FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2118056251> ?p ?o ?g. }

• W2118056251 abstract "Abstract Background Microbial proteases are one of the most commercially valuable enzymes, of which the largest market share has been taken by subtilases or alkaline proteases of the Bacillus species. Despite a large amount of information on microbial proteases, a search for novel proteases with unique properties is still of interest for both basic and applied aspects of this highly complex class of enzymes. Oxidant stable proteases (OSPs) have been shown to have a wide application in the detergent and bleaching industries and recently have become one of the most attractive enzymes in various biotechnological applications. Results A gene encoding a novel member of the subtilase superfamily was isolated from Virgibacillus sp. SK37, a protease-producing bacterium isolated from Thai fish sauce fermentation. The gene was cloned by an activity-based screening of a genomic DNA expression library on Luria-Bertani (LB) agar plates containing 1 mM IPTG and 3% skim milk. Of the 100,000 clones screened, all six isolated positive clones comprised one overlapping open reading frame of 45% identity to the aprX gene from Bacillus species. This gene, designated aprX-sk37 was cloned into pET21d(+) and over-expressed in E. coli BL21(DE3). The enzyme product, designated AprX-SK37, was purified by an immobilized metal ion affinity chromatography to apparent homogeneity and characterized. The AprX-SK37 enzyme showed optimal catalytic conditions at pH 9.5 and 55°C, based on the azocasein assay containing 5 mM CaCl 2 . Maximum catalytic activity was found at 1 M NaCl with residual activity of 30% at 3 M NaCl. Thermal stability of the enzyme was also enhanced by 1 M NaCl. The enzyme was absolutely calcium-dependent, with optimal concentration of CaCl 2 at 15 mM. Inhibitory effects by phenylmethanesulfonyl fluoride and ethylenediaminetetraacetic acid indicated that this enzyme is a metal-dependent serine protease. The enzyme activity was sensitive towards reducing agents, urea, and SDS, but relatively stable up to 5% of H 2 O 2 . Phylogenetic analysis suggested that AprX-SK37 belongs to a novel family of the subtilase superfamily. We propose the name of this new family as alkaline serine protease-X (AprX). Conclusions The stability towards H 2 O 2 and moderately halo- and thermo-tolerant properties of the AprX-SK37 enzyme are attractive for various biotechnological applications." @default.
• W2118056251 created "2016-06-24" @default.
• W2118056251 creator A5012172105 @default.
• W2118056251 creator A5054210769 @default.
• W2118056251 creator A5089266909 @default.
• W2118056251 creator A5091122273 @default.
• W2118056251 date "2011-06-09" @default.
• W2118056251 modified "2023-10-09" @default.
• W2118056251 title "A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillussp. SK37" @default.
• W2118056251 cites W126109032 @default.
• W2118056251 cites W1515209736 @default.
• W2118056251 cites W1551083500 @default.
• W2118056251 cites W1619383772 @default.
• W2118056251 cites W1652464928 @default.
• W2118056251 cites W1859259876 @default.
• W2118056251 cites W1883093314 @default.
• W2118056251 cites W1971305937 @default.
• W2118056251 cites W1975224955 @default.
• W2118056251 cites W1979386768 @default.
• W2118056251 cites W1980285674 @default.
• W2118056251 cites W1987555193 @default.
• W2118056251 cites W1990564361 @default.
• W2118056251 cites W1991377039 @default.
• W2118056251 cites W1996936181 @default.
• W2118056251 cites W2003168448 @default.
• W2118056251 cites W2005108404 @default.
• W2118056251 cites W2006670577 @default.
• W2118056251 cites W2015204736 @default.
• W2118056251 cites W2031801854 @default.
• W2118056251 cites W2035413293 @default.
• W2118056251 cites W2040022355 @default.
• W2118056251 cites W2048846412 @default.
• W2118056251 cites W2060748546 @default.
• W2118056251 cites W2065384285 @default.
• W2118056251 cites W2069968843 @default.
• W2118056251 cites W2080013180 @default.
• W2118056251 cites W2086095324 @default.
• W2118056251 cites W2086194761 @default.
• W2118056251 cites W2089047063 @default.
• W2118056251 cites W2097184060 @default.
• W2118056251 cites W2097382368 @default.
• W2118056251 cites W2100882223 @default.
• W2118056251 cites W2101501929 @default.
• W2118056251 cites W2102628698 @default.
• W2118056251 cites W2110275611 @default.
• W2118056251 cites W2113490663 @default.
• W2118056251 cites W2125121305 @default.
• W2118056251 cites W2128152723 @default.
• W2118056251 cites W2130144859 @default.
• W2118056251 cites W2150143342 @default.
• W2118056251 cites W2156311145 @default.
• W2118056251 cites W2159261956 @default.
• W2118056251 cites W2165026145 @default.
• W2118056251 cites W4237491994 @default.
• W2118056251 cites W4243285095 @default.
• W2118056251 cites W4293247451 @default.
• W2118056251 cites W4320800236 @default.
• W2118056251 doi "https://doi.org/10.1186/1472-6750-11-65" @default.
• W2118056251 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3135529" @default.
• W2118056251 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21658261" @default.
• W2118056251 hasPublicationYear "2011" @default.
• W2118056251 type Work @default.
• W2118056251 sameAs 2118056251 @default.
• W2118056251 citedByCount "19" @default.
• W2118056251 countsByYear W21180562512013 @default.
• W2118056251 countsByYear W21180562512014 @default.
• W2118056251 countsByYear W21180562512016 @default.
• W2118056251 countsByYear W21180562512017 @default.
• W2118056251 countsByYear W21180562512018 @default.
• W2118056251 countsByYear W21180562512019 @default.
• W2118056251 countsByYear W21180562512020 @default.
• W2118056251 countsByYear W21180562512021 @default.
• W2118056251 countsByYear W21180562512022 @default.
• W2118056251 countsByYear W21180562512023 @default.
• W2118056251 crossrefType "journal-article" @default.
• W2118056251 hasAuthorship W2118056251A5012172105 @default.
• W2118056251 hasAuthorship W2118056251A5054210769 @default.
• W2118056251 hasAuthorship W2118056251A5089266909 @default.
• W2118056251 hasAuthorship W2118056251A5091122273 @default.
• W2118056251 hasBestOaLocation W21180562511 @default.
• W2118056251 hasConcept C104317684 @default.
• W2118056251 hasConcept C145770059 @default.
• W2118056251 hasConcept C167625842 @default.
• W2118056251 hasConcept C181199279 @default.
• W2118056251 hasConcept C182220744 @default.
• W2118056251 hasConcept C2776414213 @default.
• W2118056251 hasConcept C2776714187 @default.
• W2118056251 hasConcept C47289529 @default.
• W2118056251 hasConcept C55493867 @default.
• W2118056251 hasConcept C86803240 @default.
• W2118056251 hasConcept C89423630 @default.
• W2118056251 hasConceptScore W2118056251C104317684 @default.
• W2118056251 hasConceptScore W2118056251C145770059 @default.
• W2118056251 hasConceptScore W2118056251C167625842 @default.
• W2118056251 hasConceptScore W2118056251C181199279 @default.
• W2118056251 hasConceptScore W2118056251C182220744 @default.
• W2118056251 hasConceptScore W2118056251C2776414213 @default.
• W2118056251 hasConceptScore W2118056251C2776714187 @default.
• W2118056251 hasConceptScore W2118056251C47289529 @default.
• W2118056251 hasConceptScore W2118056251C55493867 @default.

• next