FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118045310> ?p ?o ?g. }

• W2118045310 endingPage "6039" @default.
• W2118045310 startingPage "6027" @default.
• W2118045310 abstract "The E1 open reading frame (ORF) of bovine papillomavirus type 1 is required for the persistence of viral genomes as multicopy plasmid molecules in transformed rodent fibroblasts. E1 has been reported to contain two separate complementation groups (M and R, corresponding to N- and C-terminal domains, respectively) which regulate viral replication. However, E1 behaves as a single gene with respect to cell transformation and viral transcription. We examined the proteins translated from the entire ORF by using three antisera raised against E1 peptide or bacterial fusion proteins. The capacity of the whole ORF to encode a 72-kDa protein was demonstrated by translation of synthetic RNA in a reticulocyte lysate system, by microinjection of RNA into Xenopus oocytes, and by expression in recombinant baculoviruses and vaccinia viruses. In eucaryotic cells, this protein was found to be phosphorylated and targeted to the cell nucleus. In vitro translation also produced shorter peptides, containing only the E1 C-terminal domain, because of internal translation starts on the third and fourth methionine codons within E1 ORF. On the other hand, mammalian cells infected by vaccinia E1 recombinant virus contained additional larger E1 phosphoproteins (transient 85-kDa and stable 88-kDa species), likely representing processed forms of the 72-kDa species. The E1 72-kDa nuclear phosphoprotein was detected in bovine papillomavirus type 1-transformed cells. We report the biochemical characteristics of full-sized and truncated E1 proteins: (i) the C-terminal half of E1 ORF contains a phosphorylation site(s); (ii) the full-sized E1, but not the C-terminal protein, binds DNA, without indication for recognition of defined sequences, and critical determinants for this activity are likely confined to an N-terminal domain of the protein; (iii) covalent affinity labeling experiments performed on vaccinia virus-encoded E1 proteins with an ATP analog confirmed our previous observation of sequence similarities between the E1 C-terminal domain and the ATPase domain of simian virus 40 large T antigen." @default.
• W2118045310 created "2016-06-24" @default.
• W2118045310 creator A5013420744 @default.
• W2118045310 creator A5042334202 @default.
• W2118045310 creator A5055108087 @default.
• W2118045310 creator A5061757861 @default.
• W2118045310 date "1990-12-01" @default.
• W2118045310 modified "2023-10-18" @default.
• W2118045310 title "Proteins encoded by the bovine papillomavirus E1 open reading frame: expression in heterologous systems and in virally transformed cells" @default.
• W2118045310 cites W1484799046 @default.
• W2118045310 cites W1494195428 @default.
• W2118045310 cites W1495097197 @default.
• W2118045310 cites W1532305014 @default.
• W2118045310 cites W1545521112 @default.
• W2118045310 cites W1570361865 @default.
• W2118045310 cites W1571609716 @default.
• W2118045310 cites W1614632633 @default.
• W2118045310 cites W1628512331 @default.
• W2118045310 cites W1663499167 @default.
• W2118045310 cites W1740513083 @default.
• W2118045310 cites W1784713664 @default.
• W2118045310 cites W1807776979 @default.
• W2118045310 cites W1903403666 @default.
• W2118045310 cites W1943421922 @default.
• W2118045310 cites W1966124176 @default.
• W2118045310 cites W1969133697 @default.
• W2118045310 cites W1969365988 @default.
• W2118045310 cites W1973393468 @default.
• W2118045310 cites W1976491544 @default.
• W2118045310 cites W1977507783 @default.
• W2118045310 cites W1981872298 @default.
• W2118045310 cites W1992235627 @default.
• W2118045310 cites W1994647181 @default.
• W2118045310 cites W2009105413 @default.
• W2118045310 cites W2009785322 @default.
• W2118045310 cites W2010601153 @default.
• W2118045310 cites W2011632061 @default.
• W2118045310 cites W2018231215 @default.
• W2118045310 cites W2018289835 @default.
• W2118045310 cites W2019587052 @default.
• W2118045310 cites W2020515594 @default.
• W2118045310 cites W2022750428 @default.
• W2118045310 cites W2038775631 @default.
• W2118045310 cites W2038964449 @default.
• W2118045310 cites W2046328458 @default.
• W2118045310 cites W2049221234 @default.
• W2118045310 cites W2052186724 @default.
• W2118045310 cites W2057944550 @default.
• W2118045310 cites W2065557418 @default.
• W2118045310 cites W2076343527 @default.
• W2118045310 cites W2077903723 @default.
• W2118045310 cites W2085301445 @default.
• W2118045310 cites W2085633658 @default.
• W2118045310 cites W2085921481 @default.
• W2118045310 cites W2092876476 @default.
• W2118045310 cites W2092930172 @default.
• W2118045310 cites W2100837269 @default.
• W2118045310 cites W2104156428 @default.
• W2118045310 cites W2104584019 @default.
• W2118045310 cites W2117477363 @default.
• W2118045310 cites W2208707498 @default.
• W2118045310 cites W2403273495 @default.
• W2118045310 cites W71856133 @default.
• W2118045310 doi "https://doi.org/10.1128/jvi.64.12.6027-6039.1990" @default.
• W2118045310 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/248776" @default.
• W2118045310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2173778" @default.
• W2118045310 hasPublicationYear "1990" @default.
• W2118045310 type Work @default.
• W2118045310 sameAs 2118045310 @default.
• W2118045310 citedByCount "36" @default.
• W2118045310 countsByYear W21180453102013 @default.
• W2118045310 crossrefType "journal-article" @default.
• W2118045310 hasAuthorship W2118045310A5013420744 @default.
• W2118045310 hasAuthorship W2118045310A5042334202 @default.
• W2118045310 hasAuthorship W2118045310A5055108087 @default.
• W2118045310 hasAuthorship W2118045310A5061757861 @default.
• W2118045310 hasBestOaLocation W21180453101 @default.
• W2118045310 hasConcept C104317684 @default.
• W2118045310 hasConcept C123894998 @default.
• W2118045310 hasConcept C141231307 @default.
• W2118045310 hasConcept C153911025 @default.
• W2118045310 hasConcept C159047783 @default.
• W2118045310 hasConcept C167625842 @default.
• W2118045310 hasConcept C22744801 @default.
• W2118045310 hasConcept C2776781592 @default.
• W2118045310 hasConcept C2777061661 @default.
• W2118045310 hasConcept C40767141 @default.
• W2118045310 hasConcept C47289529 @default.
• W2118045310 hasConcept C54355233 @default.
• W2118045310 hasConcept C86803240 @default.
• W2118045310 hasConceptScore W2118045310C104317684 @default.
• W2118045310 hasConceptScore W2118045310C123894998 @default.
• W2118045310 hasConceptScore W2118045310C141231307 @default.
• W2118045310 hasConceptScore W2118045310C153911025 @default.
• W2118045310 hasConceptScore W2118045310C159047783 @default.
• W2118045310 hasConceptScore W2118045310C167625842 @default.
• W2118045310 hasConceptScore W2118045310C22744801 @default.
• W2118045310 hasConceptScore W2118045310C2776781592 @default.

• next