• W2117799299 hasConcept C153911025 @default.
• W2117799299 hasConcept C156719811 @default.
• W2117799299 hasConcept C181199279 @default.
• W2117799299 hasConcept C185592680 @default.
• W2117799299 hasConcept C2756471 @default.
• W2117799299 hasConcept C2778581200 @default.
• W2117799299 hasConcept C2779201268 @default.
• W2117799299 hasConcept C2780557392 @default.
• W2117799299 hasConcept C2780815831 @default.
• W2117799299 hasConcept C41008148 @default.
• W2117799299 hasConcept C41183919 @default.
• W2117799299 hasConcept C552990157 @default.
• W2117799299 hasConcept C55493867 @default.
• W2117799299 hasConcept C67705224 @default.

• next