FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2117944186> ?p ?o ?g. }

• W2117944186 endingPage "2591" @default.
• W2117944186 startingPage "2583" @default.
• W2117944186 abstract "Summary: Gibberella zeae is a pathogen of small grain crops and produces trichothecene mycotoxins in infected host tissue. The role of trichothecenes in the virulence of G. zeae was previously investigated using trichothecene-non-producing mutants that were generated via transformation-mediated disruption of a gene ( Tri5 ) that encodes the first enzyme in the trichothecene biosynthetic pathway. The mutants were less virulent on some hosts than the wild-type strain from which they were derived. Here, we used two approaches to determine whether the reduced virulence of mutants was due specifically to Tri5 disruption or to non-target effects caused by the transformation process. First, we generated a revertant from a Tri5 disruption mutant by allowing the mutant to pass through the sexual phase of its life cycle. In approximately 2% of the resulting progeny the disrupted Tri5 had reverted to wild-type; however, only one of three revertant progeny also regained the ability to produce trichothecenes. In the second approach, we complemented the Tri5 mutation in a disruption mutant by transforming the mutant with a plasmid carrying a functional copy of Tri5. In all transformants examined, the ability to produce trichothecenes was restored. The restoration of trichothecene production in the revertant progeny and in the complemented mutant was accompanied by restoration of wild-type or near wild-type levels of virulence on wheat seedlings (cultivar Wheaton). The results indicate that the reduced virulence of the mutants was caused by disruption of Tri5 rather than non-target effects resulting from the transformation process. The results also provide further evidence that trichothecenes contribute to the virulence of plant-pathogenic fungi." @default.
• W2117944186 created "2016-06-24" @default.
• W2117944186 creator A5012996967 @default.
• W2117944186 creator A5026610699 @default.
• W2117944186 creator A5040323737 @default.
• W2117944186 date "1997-08-01" @default.
• W2117944186 modified "2023-10-15" @default.
• W2117944186 title "Restoration of wild-type virulence to Tri5 disruption mutants of Gibberella zeae via gene reversion and mutant complementation" @default.
• W2117944186 cites W1006720600 @default.
• W2117944186 cites W1749230666 @default.
• W2117944186 cites W1847378301 @default.
• W2117944186 cites W1964158438 @default.
• W2117944186 cites W1970320291 @default.
• W2117944186 cites W1989243638 @default.
• W2117944186 cites W1991485523 @default.
• W2117944186 cites W2010944075 @default.
• W2117944186 cites W2021844063 @default.
• W2117944186 cites W2023317385 @default.
• W2117944186 cites W2024778420 @default.
• W2117944186 cites W2026273381 @default.
• W2117944186 cites W2031223490 @default.
• W2117944186 cites W2038139244 @default.
• W2117944186 cites W2056953003 @default.
• W2117944186 cites W2069144532 @default.
• W2117944186 cites W2076795549 @default.
• W2117944186 cites W2082581033 @default.
• W2117944186 cites W2133673943 @default.
• W2117944186 cites W2138357457 @default.
• W2117944186 cites W2166356970 @default.
• W2117944186 cites W2314194742 @default.
• W2117944186 cites W2316321187 @default.
• W2117944186 cites W2324015166 @default.
• W2117944186 cites W2326764183 @default.
• W2117944186 cites W2333761520 @default.
• W2117944186 cites W2563626818 @default.
• W2117944186 doi "https://doi.org/10.1099/00221287-143-8-2583" @default.
• W2117944186 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9274012" @default.
• W2117944186 hasPublicationYear "1997" @default.
• W2117944186 type Work @default.
• W2117944186 sameAs 2117944186 @default.
• W2117944186 citedByCount "92" @default.
• W2117944186 countsByYear W21179441862012 @default.
• W2117944186 countsByYear W21179441862013 @default.
• W2117944186 countsByYear W21179441862014 @default.
• W2117944186 countsByYear W21179441862015 @default.
• W2117944186 countsByYear W21179441862016 @default.
• W2117944186 countsByYear W21179441862017 @default.
• W2117944186 countsByYear W21179441862018 @default.
• W2117944186 countsByYear W21179441862019 @default.
• W2117944186 countsByYear W21179441862020 @default.
• W2117944186 countsByYear W21179441862021 @default.
• W2117944186 countsByYear W21179441862022 @default.
• W2117944186 countsByYear W21179441862023 @default.
• W2117944186 crossrefType "journal-article" @default.
• W2117944186 hasAuthorship W2117944186A5012996967 @default.
• W2117944186 hasAuthorship W2117944186A5026610699 @default.
• W2117944186 hasAuthorship W2117944186A5040323737 @default.
• W2117944186 hasBestOaLocation W21179441861 @default.
• W2117944186 hasConcept C104317684 @default.
• W2117944186 hasConcept C143065580 @default.
• W2117944186 hasConcept C188082640 @default.
• W2117944186 hasConcept C204241405 @default.
• W2117944186 hasConcept C207583985 @default.
• W2117944186 hasConcept C2776889654 @default.
• W2117944186 hasConcept C2777367657 @default.
• W2117944186 hasConcept C2778867309 @default.
• W2117944186 hasConcept C2781266966 @default.
• W2117944186 hasConcept C54355233 @default.
• W2117944186 hasConcept C60987743 @default.
• W2117944186 hasConcept C86803240 @default.
• W2117944186 hasConcept C89423630 @default.
• W2117944186 hasConceptScore W2117944186C104317684 @default.
• W2117944186 hasConceptScore W2117944186C143065580 @default.
• W2117944186 hasConceptScore W2117944186C188082640 @default.
• W2117944186 hasConceptScore W2117944186C204241405 @default.
• W2117944186 hasConceptScore W2117944186C207583985 @default.
• W2117944186 hasConceptScore W2117944186C2776889654 @default.
• W2117944186 hasConceptScore W2117944186C2777367657 @default.
• W2117944186 hasConceptScore W2117944186C2778867309 @default.
• W2117944186 hasConceptScore W2117944186C2781266966 @default.
• W2117944186 hasConceptScore W2117944186C54355233 @default.
• W2117944186 hasConceptScore W2117944186C60987743 @default.
• W2117944186 hasConceptScore W2117944186C86803240 @default.
• W2117944186 hasConceptScore W2117944186C89423630 @default.
• W2117944186 hasIssue "8" @default.
• W2117944186 hasLocation W21179441861 @default.
• W2117944186 hasOpenAccess W2117944186 @default.
• W2117944186 hasPrimaryLocation W21179441861 @default.
• W2117944186 hasRelatedWork W1606198076 @default.
• W2117944186 hasRelatedWork W2023086955 @default.
• W2117944186 hasRelatedWork W2102764932 @default.
• W2117944186 hasRelatedWork W2130126775 @default.
• W2117944186 hasRelatedWork W2162046720 @default.
• W2117944186 hasRelatedWork W2313770673 @default.
• W2117944186 hasRelatedWork W2328770644 @default.
• W2117944186 hasRelatedWork W2400099083 @default.
• W2117944186 hasRelatedWork W3172708970 @default.
• W2117944186 hasRelatedWork W4252057802 @default.

• next