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- W2118049212 abstract "The effects of five Agrobacterium strains, two selection agents, and co-culture conditions were evaluated on the transformation efficiency of three melon lines (‘T111’, ‘PS8’, ‘PNY’) belonging to the cultivar Cucumis melo subsp. melo ‘Piel de Sapo’, the C. melo subsp. melo ‘Vedrantais’ line, and the accession C. melo subsp. agrestis ‘PI 161375’. Transformation efficiencies for accession ‘PI 161375’ (10.6 %) were higher than those obtained for ‘Piel de Sapo’ (0.1 %) or ‘Vedrantais’ (3.1 %). Among the ‘Piel de Sapo’ lines, the highest rates of transformation were observed with ‘T111’. Selection with pmi solved the problem of escapes encountered with nptII on ‘Piel de Sapo’. Results on the number of explants with GUS expression indicated that co-culture at 28oC gave a better response than at 23oC. The higher transformation efficiency of ‘PI 161375’ was not explained by its competence to Agrobacterium infection, determined by early GUS expression, but most probably by a higher regeneration capacity. With the objective of selecting a ‘Piel de Sapo’ line with a higher regeneration and transformation performance, a population of NILs, derived from a cross between the Korean accession ‘PI 161375’ and the ‘Piel de Sapo’ line ‘T111’, was evaluated for its regeneration capacity." @default.
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- W2118049212 date "2008-01-01" @default.
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- W2118049212 title "Improving the genetic transformation efficiency of Cucumis melo subsp. melo 'Piel de Sapo' via Agrobacterium 1" @default.
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